Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,860	T→C	L490L (CTT→CTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,860	0	T	C	94.7%	151.8 / ‑0.2	57	L490L (CTT→CTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (23/31);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01

CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCA  >  NC_000913/23615‑24101                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAAc                                                                                                                                                                                                                                                   <  1:152546/250‑1 (MQ=255)  acaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCACGTACCCTCGAACTg                                                                                                                                                                                                                                                 >  1:334948/1‑251 (MQ=255)         gCCGATCATCTGCCGCGAGACGCCGCCGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGAGCAAAGGCGTGCAGGGGATCCCGTA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCTCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaag                                                                                                                                                                                                                                          <  2:128006/251‑1 (MQ=255)           cGATCATCTTCAGCGCGACGCCGAAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGAGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGGCTGGTGTATCTCCCGTCAGCGCACCTGGGGGGGACCGCTGTCACTGTTCGTGCACAACGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGCTGgaagc                                                                                                                                                                                                                                         >  2:156967/1‑249 (MQ=255)              tcatcTACCGAGCGACGCCGCAGTGGTTCGGCAGCATGAATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGTGATCAAGGGCATGCAGTGGATCCCGGA‑CTGGGGACAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTCTCTCCCGTCAGCGCAACTGGGGCGTCCGGATGTCACTGTTCGTGACCAAAGACCCGGAAACTCTGCATCCGCAGACCCTCGTACTGATGGAAGAAGTg                                                                                                                                                                                                                                     >  2:354819/1‑251 (MQ=255)                     ccGCGCGATGCCGCAGTTGTTCGTCAGCATGGAGCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATTGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCCCGAACTGATGGAAGAAGTGGCAAAAc                                                                                                                                                                                                                              <  1:262870/251‑1 (MQ=255)                           gACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                                                                                        >  1:346960/1‑251 (MQ=255)                               cagcAGTGGTTCGTCAGCATGGACCAGAAAGGTCTGCGTGCGCAGCCTCTGAAAGAGATCAAAGGCGTGCAGGTGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAGGTAAGAATTGGCAAATCGCGTTGAAGt                                                                                                                                                                                                                    <  2:169474/249‑1 (MQ=255)                                         gttgtatactGGATCAGAAGGGTCGGCGTGCGCATTCCATGTACGATACCAAATGCGTGCAGTGGTTCCCGGATCCGGGTCTAG‑CGCTTAGCGAGACGATGGTTGCTTCTCGTCCTGACTGGTGTTGCTCCCGTCAGCGCACCTGAGGCGGACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCGCACTGATGGAAGAAGTGGCTAAACGCGTTGAAGttaactgcct                                                                                                                                                                                                           <  2:318516/241‑10 (MQ=255)                                             cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAgg                                                                                                                                                                                                      >  1:195622/1‑251 (MQ=255)                                                                cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGTCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                   >  2:94342/1‑251 (MQ=255)                                                                cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                   >  1:138596/1‑251 (MQ=255)                                                                  gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCTGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTATCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTTCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                                                                                   <  1:227712/249‑1 (MQ=255)                                                                  gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATg                                                                                                                                                                                    <  1:161682/248‑1 (MQ=255)                                                                         cAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAag                                                                                                                                                                              >  1:201653/1‑247 (MQ=255)                                                                         cAGTCACTGAAAGAGAGTAAAGGCGTGCAGTGGATCCCGGA‑CTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGTCTGGTGTATCTCCCGGCAGCTCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAATACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGAt                                                                                                                                                                          <  1:266843/251‑1 (MQ=255)                                                                                      agaTGAAAGGCGTGCAGTGGTTCCCGGA‑CTGGGGCCAGGCGCGTATCGAGGCGATGGTGGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGAGACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAGCGCTGTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCTATGCGAAATAGATCCTTGGT‑GACGaa                                                                                                                                                            <  2:182200/251‑1 (MQ=255)                                                                                        aTCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGC‑GACGAAGc                                                                                                                                                          <  1:152069/251‑1 (MQ=255)                                                                                               gCGTGCAGTGTGTCCCGGA‑CTGGGTCCAGGTGCGTAGTGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTTGCTCCCGTCAGCGCTCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTGGAAGTCAACGGCATCCAGGTGTGGTGGGATCTCGATTCGAAAGATATCCTCGGC‑GACGAAGCTGACCA‑g                                                                                                                                                  <  1:330121/251‑1 (MQ=255)                                                                                                    cAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTatgt                                                                                                                                             <  1:59856/251‑4 (MQ=255)                                                                                                     aGTGGATCACGGA‑CTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTAAGCGCACCTGTCGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAACCGCGTTGAAGTTCACGCCATCCAGGCGCGGTGGGATCTCGATGCGAAACACATCCCCGCc‑gacgagactgaccggtagc                                                                                                                                               >  2:114788/1‑235 (MQ=255)                                                                                                             ccGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGcc                                                                                                                                   <  1:221693/251‑1 (MQ=255)                                                                                                              cGGA‑CTGGGGCCAGGGGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCg                                                                                                                                  <  1:278266/251‑1 (MQ=255)                                                                                                              cGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTAGATGCGAAAGAGATCCTCGGC‑GACGAAGTTGACCA‑GTATGTGAA‑AGTGCCg                                                                                                                                  <  1:137642/251‑1 (MQ=255)                                                                                                                gA‑CTGGGGCCAGGCGCGTATCGAGTTGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGa                                                                                                                                <  1:13802/251‑1 (MQ=255)                                                                                                                gA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGa                                                                                                                                <  1:209828/251‑1 (MQ=255)                                                                                                                            ggCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaa                                                                                                                                                                                                                                           <  1:338702/136‑1 (MQ=255)                                                                                                                            ggCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaa                                                                                                                                                                                                                                           >  2:338702/1‑136 (MQ=255)                                                                                                                             gcgcGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGatactctcgacgt                                                                                                                    <  1:288056/251‑13 (MQ=255)                                                                                                                                 gTATCGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGatactctcgacgtatgg                                                                                                                <  1:228158/251‑17 (MQ=255)                                                                                                                                  tctCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAAGTTACCGGCATCCAGGCGTGGGGGGATCGCGTTGCGATAGAGATCCTCGGCAGA‑GAAGCTGACCA‑GTATGTGAA‑AGGGCCGGatactctcgaggtagggt                                                                                                               >  2:282445/3‑234 (MQ=255)                                                                                                                                       aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGAc                                                                                                         >  1:343441/1‑251 (MQ=255)                                                                                                                                          cggtGGTTTCTAACCGTCCTGACTGGTGTCACTCCCTGCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGGTCACAAAGACGCGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAATAAGTTGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACTAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTcc                                                                                                      <  2:142012/248‑1 (MQ=255)                                                                                                                                           gctggtttatatccGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGACAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGCAGTATGGTTTGAATCCg                                                                                                     >  2:251117/13‑251 (MQ=255)                                                                                                                                                     aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTAcc                                                                                             <  1:43918/249‑1 (MQ=255)                                                                                                                                                              gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                                                                                   <  1:97079/250‑1 (MQ=255)                                                                                                                                                                cTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCC‑GTATGTGAA‑AGTGCCGGATACTC‑TCGCAGTATGGGTTGACTCCGGATCTACCCACTCTTCtgttg                                                                                >  2:66719/1‑251 (MQ=255)                                                                                                                                                                             cGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTcc                                                                   <  1:130454/251‑1 (MQ=255)                                                                                                                                                                                   cgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAtt                                                             <  1:9669/251‑1 (MQ=255)                                                                                                                                                                                       ccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc                                                         >  2:88762/1‑251 (MQ=255)                                                                                                                                                                                         tGGGGCGTACCGCTGTCACTGTTCGTGCCCAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGC‑GACGAAGCTGA‑CACGTATGTGTA‑AGAGCCGGATA‑ATCTCGACGTATGGGTTGACTACGGATCTACCCACTCTTCTGTTGTTGCCTTTCGTCCGGAATTTGCCgg                                                       >  2:200705/1‑251 (MQ=255)                                                                                                                                                                                          gtggcgtACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGGGTGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGGATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAATTTGCCg                                                        <  1:151906/244‑1 (MQ=255)                                                                                                                                                                                            ggcgttccattttctcTGTACGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCATCGAACTGATGGAAGAATTGGCAAAACGAGTTGAAGTTAACGGCTTCCAGGTGTGGTGGGTTCTCGTTGCGAAATAGATCCGCGGA‑GACGAAGCTGACAA‑TTATGTGAA‑AGTGCCGGATGCTC‑TCGGCGTATGGTTTGACTTCGGATGTACTCACTCTTCTGTTTTTGTCGTGCGTCCGGAATTTGTCGGtta                                                    <  2:287993/236‑3 (MQ=255)                                                                                                                                                                                               gtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGAGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAACAGT‑CCGGATACAC‑ACCAAGTATGGTTTGACTCCGGATCTACCACCTCTTATGTTGTTGACGGGCGTACGGCATTTGCCGGTCACGc                                                 >  2:338675/1‑251 (MQ=255)                                                                                                                                                                                               gtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAAATGATGGCAGAAGTGGCAAAACGCGTTGCAGTTAACGGCATACAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑CGTGCCGGAGACTC‑TCGGCGAATGGTTTGACTCCGGATCTACCCACTCTTCTTTTGTTGACGTGCATCCGGAATTTGCCGGTctctc                                                 >  2:322366/1‑247 (MQ=255)                                                                                                                                                                                                tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                 >  1:369161/1‑250 (MQ=255)                                                                                                                                                                                                   cGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                             >  1:60791/1‑251 (MQ=255)                                                                                                                                                                                                         cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATg                                       >  1:189274/1‑251 (MQ=255)                                                                                                                                                                                                             gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                   <  1:25955/251‑1 (MQ=255)                                                                                                                                                                                                                cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCC‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTgg                                >  1:344671/1‑251 (MQ=255)                                                                                                                                                                                                                  ttcacaAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGACGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTTGTGGGATATCGATGCGAAAGAGATCCTCGGC‑GACGATGCTGACCA‑TAATGTGAA‑AGTGACGGATACTC‑TCGCCGTATGGTTTGACTCCGGATATACCCACTCTGCTGCTGTTGACGTGCGTCCGGAGTTTGCAGGTAACGCAGCGGAccagtatccggt                               >  2:265887/3‑239 (MQ=255)                                                                                                                                                                                                                      caAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGGTGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGTCCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTGGACTCCGGATCTACCCACTCTTCTGTTGTTGACGGGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTACCTGGAAGGtt                          <  1:208257/251‑1 (MQ=255)                                                                                                                                                                                                                            cacGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGAcc                    <  1:94342/251‑1 (MQ=255)                                                                                                                                                                                                                                    agCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGAcccgcaccgt            >  1:221614/1‑243 (MQ=255)                                                                                                                                                                                                                                     gCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtg           <  1:312788/251‑3 (MQ=255)                                                                                                                                                                                                                                       tGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAA‑AGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggt         >  1:170413/1‑247 (MQ=255)                                                                                                                                                                                                                                              gcgTACCATTGAAATGATGGAAGAAGTGGAAAAAAGAGTTGAAGTAGATGGTATCAAGGAGTGGTGGGATATAGATGAGAAAGAGATAATAGGA‑GAAGAAGATGATCA‑GTAAGTGAA‑AGTGCAGGAAAAAT‑TGGATGTATGGTTTGAATACGGATATAAAAAATATTATGTTGTTGACGTGCGTACGGAATTTGCAGGTAACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGAGGCTGGTTCa  <  1:251117/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAA‑AGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCA  >  NC_000913/23615‑24101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑