Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,799	G→A	A803A (GCG→GCA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,799	0	G	A	94.6%	99.4 / ‑2.1	38	A803A (GCG→GCA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (12/23);  total (13/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTA  >  NC_000913/24555‑25045                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        agaTGGGTTCCTTCTACCTCGACATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTATCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAAc                                                                                                                                                                                                                                                   <  1:234293/251‑1 (MQ=255)            ttCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATAAACGATGCGTTCTg                                                                                                                                                                                                                                        >  1:29320/1‑251 (MQ=255)                    cGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAg                                                                                                                                                                                                                                 <  1:329928/250‑1 (MQ=255)                        atcagcAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGtt                                                                                                                                                                                                                            <  1:48118/251‑1 (MQ=255)                                   ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                                                                                                                                                                                 <  1:70206/251‑1 (MQ=255)                                         gtgcacCGCCAAGGGGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCTCAGAATCGCTGGTTCGCGGGATGGCACCAAACCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                                                                                                                                                                           <  2:288619/248‑1 (MQ=255)                                          tACACAGCCAAAGCGGACAGCGTGGAGCGGCGTAGCTGCCAGCCTGCGCTGTCTCACATCGCAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAGAAAACGTCATCACCGTGGAGTGGTACGCAGGCCGGTACGGTCAGGCAGCCAGTGAACCCAGGAACGATGCGTTATGGGACGGGCTGTTAAAAATGCGGGGCGAAgg                                                                                                                                                                                                          >  2:267587/1‑250 (MQ=255)                                                        ggACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                                                                                                                                                                            <  1:238202/251‑1 (MQ=255)                                                            agcgtgGCGCGTCGTAGCTGCCAGAATGCGCTGTATCACATCGCAGAAGCGCTGGCGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGGGGGGGGCTACCTGCCGGGCGAACGTGAAAATTACTTCTTCACCGGTGAGAGGTACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACTAAGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGTCGAAGAGAACAAAGACATAGAGc                                                                                                                                                                                         >  2:76316/4‑250 (MQ=255)                                                               gtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTCCGCTGGCTGGCACCAATCCTCTACTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAg‑c                                                                                                                                                                                    >  1:228072/1‑251 (MQ=255)                                                                     cgtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGc                                                                                                                                                                              >  1:252436/1‑251 (MQ=255)                                                                      gtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑AGCGTGcc                                                                                                                                                                             >  1:42673/1‑251 (MQ=255)                                                                         gtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGGGCTGGCTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGAc                                                                                                                                                                          >  1:310186/1‑251 (MQ=255)                                                                                ccAGACTGCGCTGTATCACATCGGAGAAGCGATGGTACGCAGGAAGGCACCAATCCTCTCCTTCACCGCTGATGAAGAGTGGGGCAAACCGCCAGGCGAAAGTGAAAAATACGTCTTCCCCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGGAGCGACATGAACAAAGGCATTGAG‑AAGACGCGTGGCGACAAGaa                                                                                                                                                                     >  2:226816/1‑249 (MQ=255)                                                                                  aGACTGAGCTGTATCACATTGTAGAAGCGCTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGTGCTACCTGCCGAGCGACCGTGACAAATTCGTCTTCACCGGTGAGTGATACGAAGGCCTTTGAGGTGTGGACGACAGTGACGCAATGTACGAGTCGTGCTGGGACGAGCTGATGCAAGTGCGTGGCGAAGCGAACAAAGTCATTGAGCAAG‑CGCGTGCCTATAAGaacgt                                                                                                                                                                  >  2:33408/1‑247 (MQ=255)                                                                                                cacgtcggataaGCGCTGGGGCGCTGGATGGCACCAAGCGGCTCCTGCACCGCTGATGAAGTGTGGGGCTACCGGCCGGGTGAACGTGAAAAATACGTCTGCACCGGTGAGTGGTACGCAGGCCTGTTTGGCCTGGCAGTCAGTGAAGCGAGGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCAATGAGCAAG‑CTCGTGCCGACAATAAAGTGGGTGGCGCGCTGGa                                                                                                                                                   <  2:153608/241‑1 (MQ=255)                                                                                                cacaTCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTgg                                                                                                                                                    <  1:171151/250‑1 (MQ=255)                                                                                                                                  cAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTg                                                                                                                 <  1:230040/251‑1 (MQ=255)                                                                                                                                               cACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGGTAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGGTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGa                                                                                                    <  2:312345/251‑1 (MQ=255)                                                                                                                                                      gatgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCt                                                                                             <  1:23790/251‑1 (MQ=255)                                                                                                                                                                ggggCTACCTGCCGGGCGAACGTGAAAAATACGTCTGCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGa                                                                                    <  1:232520/250‑1 (MQ=255)                                                                                                                                                                          gCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTg                                                                         <  1:353166/251‑1 (MQ=255)                                                                                                                                                                              ggCGAACGTGAAAAATACGTTTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGGAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCt                                                                     <  1:77037/251‑1 (MQ=255)                                                                                                                                                                                       gAAAAATACGTCTGCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCATG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCt                                                             <  1:88053/250‑1 (MQ=255)                                                                                                                                                                                              aCGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCt                                                     <  1:230468/251‑1 (MQ=255)                                                                                                                                                                                                 tCTTCACCGGTGAGTAGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGAGATGGGCGATGAATGACGATTTTGCTTGTTGACATCCTGCGCtccc                                                  >  2:287914/1‑248 (MQ=255)                                                                                                                                                                                                      cccGGTGAGTGGGACGAAGGGCTTGTTGGTCTGGCAATCAGTGAAGCAATGAACTAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGGATAAAGTCATTGTGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAGCCTTGTATGCAGAACCGGAACTGGCGGCGAACCTGAGCTCCCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGc                                             <  2:311396/250‑1 (MQ=255)                                                                                                                                                                                                         ggTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATACGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGc                                             >  1:287105/1‑248 (MQ=255)                                                                                                                                                                                                                ggTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAac                                   <  1:337561/251‑1 (MQ=255)                                                                                                                                                                                                                    cGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGaa                                                                                   <  1:205991/199‑1 (MQ=255)                                                                                                                                                                                                                                 ggtcTGGCAGACAGTGAAGCAATGAACGATACGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGc                  <  1:38522/248‑1 (MQ=255)                                                                                                                                                                                                                                  gtcTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCt                 <  1:206423/249‑1 (MQ=255)                                                                                                                                                                                                                                          gACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagcag           >  1:6955/1‑249 (MQ=255)                                                                                                                                                                                                                                           aCAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGCCTATAACGACGCACCTGCTGATGCTCAGCAGAGc        >  1:365993/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGaa     <  1:183847/251‑1 (MQ=255)                                                                                                                                                                                                                                                gAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGt   <  1:236918/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTa  <  1:163732/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTa  <  1:267587/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        AGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTA  >  NC_000913/24555‑25045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑