Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,677	G→A	L157L (TTG→TTA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,677	0	G	A	90.9%	55.4 / NA	24	L157L (TTG→TTA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (8/12);  minor base C (1/1);  total (11/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCG  >  NC_000913/25432‑25911                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gCGATGGGTATTGGCGGGATCCGGGCGGTGATGATGAATCGCTCGAAGGCCACGCAGAAGCAAAACAATATCGCTTACGCGCGGATTATGGGCGGCG‑CGCTGGGAAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTc                                                                                                                                                                                                                                       <  1:53460/251‑1 (MQ=255)     ttGGTATTAGCGTGATCATGGCAGAGCTGATGTATAGCTCGAAGGCAACGCAGACGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGATCGACCTCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCTGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGTAACTGCCACCTGTGTCGGGGCGACCTTGGTTGTGCTGGACGGTTTTTTCACTTCTat                                                                                                                                                                                                                                    >  2:166795/1‑249 (MQ=255)                gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTTCCTTCTTATGCGAAAAAAc                                                                                                                                                                                                                         >  2:115341/1‑250 (MQ=255)                  gATCCTGGCGTTGATGTTGTATCGCTTGAATGCCACGCAGACTCTAAACAATCTCGCTTCCGCGCGGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCCACCGCCTGTGGCTCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaat                                                                                                                                                                                                                      <  1:263219/251‑1 (MQ=255)                           ggTGATGATTTATCGCTCGAAGGCCACGCAGAAGTAAAACAATATCTCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAccc                                                                                                                                                                                                             <  1:206195/250‑1 (MQ=255)                                      aTCGCTCGAAGGCCCCGCAGAAGCAAAACAATTTCGTTTACGCTCTGATTATGGGCGGCG‑CGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcg                                                                                                                                                                                                  <  1:27810/251‑1 (MQ=255)                                                     cGCAGAAGCAAAAAAATACCGCTTACGCGCTGATTATTGGCGGCG‑CGCGGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                                                                                   <  1:372449/251‑1 (MQ=255)                                                         gAAGCTAAACAATTTCGCTTCCGCGTTGATTGTTGTCGGCGTCG‑TGGCCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATcc                                                                                                                                                                               <  1:59304/251‑1 (MQ=255)                                                              aaaaaaatctCGCTTACGCGCTGATTTTGGGCGGCG‑CGCTGG‑CAACCCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                                                                                          <  1:166795/242‑1 (MQ=255)                                                                       tCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                                                                                                 >  1:241452/1‑251 (MQ=255)                                                                                        attGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGGGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAACATGCCGGATGAGCTGCTGACGCAt                                                                                                                                                                                      >  2:88205/1‑213 (MQ=255)                                                                                         ttggggggcgggcggggCAACC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCAt                                                                                                                                                                                      <  1:88205/199‑1 (MQ=255)                                                                                           ggcggcG‑CGCTGGGCAAGC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCAACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAAAACTAATAAACCCTGCCGTATGCGAGGCTGACGCAGCTAATCCGGCCTACAGATTGCTGCGAAAACGTAGGCCggct                                                                                                                                             >  1:185312/1‑249 (MQ=255)                                                                                                       gggCAACC‑TGTTCGCCCGCCTGTTGCACGGCTTCGTTGTCGATATGAGCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAc                                                                                                                                  <  1:244832/251‑1 (MQ=255)                                                                                                       gggCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGATGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAc                                                                                                                                  >  1:333696/1‑251 (MQ=255)                                                                                                                 gTTCGTCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACTGCGGCGGCTGGCATTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTTTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCTGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                          <  1:186158/250‑1 (MQ=255)                                                                                                                               ggCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAACAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCAAAAATCCtt                                                                                                            >  1:202392/1‑250 (MQ=255)                                                                                                                                           ttGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaaaatc                                                                                                >  2:104345/1‑246 (MQ=255)                                                                                                                                                ggtatGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCt                                                                                          >  1:78758/3‑251 (MQ=255)                                                                                                                                                                     ggcggcTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACaga                                                                     <  1:298300/251‑1 (MQ=255)                                                                                                                                                                                     ccACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGCTAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCTTCCt                                                     >  1:200243/1‑251 (MQ=255)                                                                                                                                                                                                                      tgggTGAGGCACTGATTGTCTTGGAATGTTTTTTACCTTATAAAACGAAAAAACAATAACAAAACCTGCCGGATGCGATGCTGAAGCATCTTATCCGGCCTACAGATTGCTTCGAAATCGTAGGCCGGATAAGGTGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACATAGCAATAGCGCCGTCCGGGTGCACGTCACGCTAAAACTAGACGATGGca                     <  2:260947/248‑1 (MQ=255)                                                                                                                                                                                                                                      tgtgCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGACCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAACTGCATGTCTGAATCTGTACAGAGCAATATAGACGACCTGGTGCACTTCACGCTAACACTCGACGATGGCACAACCGCCGAGTCTAcc    >  2:81325/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCTGGAAGGTTTTTTCCCTTCTAAAGCGAAAAAACAATAATAACCCCTGCCGGATGGGATGCTGCCGAATTTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAACCCTTAAATATAAGAGCAACCCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCCCTCCACGCTAAACCCCGACGATGGCACCACCGCCGAGTCTACCCg  <  1:35389/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCG  >  NC_000913/25432‑25911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑