Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,094	C→T	G568G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,094	0	C	T	90.4%	124.8 / 4.6	53	G568G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (20/27);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

GCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGC  >  NC_000913/23851‑24336                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gcgTACCATTGAAATGATGGAAGAAGTGGAAAAAAGAGTTGAAGTAGATGGTATCAAGGAGTGGTGGGATATAGATGAGAAAGAGATAATAGGAGAAGAAGATGATCAGTAAGTGAAAGTGCAGGAAAAATTGGATGTATGGTTTGAATACGGATATAAAAAATATTATGTTGTTGACGTGCGTACGGAATTTGCAGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGAGGCTGGTTCa                                                                                                                                                                                                                                              <  1:251117/251‑1 (MQ=255)           gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                                                                                                                                                    <  1:99796/251‑1 (MQ=255)               tGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGAAGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATg                                                                                                                                                                                                                                >  1:229218/1‑251 (MQ=255)                gATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGTCATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGa                                                                                                                                                                                                                               <  1:16331/251‑1 (MQ=255)                   ggAAGAAGTGGCAAAACGCGTTGAATTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGATGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtct                                                                                                                                                                                                                            <  1:242797/251‑1 (MQ=255)                       gaagTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTTATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTACGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGGAGGTTCTGACCAACAACGCGGCTGGTTCATGTCTTCCCTAATGATCTCCAc                                                                                                                                                                                                                        <  1:156967/251‑1 (MQ=255)                                  aCGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa                                                                                                                                                                                                               >  1:187875/1‑249 (MQ=255)                                   cgcgTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaaagg                                                                                                                                                                                                            <  1:111146/251‑4 (MQ=255)                                    gcgTTGAAGTTAACGGAATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCCACGAAGCTGACCAGTATGTAAAAGTGCCGGATACTCTAGAAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGCAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGTTCTCCACCGCGCTGaaaggc                                                                                                                                                                                                           >  1:377387/1‑247 (MQ=255)                                     cgTTGAAGTTAACGGCATCCAGGTGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACTAAGCTGACTAGTATGTGAAAGTGCCTGATACTCTCGACGTATGGTTTGGCTTCGGATCTACCCTCTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCTGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAGCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTGCACCGCGATGaaaggca                                                                                                                                                                                                          <  1:18002/251‑6 (MQ=255)                                         gaagttaacggCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCTACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                       <  2:118720/241‑1 (MQ=255)                                         gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                       <  1:310494/241‑1 (MQ=255)                                         gaagttaacgacATCCAGGCGCGGTGGGATCTCGACGCGAATGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGTGCCGGATACTCTCGGCGTATGTTTTGACTCCGGATCTACCCAATCTTCTGGGGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGGCATGTATCTGGCAGGTTCTGACCAGCACCGTGAGTGGAGCATGTCTTCCCTCATGATCTCCACCTCGAttaaaggcaaagg                                                                                                                                                                                                      >  2:97874/12‑239 (MQ=255)                                             taaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGCGAAAAAGCTGCACAGTATGTGAAAGTGCCGAAGACTCTCGACGTATGGATTGACTCCGGATCTACCAACTCTTCAGGTGTTTCCGTGCGTCCGGACATTGGCGGTCACGCCACCGACATGTATATGGAAGGTTCTGACCCACACAGAGGTAGGTTCAAGTCTTACCTAATGAACTCCCCCGCGAGGaaagacagagcgccg                                                                                                                                                                                                  >  2:311613/6‑238 (MQ=255)                                                  ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAGCAGTATGTGAAAGTGCCGGATACTCTCGACGAATGGTTTGACTCCGGATCTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAACTCCAGCGCTATGAAAGGCAAAGCGCCGTATCg                                                                                                                                                                                             >  2:266672/1‑251 (MQ=255)                                                                  gggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTCTCTGGAAGGATCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACAGCGATGAAAGGCAAAGCGCCGTCTCGTCAGGCACTGAACCAc                                                                                                                                                                             >  2:279759/1‑251 (MQ=255)                                                                     atcgcGATGCGAAAGAGATCCTCGGCGACGAAGCTGGCCTGTTTGTGAAAGTGCCGGATACTCTCGGCGTATGGTTTGACTGCGGATCTACCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAGAGCGCCGTATCGTCAGGTACTGTCCCACGGc                                                                                                                                                                          <  2:195622/247‑1 (MQ=255)                                                                         cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTa                                                                                                                                                                      <  1:81009/251‑1 (MQ=255)                                                                           atacGAAAGAGATCCTCGGCGACGATGCTGACCAGTATTGGAAAGGTCCGGATACTCTTGACGTATGGTTTGACGCCGGATCTACCCACTCTGATGTTGTTGACGTGCGTCCTGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGATCAGCATCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCTCCGCGATGAAAGGCAAAGCGCCGTTGCGTCAGGTACTGACTTACGGTTTTAcc                                                                                                                                                                    <  2:369161/248‑1 (MQ=255)                                                                           aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAcc                                                                                                                                                                    <  1:88762/251‑1 (MQ=255)                                                                           aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGCCCCCCGGCTTTAcc                                                                                                                                                                    >  2:143539/1‑251 (MQ=255)                                                                              cGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAATCGCCGTATCGTCAGGTACTTACCCACGGCTTTACCGTg                                                                                                                                                                 >  2:145152/1‑251 (MQ=255)                                                                                        ccgcGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAgg                                                                                                                                                       <  1:167965/248‑1 (MQ=255)                                                                                        ccTCGGCGACGAAGCTGACCAGTATGTGAAAGCGCCGGATACTCTCGACGTATGGCTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCTCCGTAGCGTCATGGAATGCCCCACGGCTTTACCGTTGATGGTccgg                                                                                                                                                       >  2:372466/1‑248 (MQ=255)                                                                                           cGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                                                                                                                                                    <  1:361058/251‑1 (MQ=255)                                                                                            ggCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACAGTGGATGGTCAGGGCCg                                                                                                                                                   >  2:101510/1‑251 (MQ=255)                                                                                                   aaGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATg                                                                                                                                            >  1:73456/1‑251 (MQ=255)                                                                                                                      agtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                          >  1:54612/20‑249 (MQ=255)                                                                                                                          ccggatcctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                                     <  1:114788/236‑1 (MQ=255)                                                                                                                                actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTATTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGAGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATAGTCAGGCCCGCAAGATGTCGAAATCCATCGGCAGAACCGttcagac                                                                                                               >  2:70206/10‑246 (MQ=255)                                                                                                                                          tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                     >  1:22669/1‑251 (MQ=255)                                                                                                                                           aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATGTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                                    <  1:358016/251‑1 (MQ=255)                                                                                                                                                ttGACTCCGTATCTACCCTCTCTTCTGTTGTTGTCGTGCGTCCGGAAGTTGCCGGTCACGCATCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑aa                                                                                               <  1:241201/250‑1 (MQ=255)                                                                                                                                                            cTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGcgcg                                                                                  >  1:3146/1‑251 (MQ=255)                                                                                                                                                               cccACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGat                                                                               <  1:66719/251‑1 (MQ=255)                                                                                                                                                                   ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                                                                                                                                                   >  1:78652/1‑180 (MQ=255)                                                                                                                                                                   ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGTCGTATCGGCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcag                                                                                                                                                   <  2:78652/180‑3 (MQ=255)                                                                                                                                                                                 aCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggtg                                                             >  1:327200/1‑251 (MQ=255)                                                                                                                                                                                  ccggcgtacggattctgcatgTCACGCAGCTGTCACGTAGCTGGAAGTTTCTGACCCGCGCCGGGTTTGGGTCATGTCTTCCCTAAGGATCGCCACCGTGATGAACGGCAAAGCGCCTGATCGTCAGGTACTGACCCACGGCTTTACCGTGGTTGGGCAGGGCCGCAAGATGTATAATTCCATCGTCAACTTCGTATCGCCGTCGGATGTTATG‑AACAAACTGGGCGCGGATATTCTGCGTCtgtgttggt                                                            <  2:89305/231‑6 (MQ=255)                                                                                                                                                                                    tGCGTCCAGAATTTGCCGGACACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGGTTCGCCGCCGGATGTGAGG‑AACAAACTGGGCGCGGAAATTCTGCGTCTGTGGGTGGc                                                           >  2:55871/1‑250 (MQ=255)                                                                                                                                                                                        tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCCCGGCTTTACCGTGGATGGTAAGGGCAGCAAGATTTCTAAATCCATCGGCAACACCGCTTCGCCGCCGGATTTGATG‑AACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCa                                                       >  2:96568/1‑250 (MQ=255)                                                                                                                                                                                             aaTTTGCCGGTCACGCTACGGTCATGTATCTGGAAGGTTCTGATCAGCACCGTGGTTGGTGCATGGCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGTATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGAc                                                 <  2:170413/251‑1 (MQ=255)                                                                                                                                                                                                ttGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGTGATG‑ACCAAACTGGGCGCGGATATTCTTCGGCTGTGGGTGGCATCCACCggcca                                               >  2:259349/1‑246 (MQ=255)                                                                                                                                                                                                  gCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTTTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTaca                                             <  1:338675/250‑1 (MQ=255)                                                                                                                                                                                                                        aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                          >  2:244889/1‑168 (MQ=255)                                                                                                                                                                                                                        aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                          <  1:244889/168‑1 (MQ=255)                                                                                                                                                                                                                         tCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggt                                                              <  1:356359/210‑1 (MQ=255)                                                                                                                                                                                                                            ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGAc                      <  1:147757/247‑1 (MQ=255)                                                                                                                                                                                                                            ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACgag                   <  1:66980/250‑1 (MQ=255)                                                                                                                                                                                                                                ggTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGCTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCCGGACG‑GGTGTAAACAACTGGCCGCGGATATTCGTCGTCTGTGGGGTGAATCCACCAGCCACACCGGAGAAATGGCCGTTTCTGatggggaccc              >  2:100232/1‑242 (MQ=255)                                                                                                                                                                                                                                      gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATAGCCGTTTCTGACGAGATCCTGAAAc         <  1:352326/250‑1 (MQ=255)                                                                                                                                                                                                                                      acccagcacCGGGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAATGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTGCCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGTGCGCGGATATTCTGCGTCTGTGGGTGGCATCACCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAAc         <  1:83552/244‑1 (MQ=255)                                                                                                                                                                                                                                            cacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtgctgc  <  1:143539/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGC  >  NC_000913/23851‑24336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑