Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,598	A→G	D131G (GAC→GGC)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,598	0	A	G	100.0%	71.0 / NA	26	D131G (GAC→GGC)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

CGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGA  >  NC_000913/25371‑25844                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cgcgGCGTTTAGTTTCCTTGCCGATAGCGGAGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGGATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGGCCGCCTGTGGCACGGCTTCGTTGTCGATATGGTCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAAcc                                                                                                                                                                                                                                 >  1:100356/1‑251 (MQ=255)                     ccGATAGCGGCGGCTGGCAGTGTTGGTTTTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGTTGATTATTGGCGGCG‑CGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                                                                                                                                                                             <  1:275066/251‑1 (MQ=255)                             ggcggcGGGTAGGGTTGGTTCTTTGCGGGTATTGCTGTGGTTATTAGCGTGATCCTGGCAGTGATGTGGTATCGCTCGAAGGCCACGCAGAAGCTAAACATTATCGCTTACGTGTTGATTATTGGCGGCG‑CGTGGGGCAACC‑TTTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTTGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGc                                                                                                                                                                                                     <  1:39711/251‑1 (MQ=255)                                         cGTTGGATCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTCTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGCTATGATCGACTTCTACGTCGGCGGCTGGCCCTTCGCCACCTTCCACCTTGCCGATACTGCCCTCTGTGTCGGTTCCGCACTGATtgt                                                                                                                                                                                         >  2:35389/1‑251 (MQ=255)                                                              gCGATGGGTATTGGCGGGATCCGGGCGGTGATGATGAATCGCTCGAAGGCCACGCAGAAGCAAAACAATATCGCTTACGCGCGGATTATGGGCGGCG‑CGCTGGGAAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTc                                                                                                                                                                    <  1:53460/251‑1 (MQ=255)                                                                  ttGGTATTAGCGTGATCATGGCAGAGCTGATGTATAGCTCGAAGGCAACGCAGACGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGATCGACCTCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCTGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGTAACTGCCACCTGTGTCGGGGCGACCTTGGTTGTGCTGGACGGTTTTTTCACTTCTat                                                                                                                                                                 >  2:166795/1‑249 (MQ=255)                                                                      tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCAcc                                                                                                                                                                                                                                        >  1:362442/1‑175 (MQ=255)                                                                             gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTTCCTTCTTATGCGAAAAAAc                                                                                                                                                      >  2:115341/1‑250 (MQ=255)                                                                               gATCCTGGCGTTGATGTTGTATCGCTTGAATGCCACGCAGACTCTAAACAATCTCGCTTCCGCGCGGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCCACCGCCTGTGGCTCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTTTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaat                                                                                                                                                   <  1:263219/251‑1 (MQ=255)                                                                                        ggTGATGATTTATCGCTCGAAGGCCACGCAGAAGTAAAACAATATCTCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAccc                                                                                                                                          <  1:206195/250‑1 (MQ=255)                                                                                                   aTCGCTCGAAGGCCCCGCAGAAGCAAAACAATTTCGTTTACGCTCTGATTATGGGCGGCG‑CGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcg                                                                                                                               <  1:27810/251‑1 (MQ=255)                                                                                                                  cGCAGAAGCAAAAAAATACCGCTTACGCGCTGATTATTGGCGGCG‑CGCGGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCtt                                                                                                                <  1:372449/251‑1 (MQ=255)                                                                                                                      gAAGCTAAACAATTTCGCTTCCGCGTTGATTGTTGTCGGCGTCG‑TGGCCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATcc                                                                                                            <  1:59304/251‑1 (MQ=255)                                                                                                                           aaaaaaatctCGCTTACGCGCTGATTTTGGGCGGCG‑CGCTGG‑CAACCCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCt                                                                                                       <  1:166795/242‑1 (MQ=255)                                                                                                                                    tCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                              >  1:241452/1‑251 (MQ=255)                                                                                                                                       cTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                                                                                                                                                                                        >  2:181458/1‑126 (MQ=255)                                                                                                                                       cTTACGCGCTGATTATGGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                                                                                                                                                                                        <  1:181458/126‑1 (MQ=255)                                                                                                                                                     attGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGGGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAACATGCCGGATGAGCTGCTGACGCAt                                                                                                                   >  2:88205/1‑213 (MQ=255)                                                                                                                                                      ttggggggcgggcggggCAACC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCAt                                                                                                                   <  1:88205/199‑1 (MQ=255)                                                                                                                                                        ggcggcG‑CGCTGGGCAAGC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCAACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAAAACTAATAAACCCTGCCGTATGCGAGGCTGACGCAGCTAATCCGGCCTACAGATTGCTGCGAAAACGTAGGCCggct                                                                          >  1:185312/1‑249 (MQ=255)                                                                                                                                                                    gggCAACC‑TGTTCGCCCGCCTGTTGCACGGCTTCGTTGTCGATATGAGCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAc                                                               <  1:244832/251‑1 (MQ=255)                                                                                                                                                                    gggCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGATGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAc                                                               >  1:333696/1‑251 (MQ=255)                                                                                                                                                                              gTTCGTCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACTGCGGCGGCTGGCATTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTTTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCTGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                       <  1:186158/250‑1 (MQ=255)                                                                                                                                                                                            ggCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAACAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCAAAAATCCtt                                         >  1:202392/1‑250 (MQ=255)                                                                                                                                                                                                        ttGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaaaatc                             >  2:104345/1‑246 (MQ=255)                                                                                                                                                                                                             ggtatGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCt                       >  1:78758/3‑251 (MQ=255)                                                                                                                                                                                                                                  ggcggcTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACaga  <  1:298300/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CGCGGCGTTTAGTTTCCTTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCG‑CGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGA  >  NC_000913/25371‑25844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑