Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,217	T→C	N609N (AAT→AAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,217	0	T	C	97.7%	125.8 / ‑4.4	44	N609N (AAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (14/28);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

CCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTG  >  NC_000913/23972‑24462                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ccggatcctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                                                                                                                                                                   <  1:114788/236‑1 (MQ=255)       actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTATTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGAGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATAGTCAGGCCCGCAAGATGTCGAAATCCATCGGCAGAACCGttcagac                                                                                                                                                                                                                                             >  2:70206/10‑246 (MQ=255)                 tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                                                                                                                   >  1:22669/1‑251 (MQ=255)                  aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATGTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                                                                                                                                                                  <  1:358016/251‑1 (MQ=255)                       ttGACTCCGTATCTACCCTCTCTTCTGTTGTTGTCGTGCGTCCGGAAGTTGCCGGTCACGCATCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑aa                                                                                                                                                                                                                             <  1:241201/250‑1 (MQ=255)                                   cTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGcgcg                                                                                                                                                                                                                >  1:3146/1‑251 (MQ=255)                                      cccACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGat                                                                                                                                                                                                             <  1:66719/251‑1 (MQ=255)                                                        aCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggtg                                                                                                                                                                                           >  1:327200/1‑251 (MQ=255)                                                         ccggcgtacggattctgcatgTCACGCAGCTGTCACGTAGCTGGAAGTTTCTGACCCGCGCCGGGTTTGGGTCATGTCTTCCCTAAGGATCGCCACCGTGATGAACGGCAAAGCGCCTGATCGTCAGGTACTGACCCACGGCTTTACCGTGGTTGGGCAGGGCCGCAAGATGTATAATTCCATCGTCAACTTCGTATCGCCGTCGGATGTTATG‑AACAAACTGGGCGCGGATATTCTGCGTCtgtgttggt                                                                                                                                                                                          <  2:89305/231‑6 (MQ=255)                                                           tGCGTCCAGAATTTGCCGGACACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGGTTCGCCGCCGGATGTGAGG‑AACAAACTGGGCGCGGAAATTCTGCGTCTGTGGGTGGc                                                                                                                                                                                         >  2:55871/1‑250 (MQ=255)                                                               tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCCCGGCTTTACCGTGGATGGTAAGGGCAGCAAGATTTCTAAATCCATCGGCAACACCGCTTCGCCGCCGGATTTGATG‑AACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCa                                                                                                                                                                                     >  2:96568/1‑250 (MQ=255)                                                                    aaTTTGCCGGTCACGCTACGGTCATGTATCTGGAAGGTTCTGATCAGCACCGTGGTTGGTGCATGGCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGTATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGAc                                                                                                                                                                               <  2:170413/251‑1 (MQ=255)                                                                       ttGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGTGATG‑ACCAAACTGGGCGCGGATATTCTTCGGCTGTGGGTGGCATCCACCggcca                                                                                                                                                                             >  2:259349/1‑246 (MQ=255)                                                                         gCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTTTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTaca                                                                                                                                                                           <  1:338675/250‑1 (MQ=255)                                                                                               aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                                                                                                                                                        >  2:244889/1‑168 (MQ=255)                                                                                               aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                                                                                                                                                        <  1:244889/168‑1 (MQ=255)                                                                                                tCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggt                                                                                                                                                                                            <  1:356359/210‑1 (MQ=255)                                                                                                   ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGAc                                                                                                                                                    <  1:147757/247‑1 (MQ=255)                                                                                                   ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACgag                                                                                                                                                 <  1:66980/250‑1 (MQ=255)                                                                                                       ggTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGCTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCCGGACG‑GGTGTAAACAACTGGCCGCGGATATTCGTCGTCTGTGGGGTGAATCCACCAGCCACACCGGAGAAATGGCCGTTTCTGatggggaccc                                                                                                                                            >  2:100232/1‑242 (MQ=255)                                                                                                             gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATAGCCGTTTCTGACGAGATCCTGAAAc                                                                                                                                       <  1:352326/250‑1 (MQ=255)                                                                                                             acccagcacCGGGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAATGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTGCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGTGCGCGGATATTCTGCGTCTGTGGGTGGCATCACCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAAc                                                                                                                                       <  1:83552/244‑1 (MQ=255)                                                                                                               ccagcaacgtggttGGTTCATGTCTTCCCTAATGATCTCAACCGCGATGACAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGGTGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGGTGGGCTG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCCTCAAACAACTACAACGGTGAAATAGCCGTTTCAGACGAGGACCTGaacaa                                                                                                                                      >  2:310667/14‑246 (MQ=255)                                                                                                                   cacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgctgc                                                                                                                                <  1:143539/251‑1 (MQ=255)                                                                                                                    accgtggttGGTTCAGTTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAACGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACTCCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGcc                                                                                                                               <  1:8169/243‑1 (MQ=255)                                                                                                                     ccgtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCg                                                                                                                              <  1:55871/244‑1 (MQ=255)                                                                                                                             ggTTTATGGCTTCCCTAATGATCTCCACCGCGATGTAAGGCAAAGCGCCGTAACGGCAGGTACTGACCCACGGCGTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGTAACACCGTTTCGCCGCAGGATGTGATG‑AACAAGCGGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTa                                                                                                                       <  2:138596/250‑1 (MQ=255)                                                                                                                             ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGATACTGACCCACGGCTTTACCGAGGATGGTCAGGGCCGCAAGATGTCTAACTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTAGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCAACTACACCGGGGAACTGACAGTTTCTTACGCAATCCTGAAACGTGCTGCCGACAGCTAt                                                                                                                      >  1:343906/1‑251 (MQ=255)                                                                                                                                tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                                                                                                                    <  1:200705/250‑1 (MQ=255)                                                                                                                                 cATGGCTTCCCTAATGATCTCCACCGCGATGAAATGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCTTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCGGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGTCGAGATCCTGAAACGTGCTGCCGACAGCTAtcgtc                                                                                                                  <  1:251836/251‑1 (MQ=255)                                                                                                                                     tCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTAt                                                                                                              >  1:346054/1‑251 (MQ=255)                                                                                                                                              aTGATCTCCACCGCGATGAAAGGCAAAGAGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACCCCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGGTATTCTTCGTCTGGGGGTGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATACTGAAACGTGCTGCCGGCAGCTATCATCTTATCCgaaaaac                                                                                                     >  2:235758/1‑245 (MQ=255)                                                                                                                                                            gATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGAGCTTCctgc                                                                                       >  1:371410/1‑251 (MQ=255)                                                                                                                                                                 aaggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCa                                                                                  <  1:206503/246‑1 (MQ=255)                                                                                                                                                                   ggcaaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGAGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGAGGGTGGCATCAACCGACTCCACCGGTGACATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACCGCTATCGTCGTATCCGTAACACCGCGCGCCTCCTGCTGGcca                                                                                 >  1:45337/4‑248 (MQ=255)                                                                                                                                                                                aTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGtttt                                                                   <  1:322366/251‑1 (MQ=255)                                                                                                                                                                                     cAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACTGTTTTGATcc                                                              <  1:132628/251‑1 (MQ=255)                                                                                                                                                                                                          aCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACGGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATTGTGAAAc                                          <  1:106493/250‑1 (MQ=255)                                                                                                                                                                                                           ccGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTAGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCg                                        <  1:227742/251‑1 (MQ=255)                                                                                                                                                                                                                gATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCTTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAaga                                   <  1:266672/251‑1 (MQ=255)                                                                                                                                                                                                                      aaGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggt                             <  1:60163/250‑1 (MQ=255)                                                                                                                                                                                                                       aGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggtg                            <  1:282445/251‑1 (MQ=255)                                                                                                                                                                                                                         ggCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATggtggt                          <  1:145152/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ggCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCAGAAGAGATGGAGGTACTGGGTCGCTGGGCCGTGGGTtg  >  2:173640/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTG  >  NC_000913/23972‑24462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑