Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108	A→T	Q591L (CAG→CTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108	0	A	T	89.7%	104.8 / 0.9	39	Q591L (CAG→CTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (19/16);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGA  >  NC_000913/1863‑2354                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGtt                                                                                                                                                                                                                                                     <  1:376086/249‑1 (MQ=255) gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTGCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGtt                                                                                                                                                                                                                                                     <  1:171287/249‑1 (MQ=255)      ccAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGtcat                                                                                                                                                                                                                                              <  1:159096/251‑4 (MQ=255)             gcATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGcggcgg                                                                                                                                                                                                                                       <  1:328530/250‑1 (MQ=255)                      aaaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAAGCCAAAACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGGAAAATcgc                                                                                                                                                                                                                              >  1:3434/2‑251 (MQ=255)                             gacatcgggCAGGAAGAACGGGCGCATGCCAAAGAGCGTTTTAACCTCTGGTCATTAATTTGCCACGTTAACTAATATCATCTGCTGAACCCGGTCAGTGGTGACTGCACTTCCAGCCAGGCAGTGGCGGAGCAATATGCCGACTTCTTGCGCTAATGTTTCCACGTTGGCCCGCCGAACAAAAAGGCCAACACCGCGTCGATGTATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTaa                                                                                                                                                                                                                       <  2:338677/244‑1 (MQ=255)                             gAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTaa                                                                                                                                                                                                                       <  1:51367/251‑1 (MQ=255)                                            gaaCTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGacac                                                                                                                                                                                                        >  1:204573/1‑251 (MQ=255)                                                    gcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGAGAAGGTTTCCACGGTGTTACGCCGAACAAAAAGGCCCAAACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTTTATGACACCAACGTTg                                                                                                                                                                                                >  1:333450/1‑251 (MQ=255)                                                           aaaGAGCCGTTTAATCGCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTgg                                                                                                                                                                                         <  2:163939/251‑1 (MQ=255)                                                             agagCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGCACAATAAGTCGGCTTCTTGCGCGAAGGTTTCCACGTTGTCACGACGGACAAAAAAGCCAAACCCTCGTAGCTGGGTTACTTACCA‑CTGTTGCGTCATGACGCGGAAAAATCGCGGCGTAACTTCCACTATGACACCAACGTTGGggatggat                                                                                                                                                                                       >  2:349742/1‑251 (MQ=255)                                                               agCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCAAGTTGTCACGCCGAACAAAAAGGCCAACACCCCGTCGCTGGCTTACT‑AACATCTGTTGCGTCATGCGGCGGAAAAATAGCGGCGTAAATTCCTCTATGACCACAACGTTGGGGCTGGAtt                                                                                                                                                                                      >  1:50060/1‑250 (MQ=255)                                                                       aaTCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTGTCCACGTTGTCACGCCGAACAAAAAGGCCAACTCCTCGTCGATTGATTACT‑ACCTTCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAGTCCTCGATGACTCCAACGTTGGGGCTGGATTACCGGTTa                                                                                                                                                                              <  1:306139/250‑1 (MQ=255)                                                                              ggCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTGCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCAGtt                                                                                                                                                                                                                                                     <  1:168848/172‑1 (MQ=255)                                                                                                    ataaTATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCTCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCTGTTATTGAGAACCTGCAAAATCTGCTCAATGctg                                                                                                                                                <  1:116819/249‑3 (MQ=255)                                                                                                     gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAAAACCTCGTCGGTGGGTTACT‑ACCATCTGTTTCGTCATACGGAGGAAAAATAGCGGCGTAAAATCATCTATGGCACCAAAGTTGGGGCTGGATTACCGGTTATTGAGAACCTGACAAATCGGCTCAATGctgg                                                                                                                                               >  1:179705/1‑248 (MQ=255)                                                                                                     gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAACATGCCGACTTCTTTCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAAGCCACCACCTCGTCGGTGGATTCAT‑CACATCTGTTGCGGCATGCGGCGGACAAATCCCGGCGCAACTTACTCTTTAACACCAACGTTGGGGCGGGATTTCCGGTTATTTATACACTGCAAAAACTGCCCAATGctgg                                                                                                                                               >  2:37166/1‑248 (MQ=255)                                                                                                                gctgAACCCGGTCATTGTTGACCGCACGTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAAtt                                                                                                                                      <  1:72705/249‑1 (MQ=255)                                                                                                                  tgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACCAAAAGGCCAACACCTCGTCGATGGATTAAT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGAATtgatg                                                                                                                                  >  1:365732/1‑251 (MQ=255)                                                                                                                      cccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCCCGTTGTCACGCCGACCAAAAAGGCCAACACCTCGTCGATGGATTACT‑CCCATCAGTTGTGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTCTGACACCAACGTTGGGGCTGGGTTACCGGTTATTGAGACCCTGCAAAATCTGCTCCATGTAGGTGATGCATTGATGAAGt                                                                                                                              >  1:47531/1‑251 (MQ=255)                                                                                                                         ggTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTGTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGTATTGATGAAGTtct                                                                                                                           <  1:305939/251‑1 (MQ=255)                                                                                                                          ttCATTGTTTACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATACCGACTTCTTGCGCGAAGGGTTCCACGTTGTCACGCCAAACAAAAAGGCCAAAACCTCGTCGTTGTATTAAT‑ACCATCTGTTAGGTCATACTGCGGAAAAATCGCGGAGTATATTACTATTAGAAAACAACGTTGGGACAGGATTAACGGTGATTGAGAAACCGAACAATCTGCACAGTGCTGGTAGTGAATTTATGAAgagctc                                                                                                                          >  2:264943/2‑246 (MQ=255)                                                                                                                                        cACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttcag                                                                                                            <  1:154121/251‑3 (MQ=255)                                                                                                                                                  cagGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTc                                                                                                  >  1:193580/1‑251 (MQ=255)                                                                                                                                                                   atatGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGt                                                                                 >  1:62486/1‑251 (MQ=255)                                                                                                                                                                    aatGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATTGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt                                                                                <  2:62486/250‑1 (MQ=255)                                                                                                                                                                     atGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt                                                                                >  1:100147/1‑250 (MQ=255)                                                                                                                                                                     atGCCGAATTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATATGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCACGttt                                                                               >  2:161573/1‑250 (MQ=255)                                                                                                                                                                        ccGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGAc                                                                            <  1:37166/251‑1 (MQ=255)                                                                                                                                                                             ttcttGCGCGAAGGTTTCCACGTTGTCACGCCGAACAACAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTg                                                                                                                                                                   >  1:89547/5‑159 (MQ=255)                                                                                                                                                                             ttcttGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTg                                                                                                                                                                   <  2:89547/155‑1 (MQ=255)                                                                                                                                                                                      gAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAACATCGCGGCGTCATTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGACACTGCAACATCTGCTCAATGCTGGTGATGAATTGATGACGTTCTCCGTCATTCTTTCGGGTCCGCTTTCTTATATCTTCGGTACGTTAGACGAAGGCATGAtttt                                                              >  2:208696/1‑251 (MQ=255)                                                                                                                                                                                      gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt                                                              >  1:80426/1‑251 (MQ=255)                                                                                                                                                                                       aaGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTtc                                                             >  1:77929/1‑251 (MQ=255)                                                                                                                                                                                                actttGTCACGCCGACCAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATGAGTTGTGTTTTGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAGTGAGAACCTGCAAAATCGGCTCAATGCAGGTGATGAATTGATGAATTGCTCCGGCATTCGTTCTGGTGCGCTTTCGTATATCTTCGGGAAGTTAGGCGAAGGCATCAGTTGCTCCGAGGCg                                                    <  2:47531/248‑1 (MQ=255)                                                                                                                                                                                                               aCAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATATTCGGCAAGTTAGACGAAGGCATGAGTTTATCCGAGGCGACTACTCTGGCGCgg                                     >  1:215758/1‑251 (MQ=255)                                                                                                                                                                                                                             acCTCGTAGATGGATTACT‑ACATTCTGTAGCGTCATGCGGAGGACAAATCGCGGCGTAAATTCCTCTATGACACCCACGTTGGGGCTGGATTACCGGTTACTGAGAACCTGCACCATTTGCTCAATGCTGGTGATGCATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGATAATGGGTTATAc                       >  2:142373/1‑251 (MQ=255)                                                                                                                                                                                                                                tcgtcgATGGATTACT‑ACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCg                     >  1:137882/1‑250 (MQ=255)                                                                                                                                                                                                                                                   cATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatga  <  1:288394/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACT‑ACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGA  >  NC_000913/1863‑2354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑