| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 26,606 | T→C | S110S (AGT→AGC) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 26,606 | 0 | T | C | 93.8% | 122.1 / ‑2.9 | 49 | S110S (AGT→AGC) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (25/20); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
ACCGATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTC > NC_000913/26369‑26843 | aCCGATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATATTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCACGATTTGACGGTGT‑TTGATGCCACCTGC‑CCGCTGGTGCCCAACGAGCATATGGAAGTCGCCCGCGCCATTCGCCGTGGCga > 2:103871/1‑249 (MQ=255) gATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCgcagcgt > 1:249332/1‑245 (MQ=255) gTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATtct > 1:143707/1‑251 (MQ=255) gTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCgg > 1:254319/1‑251 (MQ=255) aaCCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACTGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAATCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAGAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGAGTCTCATCGGCCACGCCGGGCatcc < 1:150993/251‑4 (MQ=255) cTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTCTCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAACGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGGTTCTCATCGGCCACGCCGGGCATCCggc > 2:363155/1‑248 (MQ=255) tgtgGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTgg > 1:303726/1‑251 (MQ=255) cGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAggta < 1:295588/251‑3 (MQ=255) gTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtacagt > 1:225805/1‑251 (MQ=255) ccTTTATTGAGCAGATTCTCGAAGTACCGGGCTGCGCGATCATGATTTTCTCCGCACACCGTGGTTCTCAGGCCGTACGTACAGAAGCAAAAAGCCGTGATTGGCCGGTGT‑TCGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAATTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAATGTACGATGGGCCAGTACAGTAACCCGGaa < 1:255845/250‑1 (MQ=255) ttATAGAGCAGATTAGCGAAGTACCGGACTGCGCGATCCGGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTGTACGG‑GTGTTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCAGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAgg < 2:113837/250‑1 (MQ=255) tGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGa < 1:64902/250‑1 (MQ=255) gTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATc < 1:116129/251‑1 (MQ=255) cGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCTTATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCg > 1:9277/1‑251 (MQ=255) cgATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCCGAACAGTACCCCGGAAAGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtg > 2:332989/1‑251 (MQ=255) gATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGACCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCCGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGCATCGCCGGACGAtgtgg > 2:198411/1‑250 (MQ=255) ccTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGa < 1:76728/251‑1 (MQ=255) tGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGaa > 1:36155/1‑250 (MQ=255) gATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAACGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAAc > 1:134307/1‑251 (MQ=255) ttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGa < 1:345866/250‑1 (MQ=255) ttCTCAGCACAAGGTGGTGCTCAGGCGGTACGATACGAGGCAAAAAGCTGCGATTTGACTGTGT‑TGGATGCCACCGAT‑GCGGTGGTGACCAAAGTGCATATGGAAGTCTCCCGCGTCAGCCGTCGGGGCGAAGAGGCGATTCTCATCGGCCACTCCGGGCCTCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTGGAATCGCCGGCCGATGTGTGGAAACTGa < 2:312589/250‑1 (MQ=255) ctcctcACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTTT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGCTGGGCAAGTACAGTAACCCGGAAGGGGGACTGGAGCTGGTCGAATCGCCggtcgtagagtggaacctgaatg > 2:314928/1‑230 (MQ=255) ctcCGCACTCGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCAGCGATTTGACGGTGT‑TTGATGCCACC‑GTGCCGCTGGTGACTAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAGCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTAGAATCg < 2:110077/226‑1 (MQ=255) ctcCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCg > 1:110077/1‑226 (MQ=255) cGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGACGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGACGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGACTGTATCTGGGCGAATCGCCGGACGATGTGTGGAAACTGTCGGTc > 2:80072/1‑250 (MQ=255) cacaCGTTGTTTCTCAGGCGGTACTTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaaa < 2:85968/251‑1 (MQ=255) tgtTTCTCAGGCGGTACGTAACGAAGCAAAAAACCGCGATTTGACGGAGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAAAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGCTGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGa > 1:221242/1‑249 (MQ=255) tttCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAag > 1:237944/1‑249 (MQ=255) tcAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGc > 1:110547/1‑251 (MQ=255) gTACGTAACGACGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCACCCGTCGTGGCGAAGAGCCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAGGGTACGAGGGGCCAGTACAGTAACCCGGAAGGGGGAATGTAACTGGTCGAATAGCCGGACGATGTGTGGAATCTGACGGACAACAAAGAAGGGaaacccccctt > 2:32282/1‑242 (MQ=255) aCGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCAGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttt < 1:117016/250‑1 (MQ=255) cgcaaCGAAGCAAAAAGCCGCTAGTTGACGGTTG‑TTGATGCCACCGGT‑CCTCTGGTGACCAAAGTGAATATGGAAGCCGCCCGCGCCAGCCGGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGTACGATGTGTGGAAACTGACGGTAAAAAACGAAGAGAATCTCTCCTTTAt < 2:249332/248‑1 (MQ=255) cGTAACGAAGCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTAt > 1:260422/1‑251 (MQ=255) aaCGAATCAAAAAGCCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATTGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTTGAATCTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGa < 1:101901/250‑1 (MQ=255) agccgcgATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCt < 1:216448/248‑1 (MQ=255) gccgcgATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCt < 1:382968/229‑1 (MQ=255) tGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGCGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGGGGCAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGGATCGCCGGCCGAAGCGTGGAAACTGACGGTCCAATACGGAGAGAAGCTATCCTTTATGCCCCAGCCCACGCTGTCGGTGGAt > 2:377044/1‑251 (MQ=255) gtgt‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGacac > 1:37504/1‑251 (MQ=255) aTGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAt < 1:100240/251‑1 (MQ=255) ccTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATc < 1:198411/251‑1 (MQ=255) ccTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGAATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGagc > 1:243327/1‑249 (MQ=255) cTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTAAGATGGGCCAGTATAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGAAACGTCTGATGTGATCg > 1:42445/1‑251 (MQ=255) gT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAc < 1:294981/251‑1 (MQ=255) ggccgttGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCg < 1:346440/243‑1 (MQ=255) cGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAcgcg > 1:381444/1‑251 (MQ=255) ggTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAA‑CGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGGGGATGACACGTCTGATGTGATCGACGCGCTGCg > 1:156064/1‑251 (MQ=255) aaaGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACg > 1:321567/1‑251 (MQ=255) ggAAGTCGCCCGCGCCAGACGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGCAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTAGCTGGTCGAATCGCCGGACGATGTGAGGAAAATGACGGACAAAAACGAAGAGAAGCTCTCCGTTAGAAACCAGACCACGCGGTCGGGGGATGAAACGTCTGATGTGATAGACGCGCTGCGTAAACGATTGCCGaaa > 2:350203/1‑250 (MQ=255) cGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTc < 1:8192/250‑1 (MQ=255) | ACCGATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGT‑TTGATGCCACCTGT‑CCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTC > NC_000913/26369‑26843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |