Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,646	A→G	L752L (CTA→CTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,646	0	A	G	94.2%	144.7 / ‑0.8	52	L752L (CTA→CTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (23/26);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGT  >  NC_000913/24402‑24885                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATcac                                                                                                                                                                                                                                            <  1:310667/251‑1 (MQ=255)       aaGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGc                                                                                                                                                                                                                                       >  1:288619/1‑250 (MQ=255)        aGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGTCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAg                                                                                                                                                                                                                                     <  1:354819/251‑1 (MQ=255)         gATATGGAGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGACGTAGGTTGTGCGAAAGCGGCACCGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTCCAGCGTCTGATGCGCGTCTGCTCCGTTGAGCTGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCACAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGCCTGCGCTGTATCACATCGc                                                                                                                                                                                                                                       >  2:23103/1‑248 (MQ=255)          atatGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCACTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGCATCACATCGCAGa                                                                                                                                                                                                                                    >  1:26475/1‑250 (MQ=255)            atGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAg                                                                                                                                                                                                                                  <  1:311613/250‑1 (MQ=255)               gTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCt                                                                                                                                                                                                                              <  1:235758/251‑1 (MQ=255)                     ccGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGttcg                                                                                                                                                                                                                        <  1:335211/251‑4 (MQ=255)                        gAAGAGAGGGTGGTACTGGACCGCTTGGCCGTAGGGTGTTCGAAAGCGGCACAGGAAGACATCATCAAGGCGTACGAAGCAGACGATTTCAACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTTCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCTTCGTAGCTGCCAGACTGCGCTGTATCACATCGCATAAGCGCTGGTTCGCTg                                                                                                                                                                                                                     <  2:241046/251‑1 (MQ=255)                             gaTGGTGGTACTGGATCGCTGGGACGTAGGTTGTGCGAAAGAGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACATCGACATCATCACAGAACGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTACCAGACTGCGCTGTAACACATCGCAGAAGCGCTGGCTCGCTGGATgg                                                                                                                                                                                                                >  1:242229/1‑251 (MQ=255)                                 gtggtACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCAcc                                                                                                                                                                                                            >  1:282090/1‑251 (MQ=255)                                         ggATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGTATGGCAACAATCctct                                                                                                                                                                                                    >  2:77037/1‑251 (MQ=255)                                              gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGAAGAAGCGCTGGTGCGCTGGATGGCACCAATCCACTCCTTc                                                                                                                                                                                               >  2:152307/1‑251 (MQ=255)                                              gCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTc                                                                                                                                                                                               <  1:96568/251‑1 (MQ=255)                                              gCTGGGCCGTAGGGTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAAt                                                                                                                                                                                                         <  1:351343/241‑1 (MQ=255)                                                  ggCCGTAGGTTATGCGAAAGCGGCACAGGAAGACATCCTCCAGGCGTACGAAGCATACGATTTCAACGAAGAGGTACAGCGACTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAACGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGAAGAAGCGCTGTCTCGCAGGATGGCACCActcctctcctacccct                                                                                                                                                                                           >  2:307255/1‑245 (MQ=255)                                                         ggTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCtgatga                                                                                                                                                                                    <  1:173640/251‑1 (MQ=255)                                                                        gCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCt                                                                                                                                                                     <  1:70851/251‑1 (MQ=255)                                                                         cacaGGAAGACAGCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTg                                                                                                                                                                    <  1:372466/251‑1 (MQ=255)                                                                           caGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGcc                                                                                                                                                                  <  1:210366/251‑1 (MQ=255)                                                                           caGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGcc                                                                                                                                                                  <  1:289444/251‑1 (MQ=255)                                                                               aaGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCAACCTGCCGGGc                                                                                                                                                              <  1:152307/251‑1 (MQ=255)                                                                                aGACATCCTCAAGGCGTAAGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCGCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCg                                                                                                                                                             <  1:237570/251‑1 (MQ=255)                                                                                     tCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGCTGAAGTGTGGGGCTACCTGCCGGGCGAACGt                                                                                                                                                        >  1:170143/1‑251 (MQ=255)                                                                                      ccTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTg                                                                                                                                                       <  1:37330/251‑1 (MQ=255)                                                                                       cTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCATCTGCTCCGTTGAGATGGGATCCTTCTACCTCGACATCATCAAAGTACGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCCGACTGCGCTGTATCACATCGCAGACGCGCTGGATCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTgg                                                                                                                                                      >  2:329928/1‑250 (MQ=255)                                                                                            ggCGTACGAAGCATCCGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCCCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAAt                                                                                                                                                 <  1:145134/251‑1 (MQ=255)                                                                                               gTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCCCCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGCACGTGAAAAATAct                                                                                                                                              >  2:308878/1‑250 (MQ=255)                                                                                                     aGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTc                                                                                                                                         >  1:134808/1‑250 (MQ=255)                                                                                                       cattcGATTTCCACGAAGTGGTACAGCTTCTGACGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCGCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCAcc                                                                                                                                      <  1:230444/247‑1 (MQ=255)                                                                                                             aTTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAg                                                                                                                                <  1:96142/251‑1 (MQ=255)                                                                                                                tCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTg                                                                                                                              <  1:100232/250‑1 (MQ=255)                                                                                                                          ggtcaaGCGCCTGATGCGCTTCTTCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGcc                                                                                                                   <  1:209253/246‑1 (MQ=255)                                                                                                                              cAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATAAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCATAGCTGCCAGACTGCGCTGTATCACAGCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTACCCGCTGCTGAAATGTGGGGCTACCTGCCGGACGAACGTGAAAAATACGTCATCACCGGTGAGGGGTACGAAGGCCTGtt                                                                                                               >  2:230468/1‑251 (MQ=255)                                                                                                                                      gATGCGCTTCTGCCCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACCTCGCAGAAGCGCTGGTTCGCTGGATGGAACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTgg                                                                                                       >  2:353166/1‑251 (MQ=255)                                                                                                                                              tCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGt                                                                                               >  1:155458/1‑251 (MQ=255)                                                                                                                                                      gTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGcaat                                                                                       >  1:312345/1‑248 (MQ=255)                                                                                                                                                          agaTGGGTTCCTTCTACCTCGACATCAGCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTATCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAAc                                                                                   <  1:234293/251‑1 (MQ=255)                                                                                                                                                                     ttCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATAAACGATGCGTTCTg                                                                        >  1:29320/1‑251 (MQ=255)                                                                                                                                                                             cGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAg                                                                 <  1:329928/250‑1 (MQ=255)                                                                                                                                                                                 atcagcAAAGCCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGtt                                                            <  1:48118/251‑1 (MQ=255)                                                                                                                                                                                            ccGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTg                                                 <  1:70206/251‑1 (MQ=255)                                                                                                                                                                                                  gtgcacCGCCAAGGGGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCTCAGAATCGCTGGTTCGCGGGATGGCACCAAACCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                           <  2:288619/248‑1 (MQ=255)                                                                                                                                                                                                   tACACAGCCAAAGCGGACAGCGTGGAGCGGCGTAGCTGCCAGCCTGCGCTGTCTCACATCGCAGAAGCGCTGGTACGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAGAAAACGTCATCACCGTGGAGTGGTACGCAGGCCGGTACGGTCAGGCAGCCAGTGAACCCAGGAACGATGCGTTATGGGACGGGCTGTTAAAAATGCGGGGCGAAgg                                          >  2:267587/1‑250 (MQ=255)                                                                                                                                                                                                                 ggACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                            <  1:238202/251‑1 (MQ=255)                                                                                                                                                                                                                     agcgtgGCGCGTCGTAGCTGCCAGAATGCGCTGTATCACATCGCAGAAGCGCTGGCGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGGGGGGGGCTACCTGCCGGGCGAACGTGAAAATTACTTCTTCACCGGTGAGAGGTACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACTAAGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGTCGAAGAGAACAAAGACATAGAGc                         >  2:76316/4‑250 (MQ=255)                                                                                                                                                                                                                        gtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTCCGCTGGCTGGCACCAATCCTCTACTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGCGCAAg‑c                    >  1:228072/1‑251 (MQ=255)                                                                                                                                                                                                                              cgtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGc              >  1:252436/1‑251 (MQ=255)                                                                                                                                                                                                                               gtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑AGCGTGcc             >  1:42673/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGGGCTGGCTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGAc          >  1:310186/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccAGACTGCGCTGTATCACATCGGAGAAGCGATGGTACGCAGGAAGGCACCAATCCTCTCCTTCACCGCTGATGAAGAGTGGGGCAAACCGCCAGGCGAAAGTGAAAAATACGTCTTCCCCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGTGCTGTTGAAAGTGCGGAGCGACATGAACAAAGGCATTGAG‑AAGACGCGTGGCGACAAGaa     >  2:226816/1‑249 (MQ=255)                                                                                                                                                                                                                                           aGACTGAGCTGTATCACATTGTAGAAGCGCTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGTGCTACCTGCCGAGCGACCGTGACAAATTCGTCTTCACCGGTGAGTGATACGAAGGCCTTTGAGGTGTGGACGACAGTGACGCAATGTACGAGTCGTGCTGGGACGAGCTGATGCAAGTGCGTGGCGAAGCGAACAAAGTCATTGAGCAAG‑CGCGTGCCTATAAGaacgt  >  2:33408/1‑247 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAG‑CGCGTGCCGACAAGAAAGT  >  NC_000913/24402‑24885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑