Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,211	A→T	A625A (GCA→GCT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,211	0	A	T	87.5%	82.2 / 1.3	32	A625A (GCA→GCT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base T (15/13);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTG  >  NC_000913/1974‑2424                                                                                                                                                                                                                                              |                                                                                                                                                                                                                      gctgAACCCGGTCATTGTTGACCGCACGTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAAtt                                                                                                                                                                                                            <  1:72705/249‑1 (MQ=255)   tgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACCAAAAGGCCAACACCTCGTCGATGGATTAATACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGAATtgatg                                                                                                                                                                                                        >  1:365732/1‑251 (MQ=255)       cccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCCCGTTGTCACGCCGACCAAAAAGGCCAACACCTCGTCGATGGATTACTCCCATCAGTTGTGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTCTGACACCAACGTTGGGGCTGGGTTACCGGTTATTGAGACCCTGCAAAATCTGCTCCATGTAGGTGATGCATTGATGAAGt                                                                                                                                                                                                    >  1:47531/1‑251 (MQ=255)          ggTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTGTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGTATTGATGAAGTtct                                                                                                                                                                                                 <  1:305939/251‑1 (MQ=255)           ttCATTGTTTACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATACCGACTTCTTGCGCGAAGGGTTCCACGTTGTCACGCCAAACAAAAAGGCCAAAACCTCGTCGTTGTATTAATACCATCTGTTAGGTCATACTGCGGAAAAATCGCGGAGTATATTACTATTAGAAAACAACGTTGGGACAGGATTAACGGTGATTGAGAAACCGAACAATCTGCACAGTGCTGGTAGTGAATTTATGAAgagctc                                                                                                                                                                                                >  2:264943/2‑246 (MQ=255)                         cACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttcag                                                                                                                                                                                  <  1:154121/251‑3 (MQ=255)                                   cagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTc                                                                                                                                                                        >  1:193580/1‑251 (MQ=255)                                                    atatGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGt                                                                                                                                                       >  1:62486/1‑251 (MQ=255)                                                     aatGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATTGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt                                                                                                                                                      <  2:62486/250‑1 (MQ=255)                                                      atGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt                                                                                                                                                      >  1:100147/1‑250 (MQ=255)                                                      atGCCGAATTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATATGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCACGttt                                                                                                                                                     >  2:161573/1‑250 (MQ=255)                                                         ccGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGAc                                                                                                                                                  <  1:37166/251‑1 (MQ=255)                                                                       gAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAACATCGCGGCGTCATTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGACACTGCAACATCTGCTCAATGCTGGTGATGAATTGATGACGTTCTCCGTCATTCTTTCGGGTCCGCTTTCTTATATCTTCGGTACGTTAGACGAAGGCATGAtttt                                                                                                                                    >  2:208696/1‑251 (MQ=255)                                                                       gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt                                                                                                                                    >  1:80426/1‑251 (MQ=255)                                                                        aaGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTtc                                                                                                                                   >  1:77929/1‑251 (MQ=255)                                                                                 actttGTCACGCCGACCAAAAAGGCCAACACCTCGTCGATGGATTACTACCATGAGTTGTGTTTTGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAGTGAGAACCTGCAAAATCGGCTCAATGCAGGTGATGAATTGATGAATTGCTCCGGCATTCGTTCTGGTGCGCTTTCGTATATCTTCGGGAAGTTAGGCGAAGGCATCAGTTGCTCCGAGGCg                                                                                                                          <  2:47531/248‑1 (MQ=255)                                                                                                aCAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATATTCGGCAAGTTAGACGAAGGCATGAGTTTATCCGAGGCGACTACTCTGGCGCgg                                                                                                           >  1:215758/1‑251 (MQ=255)                                                                                                              acCTCGTAGATGGATTACTACATTCTGTAGCGTCATGCGGAGGACAAATCGCGGCGTAAATTCCTCTATGACACCCACGTTGGGGCTGGATTACCGGTTACTGAGAACCTGCACCATTTGCTCAATGCTGGTGATGCATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGATAATGGGTTATAc                                                                                             >  2:142373/1‑251 (MQ=255)                                                                                                                 tcgtcgATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCg                                                                                           >  1:137882/1‑250 (MQ=255)                                                                                                                                   cATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatga                                                                        <  1:288394/251‑1 (MQ=255)                                                                                                                                           gCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTg                                                                <  1:237066/251‑1 (MQ=255)                                                                                                                                                    ggcggcAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTg                                                                >  1:102511/1‑242 (MQ=255)                                                                                                                                                    ggcggAAAAATCGCGGTGTAAATGCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTTATGAATTGATGAAGTTCTCCGGCAGTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTGAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGTGTTATACCGAACCGGATCCGCGAGATGATCTTTCTg                                                                <  2:102511/242‑1 (MQ=255)                                                                                                                                                           aaagcgcgGCGTAAATTCCGCTATGACTCCAACGTTGGGGCTGGATTACCGGTGAGTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCGTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGcgcg                                                <  2:100147/247‑1 (MQ=255)                                                                                                                                                             aTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTa                                              <  1:172498/251‑1 (MQ=255)                                                                                                                                                                               ctATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTctcgctc                            >  1:199118/1‑251 (MQ=255)                                                                                                                                                                                 aTGACACCAACGTTGGGTCTGGATTACCGGTTATTGAGATCCTGCAAAATCTGCTCAATGCTGGTTATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTTTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGATGCGAGTTCTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTctcgct                             <  1:343825/248‑1 (MQ=255)                                                                                                                                                                                            gTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGCTCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGGTTCTCGCTCGTGAAACGGGACg               >  2:114560/1‑251 (MQ=255)                                                                                                                                                                                            gTTGGAGCTGGCTTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGGTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATATTCGGCAAGTTAGACGAAGGCAGGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGACGCGGACCCGGGAGCTGATCTTTCTGGTATGGATGTGGCGCGTACACTTTTGGTTCTCCCTCGtcaacgaggagt               >  2:60592/1‑240 (MQ=255)                                                                                                                                                                                                  gCTGGATTAACGGGTATTGAGAACCTGCAAAATCTGCTCCATGCTGGTGATGAAATGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATAGCTTCGGTAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCTAGATGATCTGTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACt         <  2:50645/251‑1 (MQ=255)                                                                                                                                                                                                                               aaagcTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTg  <  2:73405/225‑1 (MQ=255)                                                                                                                                                                                                                               aaaTCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTg  >  1:73405/1‑229 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                      GCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTG  >  NC_000913/1974‑2424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑