Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,340	T→C	D650D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,340	0	T	C	96.4%	165.6 / ‑2.7	57	D650D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (21/33);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCA  >  NC_000913/24096‑24584                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ggTTTATGGCTTCCCTAATGATCTCCACCGCGATGTAAGGCAAAGCGCCGTAACGGCAGGTACTGACCCACGGCGTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGTAACACCGTTTCGCCGCAGGATGTGATGAACAAGCGGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTa                                                                                                                                                                                                                                                 <  2:138596/250‑1 (MQ=255) ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGATACTGACCCACGGCTTTACCGAGGATGGTCAGGGCCGCAAGATGTCTAACTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTAGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCAACTACACCGGGGAACTGACAGTTTCTTACGCAATCCTGAAACGTGCTGCCGACAGCTAt                                                                                                                                                                                                                                                >  1:343906/1‑251 (MQ=255)    tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                                                                                                                                                                                                                                              <  1:200705/250‑1 (MQ=255)     cATGGCTTCCCTAATGATCTCCACCGCGATGAAATGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCTTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCGGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGTCGAGATCCTGAAACGTGCTGCCGACAGCTAtcgtc                                                                                                                                                                                                                                            <  1:251836/251‑1 (MQ=255)         tCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTAt                                                                                                                                                                                                                                        >  1:346054/1‑251 (MQ=255)                  aTGATCTCCACCGCGATGAAAGGCAAAGAGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACCCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGGTATTCTTCGTCTGG‑GGGTGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATACTGAAACGTGCTGCCGGCAGCTATCATCTTATCCgaaaaac                                                                                                                                                                                                                               >  2:235758/1‑245 (MQ=255)                                gATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGAGCTTCctgc                                                                                                                                                                                                                 >  1:371410/1‑251 (MQ=255)                                     aaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCa                                                                                                                                                                                                            <  1:206503/246‑1 (MQ=255)                                       ggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGAGATGAACAAACTGGGCGCGGATATTCTGCGTCTGA‑GGGTGGCATCAACCGACTCCACCGGTGACATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACCGCTATCGTCGTATCCGTAACACCGCGCGCCTCCTGCTGGcca                                                                                                                                                                                                           >  1:45337/4‑248 (MQ=255)                                                    aTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                                                                                                                                             <  1:322366/251‑1 (MQ=255)                                                         cAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACTGTTTTGATcc                                                                                                                                                                                        <  1:132628/251‑1 (MQ=255)                                                                              aCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACGGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATTGTGAAAc                                                                                                                                                                    <  1:106493/250‑1 (MQ=255)                                                                               ccGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑AGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCg                                                                                                                                                                  <  1:227742/251‑1 (MQ=255)                                                                                    gATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCTTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAaga                                                                                                                                                             <  1:266672/251‑1 (MQ=255)                                                                                          aaGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggt                                                                                                                                                       <  1:60163/250‑1 (MQ=255)                                                                                           aGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggtg                                                                                                                                                      <  1:282445/251‑1 (MQ=255)                                                                                             ggCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATggtggt                                                                                                                                                    <  1:145152/251‑1 (MQ=255)                                                                                                                     ggCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCAGAAGAGATGGAGGTACTGGGTCGCTGGGCCGTGGGTtg                                                                                                                            >  2:173640/1‑251 (MQ=255)                                                                                                                                    ccgcAGGATGTGAGTAACAAAAGGGGCGGGGATTTTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCTTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGGAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGAGCCAGCAAAAGATATGGTGAAAGCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGcaca                                                                                                             <  1:54836/251‑1 (MQ=255)                                                                                                                                        cgtttgagaggaACAACCGGGGCGCTGAGATTCTGCG‑CTTTAGGGTGGCATCCACCGACTACACCGGTCAATTGGCCGTTTCTGGCGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTAACCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGaa                                                                                                         <  2:3146/241‑1 (MQ=255)                                                                                                                                           aTGTGATGAACAAACTGGTCGCGGATATTCTGTGTCTGT‑GGGTGGTATCAACCGCCTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTCCCTGCTGGCAAACCTCATCGGTTTTGCTCCCGCATAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGTAAGAc                                                                                                      <  1:74901/251‑1 (MQ=255)                                                                                                                                            tgtgATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGCAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAGGCGGCACAGGAAGAc                                                                                                      >  1:83568/1‑250 (MQ=255)                                                                                                                                             gtgATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACAt                                                                                                    <  1:265887/251‑1 (MQ=255)                                                                                                                                             gtgATGAACAAACTGGGCGCGGATAGTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACAt                                                                                                    <  1:265891/251‑1 (MQ=255)                                                                                                                                                atgaACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGTTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCt                                                                                                 >  1:241046/1‑251 (MQ=255)                                                                                                                                                      aaaCTGGGCGCGGATATTCTGCGTCTGT‑GCGTGGCATCAAACGACTACACCGGTGCAATGGCCGTTTCTGACGAGCTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGc                                                                                           >  1:130035/1‑251 (MQ=255)                                                                                                                                                           tggCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGCTCGCTGGGCCGTAGGTTGTGCGAAAGGGGCACAGGAAGACATCCTCAAGGCGTACg                                                                                      >  2:91138/2‑251 (MQ=255)                                                                                                                                                           gggCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGTTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTAc                                                                                       <  1:61417/250‑1 (MQ=255)                                                                                                                                                              cgcgGATATTCAGCGTCTGT‑GAGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGAGCTGCCGACAGCTATCGTCGTATCCGTAAAACCGCGAGCTTCCTGCTGGCCACCCTGAACGATTTTGATCCAGCAACAGCTATGGTGAAACCGGAAGCGATGGTGGAACTGGCTCGCTGGGCCGTAGGTTGTGCAAACGCGGACCAGGACGCCATCCTCCAGGCgacagcgt                                                                                   >  2:230444/1‑244 (MQ=255)                                                                                                                                                                cgGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGc                                                                                  >  1:153608/1‑250 (MQ=255)                                                                                                                                                                       tCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGAGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGAtt                                                                          >  1:13902/1‑251 (MQ=255)                                                                                                                                                                                gt‑gggtggCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAg                                                                 <  1:168274/251‑1 (MQ=255)                                                                                                                                                                                    ggtgctcgcAACCGACTTCACATGTGAAATGGCCGTTTCTGACGATATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTTATCGGTTTTGATCCATCAAAAGATATGTTGAAACCGGAAGAGATTGTTGTACTGGATCGCTGTGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACTAAGCATACGATTTCCTCGAAGTgg                                                              <  2:371410/243‑1 (MQ=255)                                                                                                                                                                                     gtggCATAAACCGACTACACCGGTGAAATGACCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAACTGAACGGTTTTGATCCAGCAAAAGATCTGGTGAAACCGGACGAGATGGGGGGACTGGATCGCAGGTCCGTAGGTTGTGCGAAAGAGGCAATGGTAGTAATCCTCAAGGCGTACGATGAATACGTGTTCCACGacgaggt                                                             >  2:163732/1‑245 (MQ=255)                                                                                                                                                                                        gCATCAACCTACTACTCCGGTGAAATGGCCGGTTCTGACGAGATCCTGAAACGGGCGTCCGTCAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTTCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGACCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACATGAAGACATCCACAAGGCGTGCGAAGCATACGATTTCCACCAAGTGGTACa                                                          <  2:54612/251‑1 (MQ=255)                                                                                                                                                                                         cATCAACCGACTACGCCGATGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGTATACGATTTCCACGAAGTGGGACAg                                                         <  2:22669/251‑1 (MQ=255)                                                                                                                                                                                          aTCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGc                                                        <  1:101510/251‑1 (MQ=255)                                                                                                                                                                                          aTCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGc                                                        <  1:279759/251‑1 (MQ=255)                                                                                                                                                                                            cAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATCCGATTTCCACGAAGTGGTACAGCg                                                       <  1:97874/250‑1 (MQ=255)                                                                                                                                                                                              aCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCt                                                    >  1:89402/1‑251 (MQ=255)                                                                                                                                                                                               ccGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTg                                                   <  1:229611/251‑1 (MQ=255)                                                                                                                                                                                               ccGACTACACCGGTGAAATGACCGTTTCTGACGAGATCCTGAAACGTGATGCCGACCGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCCAAAGATATGGTAAAACCGGAAGAGATGGTGGTACTGGATCGCTAGGCCGTAGGGTGTGTGGAAGAGGCACAGGAAGCCATCCTAACGGCGTACGGAGCAAACGATTTCCAGGACGTAGTACAGCGtagg                                                   >  2:171151/1‑248 (MQ=255)                                                                                                                                                                                                    tACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAGGTGGTACAGCGTCTGATgcg                                              >  1:49249/1‑251 (MQ=255)                                                                                                                                                                                                     accccGGTGAAATGGCCGTTTCTGACTAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTCCCACGAAGTGGTACAGCGTCTGATgcg                                              <  1:36997/247‑1 (MQ=255)                                                                                                                                                                                                      cacCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCt                                            <  1:259349/251‑1 (MQ=255)                                                                                                                                                                                                       cccGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCtt                                           <  1:340885/250‑1 (MQ=255)                                                                                                                                                                                                        ccGGTGAAATGGCCGTTTCTGACGAGCTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTc                                          >  1:311396/1‑251 (MQ=255)                                                                                                                                                                                                          ggTGAAATGGACGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGAGCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTg                                        <  1:242427/251‑1 (MQ=255)                                                                                                                                                                                                              aaaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTcc                                    <  1:91138/251‑1 (MQ=255)                                                                                                                                                                                                               aaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCg                                   <  1:327700/251‑1 (MQ=255)                                                                                                                                                                                                               aaTGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATACGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCAGTCGGTTGGGCGAAAGGGGAACAGGAAGACATCCTCAAGTCGGACAAAGCATAAGATTTCCCTGCAGTGGAACAGCGTCTGATGCGCTTCTGCTCCg                                   >  1:346415/1‑251 (MQ=255)                                                                                                                                                                                                                    ccGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTgag                              <  1:23698/251‑1 (MQ=255)                                                                                                                                                                                                                                ggaTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTc                  <  2:89402/250‑1 (MQ=255)                                                                                                                                                                                                                                 gaTCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGGTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCt                 >  1:217337/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGAAACGTGCTGCCGACAGCTATCATCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTc            >  1:200433/1‑251 (MQ=255)                                                                                                                                                                                                                                              gctgcCGAAAGCTATCGTCGTATCCGTAACACCGCGCGATTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGAcatca     <  2:327200/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gcCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGGTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGGTGCACTTCTGCTCCGTTGAGATGGGTTCCTTCTAACTCGCcatcatac  >  2:238202/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGT‑GGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTGTGCGAAAGCGGCACAGGAAGACATCCTCAAGGCGTACGAAGCATACGATTTCCACGAAGTGGTACAGCGTCTGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCA  >  NC_000913/24096‑24584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑