Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,670	G→T	V760V (GTG→GTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,670	0	G	T	88.9%	98.3 / 1.9	48	V760V (GTG→GTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (17/23);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGA  >  NC_000913/24425‑24900                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        gAAGAGAGGGTGGTACTGGACCGCTTGGCCGTAGGGTGTTCGAAAGCGGCACAGGAAGACATCATCAAGGCGTACGAAGCAGACGATTTCAACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTTCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCTTCGTAGCTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTg                                                                                                                                                                                                                                    <  2:241046/251‑1 (MQ=255)      gaTGGTGGTACTGGATCGCTGGGACGTAGGTTGTGCGAAAGAGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACATCGACATCATCACAGAACGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTACCAGACTGCGCTGTAACACATCGCAGAAGCGCTGGCTCGCTGGATgg                                                                                                                                                                                                                               >  1:242229/1‑251 (MQ=255)          gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCAcc                                                                                                                                                                                                                           >  1:282090/1‑251 (MQ=255)                  ggATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGTATGGCAACAATCctct                                                                                                                                                                                                                   >  2:77037/1‑251 (MQ=255)                       gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGAAGAAGCGCTGGTGCGCTGGATGGCACCAATCCACTCCTTc                                                                                                                                                                                                              >  2:152307/1‑251 (MQ=255)                       gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTc                                                                                                                                                                                                              <  1:96568/251‑1 (MQ=255)                       gCTGGGCCGTAGGGTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAAt                                                                                                                                                                                                                        <  1:351343/241‑1 (MQ=255)                           ggCCGTAGGTTATGCGAAAGCGGCACAGGAAGACATCCTCCAGGCGTACGAAGCATACGATTTCAACGAAGAGGTACAGCGACTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGAAGAAGCGCTGTCTCGCAGGATGGCACCActcctctcctacccct                                                                                                                                                                                                          >  2:307255/1‑245 (MQ=255)                                  ggTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCtgatga                                                                                                                                                                                                   <  1:173640/251‑1 (MQ=255)                                                 gCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCt                                                                                                                                                                                    <  1:70851/251‑1 (MQ=255)                                                  cacaGGAAGACAGCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTg                                                                                                                                                                                   <  1:372466/251‑1 (MQ=255)                                                    caGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGcc                                                                                                                                                                                 <  1:210366/251‑1 (MQ=255)                                                    caGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGcc                                                                                                                                                                                 <  1:289444/251‑1 (MQ=255)                                                        aaGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCAACCTGCCGGGc                                                                                                                                                                             <  1:152307/251‑1 (MQ=255)                                                         aGACATCCTCAAGGCGTAAGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCGCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCg                                                                                                                                                                            <  1:237570/251‑1 (MQ=255)                                                              tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGCTGAAGTGTGGGGCTACCTGCCGGGCGAACGt                                                                                                                                                                       >  1:170143/1‑251 (MQ=255)                                                               ccTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTg                                                                                                                                                                      <  1:37330/251‑1 (MQ=255)                                                                cTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCATCTGCTCCGTTGAGATGGGATCCTTCTACCTCGACATCATCAAAGTACGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCCGACTGCGCTGTATCACATCGCAGACGCGCTGGATCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTgg                                                                                                                                                                     >  2:329928/1‑250 (MQ=255)                                                                     ggCGTACGAAGCATCCGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCCCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAAt                                                                                                                                                                <  1:145134/251‑1 (MQ=255)                                                                        gTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCCCCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGCACGTGAAAAATAct                                                                                                                                                             >  2:308878/1‑250 (MQ=255)                                                                              aGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTc                                                                                                                                                        >  1:134808/1‑250 (MQ=255)                                                                                cattcGATTTCCACGAAGTGGTACAGCTTCTGACGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCGCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCAcc                                                                                                                                                     <  1:230444/247‑1 (MQ=255)                                                                                      aTTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAg                                                                                                                                               <  1:96142/251‑1 (MQ=255)                                                                                         tCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTg                                                                                                                                             <  1:100232/250‑1 (MQ=255)                                                                                                   ggtcaaGCGCCTGATGCGCTTCTTCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGcc                                                                                                                                  <  1:209253/246‑1 (MQ=255)                                                                                                       cAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATAAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCATAGCTGCCAGACTGCGCTGTATCACAGCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTACCCGCTGCTGAAATGTGGGGCTACCTGCCGGACGAACGTGAAAAATACGTCATCACCGGTGAGGGGTACGAAGGCCTGtt                                                                                                                              >  2:230468/1‑251 (MQ=255)                                                                                                               gATGCGCTTCTGCCCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACCTCGCAGAAGCGCTGGTTCGCTGGATGGAACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTgg                                                                                                                      >  2:353166/1‑251 (MQ=255)                                                                                                                       tCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGt                                                                                                              >  1:155458/1‑251 (MQ=255)                                                                                                                               gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGcaat                                                                                                      >  1:312345/1‑248 (MQ=255)                                                                                                                                   agaTGGGTTCCTTCTACCTCGACATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTATCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAAc                                                                                                  <  1:234293/251‑1 (MQ=255)                                                                                                                                              ttCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATAAACGATGCGTTCTg                                                                                       >  1:29320/1‑251 (MQ=255)                                                                                                                                                      cGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAg                                                                                <  1:329928/250‑1 (MQ=255)                                                                                                                                                          atcagcAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGtt                                                                           <  1:48118/251‑1 (MQ=255)                                                                                                                                                                     ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                                <  1:70206/251‑1 (MQ=255)                                                                                                                                                                           gtgcacCGCCAAGGGGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCTCAGAATCGCTGGTTCGCGGGATGGCACCAAACCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                          <  2:288619/248‑1 (MQ=255)                                                                                                                                                                            tACACAGCCAAAGCGGACAGCGTGGAGCGGCGTAGCTGCCAGCCTGCGCTGTCTCACATCGCAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAGAAAACGTCATCACCGTGGAGTGGTACGCAGGCCGGTACGGTCAGGCAGCCAGTGAACCCAGGAACGATGCGTTATGGGACGGGCTGTTAAAAATGCGGGGCGAAgg                                                         >  2:267587/1‑250 (MQ=255)                                                                                                                                                                                          ggACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                           <  1:238202/251‑1 (MQ=255)                                                                                                                                                                                              agcgtgGCGCGTCGTAGCTGCCAGAATGCGCTGTATCACATCGCAGAAGCGCTGGCGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGGGGGGGGCTACCTGCCGGGCGAACGTGAAAATTACTTCTTCACCGGTGAGAGGTACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACTAAGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGTCGAAGAGAACAAAGACATAGAGc                                        >  2:76316/4‑250 (MQ=255)                                                                                                                                                                                                 gtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTCCGCTGGCTGGCACCAATCCTCTACTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAg‑c                                   >  1:228072/1‑251 (MQ=255)                                                                                                                                                                                                       cgtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGc                             >  1:252436/1‑251 (MQ=255)                                                                                                                                                                                                        gtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑AGCGTGcc                            >  1:42673/1‑251 (MQ=255)                                                                                                                                                                                                           gtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGGGCTGGCTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGAc                         >  1:310186/1‑251 (MQ=255)                                                                                                                                                                                                                  ccAGACTGCGCTGTATCACATCGGAGAAGCGATGGTACGCAGGAAGGCACCAATCCTCTCCTTCACCGCTGATGAAGAGTGGGGCAAACCGCCAGGCGAAAGTGAAAAATACGTCTTCCCCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGGAGCGACATGAACAAAGGCATTGAG‑AAGACGCGTGGCGACAAGaa                    >  2:226816/1‑249 (MQ=255)                                                                                                                                                                                                                    aGACTGAGCTGTATCACATTGTAGAAGCGCTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGTGCTACCTGCCGAGCGACCGTGACAAATTCGTCTTCACCGGTGAGTGATACGAAGGCCTTTGAGGTGTGGACGACAGTGACGCAATGTACGAGTCGTGCTGGGACGAGCTGATGCAAGTGCGTGGCGAAGCGAACAAAGTCATTGAGCAAG‑CGCGTGCCTATAAGaacgt                 >  2:33408/1‑247 (MQ=255)                                                                                                                                                                                                                                  cacgtcggataaGCGCTGGGGCGCTGGATGGCACCAAGCGGCTCCTGCACCGCTGATGAAGTGTGGGGCTACCGGCCGGGTGAACGTGAAAAATACGTCTGCACCGGTGAGTGGTACGCAGGCCTGTTTGGCCTGGCAGTCAGTGAAGCGAGGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCAATGAGCAAG‑CTCGTGCCGACAATAAAGTGGGTGGCGCGCTGGa  <  2:153608/241‑1 (MQ=255)                                                                                                                                                                                                                                  cacaTCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTgg   <  1:171151/250‑1 (MQ=255)                                                                                                                                                                                                                                   acaccGCAGAAGCGCCGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAg                                                                                                          <  2:282876/143‑1 (MQ=255)                                                                                                                                                                                                                                   acaTCGCAGAAGCGATGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAg                                                                                                          >  1:282876/1‑147 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        GAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGA  >  NC_000913/24425‑24900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑