Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,085	A→G	Q565Q (CAA→CAG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,085	0	A	G	89.1%	123.8 / 5.5	55	Q565Q (CAA→CAG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (21/28);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

AGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAAC  >  NC_000913/23841‑24329                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      agctgcatccgtgggacctcggagtGATGGAAGAATTGGCAAAATGCGCTGAAGGTAACGGCATCCAGGCTGGGTGTGAGCTCGCTGCGAAAGAGTTCCTCGTCGACGAAGCAGACCAGTATGTGAAAGTGCCGGAGACTCTCGACGTATTGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGAAGTGCGTCCGGAATTTGCCGTTCACGCAGCGGACATGTATCTGGAAGGTCCTGACCAGCACcgt                                                                                                                                                                                                                                                 <  2:305355/227‑2 (MQ=255)  gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg                                                                                                                                                                                                                                                <  1:312788/251‑3 (MQ=255)    tGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggt                                                                                                                                                                                                                                              >  1:170413/1‑247 (MQ=255)           gcgTACCATTGAAATGATGGAAGAAGTGGAAAAAAGAGTTGAAGTAGATGGTATCAAGGAGTGGTGGGATATAGATGAGAAAGAGATAATAGGAGAAGAAGATGATCAGTAAGTGAAAGTGCAGGAAAAATTGGATGTATGGTTTGAATACGGATATAAAAAATATTATGTTGTTGACGTGCGTACGGAATTTGCAGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGAGGCTGGTTCa                                                                                                                                                                                                                                       <  1:251117/251‑1 (MQ=255)            cgaaacctcgAACAGATGGAAGAAGTGGGAAAACGCTGTGAAGTTAACGGCATCCAGGCGTGGCGGGATCTCGATGCGAAATAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGGATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCTGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtttgttcga                                                                                                                                                                                                                                      <  2:13978/242‑13 (MQ=255)                     gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                                                                                                                                                                                                                             <  1:99796/251‑1 (MQ=255)                         tGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGAAGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATg                                                                                                                                                                                                                         >  1:229218/1‑251 (MQ=255)                          gATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGTCATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGa                                                                                                                                                                                                                        <  1:16331/251‑1 (MQ=255)                             ggAAGAAGTGGCAAAACGCGTTGAATTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGATGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtct                                                                                                                                                                                                                     <  1:242797/251‑1 (MQ=255)                                 gaagTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTTATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTACGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGGAGGTTCTGACCAACAACGCGGCTGGTTCATGTCTTCCCTAATGATCTCCAc                                                                                                                                                                                                                 <  1:156967/251‑1 (MQ=255)                                            aCGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa                                                                                                                                                                                                        >  1:187875/1‑249 (MQ=255)                                             cgcgTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaaagg                                                                                                                                                                                                     <  1:111146/251‑4 (MQ=255)                                              gcgTTGAAGTTAACGGAATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCCACGAAGCTGACCAGTATGTAAAAGTGCCGGATACTCTAGAAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGCAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGTTCTCCACCGCGCTGaaaggc                                                                                                                                                                                                    >  1:377387/1‑247 (MQ=255)                                               cgTTGAAGTTAACGGCATCCAGGTGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACTAAGCTGACTAGTATGTGAAAGTGCCTGATACTCTCGACGTATGGTTTGGCTTCGGATCTACCCTCTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCTGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAGCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTGCACCGCGATGaaaggca                                                                                                                                                                                                   <  1:18002/251‑6 (MQ=255)                                                   gaagttaacggCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCTACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                <  2:118720/241‑1 (MQ=255)                                                   gaagttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAg                                                                                                                                                                                                <  1:310494/241‑1 (MQ=255)                                                   gaagttaacgacATCCAGGCGCGGTGGGATCTCGACGCGAATGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGTGCCGGATACTCTCGGCGTATGTTTTGACTCCGGATCTACCCAATCTTCTGGGGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGGCATGTATCTGGCAGGTTCTGACCAGCACCGTGAGTGGAGCATGTCTTCCCTCATGATCTCCACCTCGAttaaaggcaaagg                                                                                                                                                                                               >  2:97874/12‑239 (MQ=255)                                                       taaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGCGAAAAAGCTGCACAGTATGTGAAAGTGCCGAAGACTCTCGACGTATGGATTGACTCCGGATCTACCAACTCTTCAGGTGTTTCCGTGCGTCCGGACATTGGCGGTCACGCCACCGACATGTATATGGAAGGTTCTGACCCACACAGAGGTAGGTTCAAGTCTTACCTAATGAACTCCCCCGCGAGGaaagacagagcgccg                                                                                                                                                                                           >  2:311613/6‑238 (MQ=255)                                                            ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGAGCAGTATGTGAAAGTGCCGGATACTCTCGACGAATGGTTTGACTCCGGATCTACCAACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGAAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAACTCCAGCGCTATGAAAGGCAAAGCGCCGTATCg                                                                                                                                                                                      >  2:266672/1‑251 (MQ=255)                                                                            gggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTCTCTGGAAGGATCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACAGCGATGAAAGGCAAAGCGCCGTCTCGTCAGGCACTGAACCAc                                                                                                                                                                      >  2:279759/1‑251 (MQ=255)                                                                               atcgcGATGCGAAAGAGATCCTCGGCGACGAAGCTGGCCTGTTTGTGAAAGTGCCGGATACTCTCGGCGTATGGTTTGACTGCGGATCTACCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAGAGCGCCGTATCGTCAGGTACTGTCCCACGGc                                                                                                                                                                   <  2:195622/247‑1 (MQ=255)                                                                                   cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTa                                                                                                                                                               <  1:81009/251‑1 (MQ=255)                                                                                     atacGAAAGAGATCCTCGGCGACGATGCTGACCAGTATTGGAAAGGTCCGGATACTCTTGACGTATGGTTTGACGCCGGATCTACCCACTCTGATGTTGTTGACGTGCGTCCTGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGATCAGCATCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCTCCGCGATGAAAGGCAAAGCGCCGTTGCGTCAGGTACTGACTTACGGTTTTAcc                                                                                                                                                             <  2:369161/248‑1 (MQ=255)                                                                                     aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAcc                                                                                                                                                             <  1:88762/251‑1 (MQ=255)                                                                                     aTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGGACTGCCCCCCGGCTTTAcc                                                                                                                                                             >  2:143539/1‑251 (MQ=255)                                                                                        cGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAATCGCCGTATCGTCAGGTACTTACCCACGGCTTTACCGTg                                                                                                                                                          >  2:145152/1‑251 (MQ=255)                                                                                                  ccgcGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAgg                                                                                                                                                <  1:167965/248‑1 (MQ=255)                                                                                                  ccTCGGCGACGAAGCTGACCAGTATGTGAAAGCGCCGGATACTCTCGACGTATGGCTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCAAGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCTCCGTAGCGTCATGGAATGCCCCACGGCTTTACCGTTGATGGTccgg                                                                                                                                                >  2:372466/1‑248 (MQ=255)                                                                                                     cGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                                                                                                                                             <  1:361058/251‑1 (MQ=255)                                                                                                      ggCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACAGTGGATGGTCAGGGCCg                                                                                                                                            >  2:101510/1‑251 (MQ=255)                                                                                                             aaGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATg                                                                                                                                     >  1:73456/1‑251 (MQ=255)                                                                                                                                agtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                   >  1:54612/20‑249 (MQ=255)                                                                                                                                    ccggatcctctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGt                                                                                                              <  1:114788/236‑1 (MQ=255)                                                                                                                                          actctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTATTGTTGACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGAGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATAGTCAGGCCCGCAAGATGTCGAAATCCATCGGCAGAACCGttcagac                                                                                                        >  2:70206/10‑246 (MQ=255)                                                                                                                                                    tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                              >  1:22669/1‑251 (MQ=255)                                                                                                                                                     aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATGTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                             <  1:358016/251‑1 (MQ=255)                                                                                                                                                          ttGACTCCGTATCTACCCTCTCTTCTGTTGTTGTCGTGCGTCCGGAAGTTGCCGGTCACGCATCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑aa                                                                                        <  1:241201/250‑1 (MQ=255)                                                                                                                                                                      cTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGcgcg                                                                           >  1:3146/1‑251 (MQ=255)                                                                                                                                                                         cccACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGat                                                                        <  1:66719/251‑1 (MQ=255)                                                                                                                                                                             ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                                                                                                                                            >  1:78652/1‑180 (MQ=255)                                                                                                                                                                             ctctTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGTCGTATCGGCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcag                                                                                                                                            <  2:78652/180‑3 (MQ=255)                                                                                                                                                                                           aCGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggtg                                                      >  1:327200/1‑251 (MQ=255)                                                                                                                                                                                            ccggcgtacggattctgcatgTCACGCAGCTGTCACGTAGCTGGAAGTTTCTGACCCGCGCCGGGTTTGGGTCATGTCTTCCCTAAGGATCGCCACCGTGATGAACGGCAAAGCGCCTGATCGTCAGGTACTGACCCACGGCTTTACCGTGGTTGGGCAGGGCCGCAAGATGTATAATTCCATCGTCAACTTCGTATCGCCGTCGGATGTTATG‑AACAAACTGGGCGCGGATATTCTGCGTCtgtgttggt                                                     <  2:89305/231‑6 (MQ=255)                                                                                                                                                                                              tGCGTCCAGAATTTGCCGGACACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCAACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGGTTCGCCGCCGGATGTGAGG‑AACAAACTGGGCGCGGAAATTCTGCGTCTGTGGGTGGc                                                    >  2:55871/1‑250 (MQ=255)                                                                                                                                                                                                  tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCCCGGCTTTACCGTGGATGGTAAGGGCAGCAAGATTTCTAAATCCATCGGCAACACCGCTTCGCCGCCGGATTTGATG‑AACAAAATGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCa                                                >  2:96568/1‑250 (MQ=255)                                                                                                                                                                                                       aaTTTGCCGGTCACGCTACGGTCATGTATCTGGAAGGTTCTGATCAGCACCGTGGTTGGTGCATGGCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGTATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGAc                                          <  2:170413/251‑1 (MQ=255)                                                                                                                                                                                                          ttGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGGCTAAATCCATCGGCAACACCGTTTCGCCGCAGGCTGTGATG‑ACCAAACTGGGCGCGGATATTCTTCGGCTGTGGGTGGCATCCACCggcca                                        >  2:259349/1‑246 (MQ=255)                                                                                                                                                                                                            gCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTTTCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTaca                                      <  1:338675/250‑1 (MQ=255)                                                                                                                                                                                                                                  aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                   >  2:244889/1‑168 (MQ=255)                                                                                                                                                                                                                                  aTCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAgg                                                                                                   <  1:244889/168‑1 (MQ=255)                                                                                                                                                                                                                                   tCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTgtgggt                                                       <  1:356359/210‑1 (MQ=255)                                                                                                                                                                                                                                      ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGAc               <  1:147757/247‑1 (MQ=255)                                                                                                                                                                                                                                      ggAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACgag            <  1:66980/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGCTCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACACCGTTTCGCCGCCGGACG‑GGTGTAAACAACTGGCCGCGGATATTCGTCGTCTGTGGGGTGAATCCACCAGCCACACCGGAGAAATGGCCGTTTCTGatggggaccc       >  2:100232/1‑242 (MQ=255)                                                                                                                                                                                                                                                gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATAGCCGTTTCTGACGAGATCCTGAAAc  <  1:352326/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATG‑AACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAAC  >  NC_000913/23841‑24329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑