Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,899	T→C	D503D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,899	0	T	C	91.2%	137.4 / 4.8	57	D503N (GAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (21/31);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

CAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTA  >  NC_000913/23659‑24137                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAgg                                                                                                                                                                                                                                          >  1:195622/1‑251 (MQ=255)                    cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGTCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                                                       >  2:94342/1‑251 (MQ=255)                    cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                                                       >  1:138596/1‑251 (MQ=255)                      gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCTGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTATCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTTCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                                                       <  1:227712/249‑1 (MQ=255)                      gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATg                                                                                                                                                                                                                        <  1:161682/248‑1 (MQ=255)                             cAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAag                                                                                                                                                                                                                  >  1:201653/1‑247 (MQ=255)                             cAGTCACTGAAAGAGAGTAAAGGCGTGCAGTGGATCCCGGACTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGTCTGGTGTATCTCCCGGCAGCTCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAATACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGAt                                                                                                                                                                                                              <  1:266843/251‑1 (MQ=255)                                          agaTGAAAGGCGTGCAGTGGTTCCCGGACTGGGGCCAGGCGCGTATCGAGGCGATGGTGGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGAGACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAGCGCTGTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCTATGCGAAATAGATCCTTGGT‑GACGaa                                                                                                                                                                                                <  2:182200/251‑1 (MQ=255)                                            aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGC‑GACGAAGc                                                                                                                                                                                              <  1:152069/251‑1 (MQ=255)                                                   gCGTGCAGTGTGTCCCGGACTGGGTCCAGGTGCGTAGTGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTTGCTCCCGTCAGCGCTCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTGGAAGTCAACGGCATCCAGGTGTGGTGGGATCTCGATTCGAAAGATATCCTCGGC‑GACGAAGCTGACCA‑g                                                                                                                                                                                      <  1:330121/251‑1 (MQ=255)                                                        cAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTatgt                                                                                                                                                                                 <  1:59856/251‑4 (MQ=255)                                                         aGTGGATCACGGACTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTAAGCGCACCTGTCGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAACCGCGTTGAAGTTCACGCCATCCAGGCGCGGTGGGATCTCGATGCGAAACACATCCCCGCc‑gacgagactgaccggtagc                                                                                                                                                                                   >  2:114788/1‑235 (MQ=255)                                                                 ccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGcc                                                                                                                                                                       <  1:221693/251‑1 (MQ=255)                                                                  cGGACTGGGGCCAGGGGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCg                                                                                                                                                                      <  1:278266/251‑1 (MQ=255)                                                                  cGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTAGATGCGAAAGAGATCCTCGGC‑GACGAAGTTGACCA‑GTATGTGAA‑AGTGCCg                                                                                                                                                                      <  1:137642/251‑1 (MQ=255)                                                                    gACTGGGGCCAGGCGCGTATCGAGTTGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGa                                                                                                                                                                    <  1:13802/251‑1 (MQ=255)                                                                    gACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGa                                                                                                                                                                    <  1:209828/251‑1 (MQ=255)                                                                                gcgcGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGatactctcgacgt                                                                                                                                                        <  1:288056/251‑13 (MQ=255)                                                                                    gTATCGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGatactctcgacgtatgg                                                                                                                                                    <  1:228158/251‑17 (MQ=255)                                                                                     tctCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAAGTTACCGGCATCCAGGCGTGGGGGGATCGCGTTGCGATAGAGATCCTCGGCAGA‑GAAGCTGACCA‑GTATGTGAA‑AGGGCCGGatactctcgaggtagggt                                                                                                                                                   >  2:282445/3‑234 (MQ=255)                                                                                          aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGAc                                                                                                                                             >  1:343441/1‑251 (MQ=255)                                                                                             cggtGGTTTCTAACCGTCCTGACTGGTGTCACTCCCTGCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGGTCACAAAGACGCGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAATAAGTTGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACTAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTcc                                                                                                                                          <  2:142012/248‑1 (MQ=255)                                                                                              gctggtttatatccGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGACAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGCAGTATGGTTTGAATCCg                                                                                                                                         >  2:251117/13‑251 (MQ=255)                                                                                                        aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTAcc                                                                                                                                 <  1:43918/249‑1 (MQ=255)                                                                                                                 gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                                                                                                                       <  1:97079/250‑1 (MQ=255)                                                                                                                   cTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCC‑GTATGTGAA‑AGTGCCGGATACTC‑TCGCAGTATGGGTTGACTCCGGATCTACCCACTCTTCtgttg                                                                                                                    >  2:66719/1‑251 (MQ=255)                                                                                                                                cGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTcc                                                                                                       <  1:130454/251‑1 (MQ=255)                                                                                                                                      cgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAtt                                                                                                 <  1:9669/251‑1 (MQ=255)                                                                                                                                          ccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc                                                                                             >  2:88762/1‑251 (MQ=255)                                                                                                                                            tGGGGCGTACCGCTGTCACTGTTCGTGCCCAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGC‑GACGAAGCTGA‑CACGTATGTGTA‑AGAGCCGGATA‑ATCTCGACGTATGGGTTGACTACGGATCTACCCACTCTTCTGTTGTTGCCTTTCGTCCGGAATTTGCCgg                                                                                           >  2:200705/1‑251 (MQ=255)                                                                                                                                             gtggcgtACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGGGTGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGGATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAATTTGCCg                                                                                            <  1:151906/244‑1 (MQ=255)                                                                                                                                               ggcgttccattttctcTGTACGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCATCGAACTGATGGAAGAATTGGCAAAACGAGTTGAAGTTAACGGCTTCCAGGTGTGGTGGGTTCTCGTTGCGAAATAGATCCGCGGA‑GACGAAGCTGACAA‑TTATGTGAA‑AGTGCCGGATGCTC‑TCGGCGTATGGTTTGACTTCGGATGTACTCACTCTTCTGTTTTTGTCGTGCGTCCGGAATTTGTCGGtta                                                                                        <  2:287993/236‑3 (MQ=255)                                                                                                                                                  gtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGAGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAACAGT‑CCGGATACAC‑ACCAAGTATGGTTTGACTCCGGATCTACCACCTCTTATGTTGTTGACGGGCGTACGGCATTTGCCGGTCACGc                                                                                     >  2:338675/1‑251 (MQ=255)                                                                                                                                                  gtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAAATGATGGCAGAAGTGGCAAAACGCGTTGCAGTTAACGGCATACAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑CGTGCCGGAGACTC‑TCGGCGAATGGTTTGACTCCGGATCTACCCACTCTTCTTTTGTTGACGTGCATCCGGAATTTGCCGGTctctc                                                                                     >  2:322366/1‑247 (MQ=255)                                                                                                                                                   tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                                                     >  1:369161/1‑250 (MQ=255)                                                                                                                                                      cGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                                                 >  1:60791/1‑251 (MQ=255)                                                                                                                                                            cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATg                                                                           >  1:189274/1‑251 (MQ=255)                                                                                                                                                                gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                                                       <  1:25955/251‑1 (MQ=255)                                                                                                                                                                   cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCC‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTgg                                                                    >  1:344671/1‑251 (MQ=255)                                                                                                                                                                     ttcacaAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGACGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTTGTGGGATATCGATGCGAAAGAGATCCTCGGC‑GACGATGCTGACCA‑TAATGTGAA‑AGTGACGGATACTC‑TCGCCGTATGGTTTGACTCCGGATATACCCACTCTGCTGCTGTTGACGTGCGTCCGGAGTTTGCAGGTAACGCAGCGGAccagtatccggt                                                                   >  2:265887/3‑239 (MQ=255)                                                                                                                                                                         caAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGGTGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGTCCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTGGACTCCGGATCTACCCACTCTTCTGTTGTTGACGGGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGtt                                                              <  1:208257/251‑1 (MQ=255)                                                                                                                                                                               cacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                                                        <  1:94342/251‑1 (MQ=255)                                                                                                                                                                                       agctgcatccgtgggacctcggagtGATGGAAGAATTGGCAAAATGCGCTGAAGGTAACGGCATCCAGGCTGGGTGTGAGCTCGCTGCGAAAGAGTTCCTCGTC‑GACGAAGCAGACCA‑GTATGTGAA‑AGTGCCGGAGACTC‑TCGACGTATTGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGAAGTGCGTCCGGAATTTGCCGTTCACGCAGCGGACATGTATCTGGAAGGTCCTGACCAGCACcgt                                                <  2:305355/227‑2 (MQ=255)                                                                                                                                                                                       agCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGAcccgcaccgt                                                >  1:221614/1‑243 (MQ=255)                                                                                                                                                                                        gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg                                               <  1:312788/251‑3 (MQ=255)                                                                                                                                                                                          tGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggt                                             >  1:170413/1‑247 (MQ=255)                                                                                                                                                                                                 gcgTACCATTGAAATGATGGAAGAAGTGGAAAAAAGAGTTGAAGTAGATGGTATCAAGGAGTGGTGGGATATAGATGAGAAAGAGATAATAGGA‑GAAGAAGATGATCA‑GTAAGTGAA‑AGTGCAGGAAAAAT‑TGGATGTATGGTTTGAATACGGATATAAAAAATATTATGTTGTTGACGTGCGTACGGAATTTGCAGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGAGGCTGGTTCa                                      <  1:251117/251‑1 (MQ=255)                                                                                                                                                                                                  cgaaacctcgAACAGATGGAAGAAGTGGGAAAACGCTGTGAAGTTAACGGCATCCAGGCGTGGCGGGATCTCGATGCGAAATAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGGATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGGCCGGAATTTGCTGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtttgttcga                                     <  2:13978/242‑13 (MQ=255)                                                                                                                                                                                                           gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCt                            <  1:99796/251‑1 (MQ=255)                                                                                                                                                                                                               tGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GAAGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATg                        >  1:229218/1‑251 (MQ=255)                                                                                                                                                                                                                gATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGTCATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGa                       <  1:16331/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAAGAAGTGGCAAAACGCGTTGAATTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGATGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtct                    <  1:242797/251‑1 (MQ=255)                                                                                                                                                                                                                       gaagTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTTATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTACGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGGAGGTTCTGACCAACAACGCGGCTGGTTCATGTCTTCCCTAATGATCTCCAc                <  1:156967/251‑1 (MQ=255)                                                                                                                                                                                                                                  aCGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa       >  1:187875/1‑249 (MQ=255)                                                                                                                                                                                                                                   cgcgTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaaagg    <  1:111146/251‑4 (MQ=255)                                                                                                                                                                                                                                    gcgTTGAAGTTAACGGAATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGC‑CACGAAGCTGACCA‑GTATGTAAA‑AGTGCCGGATACTC‑TAGAAGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGCAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGTTCTCCACCGCGCTGaaaggc   >  1:377387/1‑247 (MQ=255)                                                                                                                                                                                                                                     cgTTGAAGTTAACGGCATCCAGGTGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACTAAGCTGACTA‑GTATGTGAA‑AGTGCCTGATACTC‑TCGACGTATGGTTTGGCTTCGGATCTACCCTCTCTTCTGGTGTTGACGTGCGTCCGGAATTTGCTGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAGCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTGCACCGCGATGaaaggca  <  1:18002/251‑6 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   CAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTA  >  NC_000913/23659‑24137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑