Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,133	G→A	K581K (AAG→AAA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,133	0	G	A	98.0%	133.2 / ‑4.5	51	K581K (AAG→AAA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (22/26);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

GAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGC  >  NC_000913/23891‑24377                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gaagttaacggCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCTACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                                                                <  2:118720/241‑1 (MQ=255) gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                                                                <  1:310494/241‑1 (MQ=255)          ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAGCAGTATGTGAAAGTGCCGGATACTCTCGACGAATGGTTTGACTCCGGATCTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAACTCCAGCGCTATGAAAGGCAAAGCGCCGTATCg                                                                                                                                                                                                                                      >  2:266672/1‑251 (MQ=255)                          gggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTCTCTGGAAGGATCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACAGCGATGAAAGGCAAAGCGCCGTCTCGTCAGGCACTGAACCAc                                                                                                                                                                                                                      >  2:279759/1‑251 (MQ=255)                             atcgcGATGCGAAAGAGATCCTCGGCGACGAAGCTGGCCTGTTTGTGAAAGTGCCGGATACTCTCGGCGTATGGTTTGACTGCGGATCTACCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAGAGCGCCGTATCGTCAGGTACTGTCCCACGGc                                                                                                                                                                                                                   <  2:195622/247‑1 (MQ=255)                                 cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTa                                                                                                                                                                                                               <  1:81009/251‑1 (MQ=255)                                   atacGAAAGAGATCCTCGGCGACGATGCTGACCAGTATTGGAAAGGTCCGGATACTCTTGACGTATGGTTTGACGCCGGATCTACCCACTCTGATGTTGTTGACGTGCGTCCTGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGATCAGCATCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCTCCGCGATGAAAGGCAAAGCGCCGTTGCGTCAGGTACTGACTTACGGTTTTAcc                                                                                                                                                                                                             <  2:369161/248‑1 (MQ=255)                                   aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAcc                                                                                                                                                                                                             <  1:88762/251‑1 (MQ=255)                                   aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGCCCCCCGGCTTTAcc                                                                                                                                                                                                             >  2:143539/1‑251 (MQ=255)                                      cGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAATCGCCGTATCGTCAGGTACTTACCCACGGCTTTACCGTg                                                                                                                                                                                                          >  2:145152/1‑251 (MQ=255)                                                ccgcGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAgg                                                                                                                                                                                                <  1:167965/248‑1 (MQ=255)                                                ccTCGGCGACGAAGCTGACCAGTATGTGAAAGCGCCGGATACTCTCGACGTATGGCTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCTCCGTAGCGTCATGGAATGCCCCACGGCTTTACCGTTGATGGTccgg                                                                                                                                                                                                >  2:372466/1‑248 (MQ=255)                                                   cGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                                                                                                                                                                                             <  1:361058/251‑1 (MQ=255)                                                    ggCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACAGTGGATGGTCAGGGCCg                                                                                                                                                                                            >  2:101510/1‑251 (MQ=255)                                                           aaGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATg                                                                                                                                                                                     >  1:73456/1‑251 (MQ=255)                                                                              agtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                                                                   >  1:54612/20‑249 (MQ=255)                                                                                  ccggatcctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                                                                              <  1:114788/236‑1 (MQ=255)                                                                                        actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTATTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGAGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATAGTCAGGCCCGCAAGATGTCGAAATCCATCGGCAGAACCGttcagac                                                                                                                                                        >  2:70206/10‑246 (MQ=255)                                                                                                  tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                              >  1:22669/1‑251 (MQ=255)                                                                                                   aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATGTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                                                                             <  1:358016/251‑1 (MQ=255)                                                                                                        ttGACTCCGTATCTACCCTCTCTTCTGTTGTTGTCGTGCGTCCGGAAGTTGCCGGTCACGCATCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑aa                                                                                                                                        <  1:241201/250‑1 (MQ=255)                                                                                                                    cTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGcgcg                                                                                                                           >  1:3146/1‑251 (MQ=255)                                                                                                                       cccACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGat                                                                                                                        <  1:66719/251‑1 (MQ=255)                                                                                                                           ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                                                                                                                                                                                            >  1:78652/1‑180 (MQ=255)                                                                                                                           ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGTCGTATCGGCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcag                                                                                                                                                                                            <  2:78652/180‑3 (MQ=255)                                                                                                                                         aCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggtg                                                                                                      >  1:327200/1‑251 (MQ=255)                                                                                                                                          ccggcgtacggattctgcatgTCACGCAGCTGTCACGTAGCTGGAAGTTTCTGACCCGCGCCGGGTTTGGGTCATGTCTTCCCTAAGGATCGCCACCGTGATGAACGGCAAAGCGCCTGATCGTCAGGTACTGACCCACGGCTTTACCGTGGTTGGGCAGGGCCGCAAGATGTATAATTCCATCGTCAACTTCGTATCGCCGTCGGATGTTATG‑AACAAACTGGGCGCGGATATTCTGCGTCtgtgttggt                                                                                                     <  2:89305/231‑6 (MQ=255)                                                                                                                                            tGCGTCCAGAATTTGCCGGACACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGGTTCGCCGCCGGATGTGAGG‑AACAAACTGGGCGCGGAAATTCTGCGTCTGTGGGTGGc                                                                                                    >  2:55871/1‑250 (MQ=255)                                                                                                                                                tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCCCGGCTTTACCGTGGATGGTAAGGGCAGCAAGATTTCTAAATCCATCGGCAACACCGCTTCGCCGCCGGATTTGATG‑AACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCa                                                                                                >  2:96568/1‑250 (MQ=255)                                                                                                                                                     aaTTTGCCGGTCACGCTACGGTCATGTATCTGGAAGGTTCTGATCAGCACCGTGGTTGGTGCATGGCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGTATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGAc                                                                                          <  2:170413/251‑1 (MQ=255)                                                                                                                                                        ttGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGTGATG‑ACCAAACTGGGCGCGGATATTCTTCGGCTGTGGGTGGCATCCACCggcca                                                                                        >  2:259349/1‑246 (MQ=255)                                                                                                                                                          gCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTTTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTaca                                                                                      <  1:338675/250‑1 (MQ=255)                                                                                                                                                                                aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                                                                   <  1:244889/168‑1 (MQ=255)                                                                                                                                                                                aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                                                                   >  2:244889/1‑168 (MQ=255)                                                                                                                                                                                 tCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggt                                                                                                       <  1:356359/210‑1 (MQ=255)                                                                                                                                                                                    ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGAc                                                               <  1:147757/247‑1 (MQ=255)                                                                                                                                                                                    ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACgag                                                            <  1:66980/250‑1 (MQ=255)                                                                                                                                                                                        ggTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGCTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCCGGACG‑GGTGTAAACAACTGGCCGCGGATATTCGTCGTCTGTGGGGTGAATCCACCAGCCACACCGGAGAAATGGCCGTTTCTGatggggaccc                                                       >  2:100232/1‑242 (MQ=255)                                                                                                                                                                                              gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATAGCCGTTTCTGACGAGATCCTGAAAc                                                  <  1:352326/250‑1 (MQ=255)                                                                                                                                                                                              acccagcacCGGGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAATGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTGCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGTGCGCGGATATTCTGCGTCTGTGGGTGGCATCACCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAAc                                                  <  1:83552/244‑1 (MQ=255)                                                                                                                                                                                                ccagcaacgtggttGGTTCATGTCTTCCCTAATGATCTCAACCGCGATGACAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGGTGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGGTGGGCTG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCCTCAAACAACTACAACGGTGAAATAGCCGTTTCAGACGAGGACCTGaacaa                                                 >  2:310667/14‑246 (MQ=255)                                                                                                                                                                                                    cacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgctgc                                           <  1:143539/251‑1 (MQ=255)                                                                                                                                                                                                     accgtggttGGTTCAGTTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAACGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACTCCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGcc                                          <  1:8169/243‑1 (MQ=255)                                                                                                                                                                                                      ccgtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCg                                         <  1:55871/244‑1 (MQ=255)                                                                                                                                                                                                              ggTTTATGGCTTCCCTAATGATCTCCACCGCGATGTAAGGCAAAGCGCCGTAACGGCAGGTACTGACCCACGGCGTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGTAACACCGTTTCGCCGCAGGATGTGATG‑AACAAGCGGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTa                                  <  2:138596/250‑1 (MQ=255)                                                                                                                                                                                                              ggTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGATACTGACCCACGGCTTTACCGAGGATGGTCAGGGCCGCAAGATGTCTAACTCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTAGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCAACTACACCGGGGAACTGACAGTTTCTTACGCAATCCTGAAACGTGCTGCCGACAGCTAt                                 >  1:343906/1‑251 (MQ=255)                                                                                                                                                                                                                 tCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcg                               <  1:200705/250‑1 (MQ=255)                                                                                                                                                                                                                  cATGGCTTCCCTAATGATCTCCACCGCGATGAAATGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCTTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCGGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGTCGAGATCCTGAAACGTGCTGCCGACAGCTAtcgtc                             <  1:251836/251‑1 (MQ=255)                                                                                                                                                                                                                      tCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTAt                         >  1:346054/1‑251 (MQ=255)                                                                                                                                                                                                                               aTGATCTCCACCGCGATGAAAGGCAAAGAGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCAATCGGCAACCCCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGGTATTCTTCGTCTGGGGGTGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATACTGAAACGTGCTGCCGGCAGCTATCATCTTATCCgaaaaac                >  2:235758/1‑245 (MQ=255)                                                                                                                                                                                                                                             gATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGAGCTTCctgc  >  1:371410/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGC  >  NC_000913/23891‑24377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑