| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 26,666 | A→G | T130T (ACA→ACG) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 26,666 | 0 | A | G | 95.9% | 147.7 / ‑4.3 | 49 | T130T (ACA→ACG) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (26/21); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01 | |||||||||||
GTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCG > NC_000913/26431‑26901 | gTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtacagt > 1:225805/1‑251 (MQ=255) ccTTTATTGAGCAGATTCTCGAAGTACCGGGCTGCGCGATCATGATTTTCTCCGCACACCGTGGTTCTCAGGCCGTACGTACAGAAGCAAAAAGCCGTGATTGGCCGGTGT‑TCGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAATTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAATGTACGATGGGCCAGTACAGTAACCCGGaa < 1:255845/250‑1 (MQ=255) ttATAGAGCAGATTAGCGAAGTACCGGACTGCGCGATCCGGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTGTACGG‑GTGTTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCAGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAgg < 2:113837/250‑1 (MQ=255) tGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGa < 1:64902/250‑1 (MQ=255) gTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATc < 1:116129/251‑1 (MQ=255) cGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCTTATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCg > 1:9277/1‑251 (MQ=255) cgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCCGAACAGTACCCCGGAAAGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtg > 2:332989/1‑251 (MQ=255) gATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGACCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCCGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGCATCGCCGGACGAtgtgg > 2:198411/1‑250 (MQ=255) ccTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGa < 1:76728/251‑1 (MQ=255) tGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGaa > 1:36155/1‑250 (MQ=255) gATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAACGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAAc > 1:134307/1‑251 (MQ=255) ttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGa < 1:345866/250‑1 (MQ=255) ttCTCAGCACAAGGTGGTGCTCAGGCGGTACGATACGAGGCAAAAAGCTGCGATTTGACTGTGT‑TGGATGCCACCGAT‑GCGGTGGTGACCAAAGTGCATATGGAAGTCTCCCGCGTCAGCCGTCGGGGCGAAGAGGCGATTCTCATCGGCCACTCCGGGCCTCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTGGAATCGCCGGCCGATGTGTGGAAACTGa < 2:312589/250‑1 (MQ=255) ctcctcACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTTT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGCTGGGCAAGTACAGTAACCCGGAAGGGGGACTGGAGCTGGTCGAATCGCCggtcgtagagtggaacctgaatg > 2:314928/1‑230 (MQ=255) ctcCGCACTCGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCAGCGATTTGACGGTGT‑TTGATGCCACC‑GTGCCGCTGGTGACTAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTAGAATCg < 2:110077/226‑1 (MQ=255) ctcCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCg > 1:110077/1‑226 (MQ=255) cGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGACGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGACGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGACTGTATCTGGGCGAATCGCCGGACGATGTGTGGAAACTGTCGGTc > 2:80072/1‑250 (MQ=255) cacaCGTTGTTTCTCAGGCGGTACTTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaaa < 2:85968/251‑1 (MQ=255) tgtTTCTCAGGCGGTACGTAACGAAGCAAAAAACCGCGATTTGACGGAGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAAAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGCTGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGa > 1:221242/1‑249 (MQ=255) tttCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAag > 1:237944/1‑249 (MQ=255) tcAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGc > 1:110547/1‑251 (MQ=255) gTACGTAACGACGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCACCCGTCGTGGCGAAGAGCCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAGGGTACGAGGGGCCAGTACAGTAACCCGGAAGGGGGAATGTAACTGGTCGAATAGCCGGACGATGTGTGGAATCTGACGGACAACAAAGAAGGGaaacccccctt > 2:32282/1‑242 (MQ=255) aCGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCAGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttt < 1:117016/250‑1 (MQ=255) cgcaaCGAAGCAAAAAGCCGCTAGTTGACGGTTG‑TTGATGCCACCGGT‑CCTCTGGTGACCAAAGTGAATATGGAAGCCGCCCGCGCCAGCCGGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGTACGATGTGTGGAAACTGACGGTAAAAAACGAAGAGAATCTCTCCTTTAt < 2:249332/248‑1 (MQ=255) cGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTAt > 1:260422/1‑251 (MQ=255) aaCGAATCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATTGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTTGAATCTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGa < 1:101901/250‑1 (MQ=255) agccgcgATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCt < 1:216448/248‑1 (MQ=255) gccgcgATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCt < 1:382968/229‑1 (MQ=255) tGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGCGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGGGGCAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGGATCGCCGGCCGAAGCGTGGAAACTGACGGTCCAATACGGAGAGAAGCTATCCTTTATGCCCCAGCCCACGCTGTCGGTGGAt > 2:377044/1‑251 (MQ=255) gtgt‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGacac > 1:37504/1‑251 (MQ=255) aTGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAt < 1:100240/251‑1 (MQ=255) ccTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATc < 1:198411/251‑1 (MQ=255) ccTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGAATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGagc > 1:243327/1‑249 (MQ=255) cTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTAAGATGGGCCAGTATAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGAAACGTCTGATGTGATCg > 1:42445/1‑251 (MQ=255) gT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAc < 1:294981/251‑1 (MQ=255) ggccgttGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCg < 1:346440/243‑1 (MQ=255) cGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAcgcg > 1:381444/1‑251 (MQ=255) ggTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAA‑CGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGGGGATGACACGTCTGATGTGATCGACGCGCTGCg > 1:156064/1‑251 (MQ=255) aaaGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACg > 1:321567/1‑251 (MQ=255) ggAAGTCGCCCGCGCCAGACGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGCAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTAGCTGGTCGAATCGCCGGACGATGTGAGGAAAATGACGGACAAAAACGAAGAGAAGCTCTCCGTTAGAAACCAGACCACGCGGTCGGGGGATGAAACGTCTGATGTGATAGACGCGCTGCGTAAACGATTGCCGaaa > 2:350203/1‑250 (MQ=255) cGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTc < 1:8192/250‑1 (MQ=255) gccagccatcggggcgatgagtcgaTTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGGCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCCCGCTGTCGGTGGATGACACGTCTGATGGGATCGACGCGCGGCGGAAACTCTTCACGAAAATTGTCGGTCcgcg > 2:82458/25‑251 (MQ=255) cGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGa < 1:339434/251‑1 (MQ=255) tGGCGAAGAGTCGATTCGCATCGGCCCCGCCGGGCATCCGGAAGTGGAAGGGACGATGGGCCAGTTCAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTTACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGATGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATg < 2:64606/248‑1 (MQ=255) aagagtcgaTTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGc > 1:43558/9‑251 (MQ=255) gtcgaTTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACg < 1:339618/247‑1 (MQ=255) cGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTGCGCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTAcgc > 1:137580/1‑251 (MQ=255) ggAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGCTCGACGCGCGCCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAACGATGACATCTGCTACGCCACGACTAACCGTCAgtcatcggaaagcg > 2:152971/1‑238 (MQ=255) aGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtgtg > 1:243685/1‑154 (MQ=255) | GTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCG > NC_000913/26431‑26901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |