Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,803	T→C	G471G (GGT→GGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,803	0	T	C	96.4%	142.6 / ‑2.6	57	G471G (GGT→GGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (23/30);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

AAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGG  >  NC_000913/23558‑24048                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         aaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGAATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTAcc                                                                                                                                                                                                                                                     >  1:15523/1‑251 (MQ=255) aaaGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCGGGCGTCACAAAACGACGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGGAGATGGTTGATACCCGTCCTGACTGGGGTATCTCCCGTCAGCGCACCTGGTGCGTAcc                                                                                                                                                                                                                                                     >  2:99796/1‑251 (MQ=255)       gcgcTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCAAGGCGCGTATCGAGTCGATGGTTGCTAACCACCCTTCCTGGTGTTTCTCCCGTCAGCGCACCTGGGGCGTACCgtcggc                                                                                                                                                                                                                                               >  2:147757/1‑246 (MQ=255)        cgcTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCa                                                                                                                                                                                                                                              <  1:270170/251‑1 (MQ=255)          ctgctgCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGCCGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGAGTATCGAGACGATGGTTGATAACCGTCCTGCCTGGAGTATCTCCCGTCAGCGCACCTGGGGCGTACCgcttgcact                                                                                                                                                                                                                                            >  2:25955/1‑243 (MQ=255)            gctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTTCTGGCGTCACAAAACACCGATCATCTTCCGCGCGAAGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCCCTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGATGTATCTCCCGTCAGCGCACCTGGGGCGTACCTATGtaacggt                                                                                                                                                                                                                                          >  1:51355/1‑245 (MQ=255)             ctgcACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAACCAGCCGATCATCTTCCGCGCGACGCCGCAGAGGTTCGTCAGCATGGATCAGACAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAGGGCGTGCAGTGGGTCCCGGA‑CGGGGGCCAGGCGCGTATCGAGTCGATGGATGCTAACCGTCCTGGATGGTGGATCACCCGTCAGCGCCCCCAGGGGGTACCGATGTCACTGtt                                                                                                                                                                                                                                         >  2:130454/1‑251 (MQ=255)                  cGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGc                                                                                                                                                                                                                                    >  1:98015/1‑251 (MQ=255)                      gataaaaTGCAGCACAGCTATCCGAGCAGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGATCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGAGACCCGTCCTGAATGGAGGATCTACCGTCATCGCACCTGAGGCGACCCGATGTCACTGTTccagccca                                                                                                                                                                                                                                 >  2:111146/4‑243 (MQ=255)                              gcagcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTATCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACg                                                                                                                                                                                                                         <  1:367010/250‑1 (MQ=255)                               cagcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGCGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGTCCAGGCGCGTATCGAGTCGATGGATGCTAACCGTCCTGACTGGTGCATCTCCCATCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGTGCACAAAGACACgg                                                                                                                                                                                                                        >  2:310494/1‑250 (MQ=255)                                agcaCAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGaa                                                                                                                                                                                                                      <  1:204492/251‑1 (MQ=255)                                  cacaGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGa                                                                                                                                                                                                                       >  1:13978/1‑248 (MQ=255)                                      gCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTg                                                                                                                                                                                                                >  1:305355/1‑251 (MQ=255)                                         aTCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCa                                                                                                                                                                                                              <  1:25393/250‑1 (MQ=255)                                                          cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTc                                                                                                                                                                                                                                        <  1:146701/207‑1 (MQ=255)                                                          cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAAc                                                                                                                                                                                             <  1:152546/250‑1 (MQ=255)                                                           acaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCACGTACCCTCGAACTg                                                                                                                                                                                           >  1:334948/1‑251 (MQ=255)                                                                  gCCGATCATCTGCCGCGAGACGCCGCCGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGAGCAAAGGCGTGCAGGGGATCCCGTA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGGCAGCGCTCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaag                                                                                                                                                                                    <  2:128006/251‑1 (MQ=255)                                                                    cGATCATCTTCAGCGCGACGCCGAAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGAGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGGCTGGTGTATCTCCCGTCAGCGCACCTGGGGGGGACCGCTGTCACTGTTCGTGCACAACGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGCTGgaagc                                                                                                                                                                                   >  2:156967/1‑249 (MQ=255)                                                                       tcatcTACCGAGCGACGCCGCAGTGGTTCGGCAGCATGAATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGTGATCAAGGGCATGCAGTGGATCCCGGA‑CTGGGGACAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTCTCTCCCGTCAGCGCAACTGGGGCGTCCGGATGTCACTGTTCGTGACCAAAGACCCGGAAACTCTGCATCCGCAGACCCTCGTACTGATGGAAGAAGTg                                                                                                                                                                               >  2:354819/1‑251 (MQ=255)                                                                              ccGCGCGATGCCGCAGTTGTTCGTCAGCATGGAGCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATTGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCCCGAACTGATGGAAGAAGTGGCAAAAc                                                                                                                                                                        <  1:262870/251‑1 (MQ=255)                                                                                    gACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                                  >  1:346960/1‑251 (MQ=255)                                                                                        cagcAGTGGTTCGTCAGCATGGACCAGAAAGGTCTGCGTGCGCAGCCTCTGAAAGAGATCAAAGGCGTGCAGGTGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAGGTAAGAATTGGCAAATCGCGTTGAAGt                                                                                                                                                              <  2:169474/249‑1 (MQ=255)                                                                                                  gttgtatactGGATCAGAAGGGTCGGCGTGCGCATTCCATGTACGATACCAAATGCGTGCAGTGGTTCCCGGATCCGGGTCTAG‑CGCTTAGCGAGACGATGGTTGCTTCTCGTCCTGACTGGTGTTGCTCCCGTCAGCGCACCTGAGGCGGACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCGCACTGATGGAAGAAGTGGCTAAACGCGTTGAAGttaactgcct                                                                                                                                                     <  2:318516/241‑10 (MQ=255)                                                                                                      cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAgg                                                                                                                                                >  1:195622/1‑251 (MQ=255)                                                                                                                         cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGTCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                             >  2:94342/1‑251 (MQ=255)                                                                                                                         cTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                             >  1:138596/1‑251 (MQ=255)                                                                                                                           gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCTGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTATCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTTCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGc                                                                                                                             <  1:227712/249‑1 (MQ=255)                                                                                                                           gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATg                                                                                                                              <  1:161682/248‑1 (MQ=255)                                                                                                                                  cAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAag                                                                                                                        >  1:201653/1‑247 (MQ=255)                                                                                                                                  cAGTCACTGAAAGAGAGTAAAGGCGTGCAGTGGATCCCGGA‑CTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGTCTGGTGTATCTCCCGGCAGCTCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAATACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGAt                                                                                                                    <  1:266843/251‑1 (MQ=255)                                                                                                                                               agaTGAAAGGCGTGCAGTGGTTCCCGGA‑CTGGGGCCAGGCGCGTATCGAGGCGATGGTGGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGAGACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAGCGCTGTGAAGTTAACGGCAGCCAGGCGTGGTGGGATCTCTATGCGAAATAGATCCTTGGT‑GACGaa                                                                                                      <  2:182200/251‑1 (MQ=255)                                                                                                                                                 aTCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGC‑GACGAAGc                                                                                                    <  1:152069/251‑1 (MQ=255)                                                                                                                                                        gCGTGCAGTGTGTCCCGGA‑CTGGGTCCAGGTGCGTAGTGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTTGCTCCCGTCAGCGCTCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTGGAAGTCAACGGCATCCAGGTGTGGTGGGATCTCGATTCGAAAGATATCCTCGGC‑GACGAAGCTGACCA‑g                                                                                            <  1:330121/251‑1 (MQ=255)                                                                                                                                                             cAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTatgt                                                                                       <  1:59856/251‑4 (MQ=255)                                                                                                                                                              aGTGGATCACGGA‑CTGGGTCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTAAGCGCACCTGTCGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAACCGCGTTGAAGTTCACGCCATCCAGGCGCGGTGGGATCTCGATGCGAAACACATCCCCGCc‑gacgagactgaccggtagc                                                                                         >  2:114788/1‑235 (MQ=255)                                                                                                                                                                      ccGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGcc                                                                              <  1:221693/251‑1 (MQ=255)                                                                                                                                                                       cGGA‑CTGGGGCCAGGGGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGGACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCg                                                                             <  1:278266/251‑1 (MQ=255)                                                                                                                                                                       cGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTAGATGCGAAAGAGATCCTCGGC‑GACGAAGTTGACCA‑GTATGTGAAAGTGCCg                                                                             <  1:137642/251‑1 (MQ=255)                                                                                                                                                                         gA‑CTGGGGCCAGGCGCGTATCGAGTTGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGa                                                                           <  1:13802/251‑1 (MQ=255)                                                                                                                                                                         gA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGa                                                                           <  1:209828/251‑1 (MQ=255)                                                                                                                                                                                     ggCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaa                                                                                                                                                                                     <  1:338702/136‑1 (MQ=255)                                                                                                                                                                                     ggCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGgaa                                                                                                                                                                                     >  2:338702/1‑136 (MQ=255)                                                                                                                                                                                      gcgcGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgacgt                                                               <  1:288056/251‑13 (MQ=255)                                                                                                                                                                                          gTATCGTGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgacgtatgg                                                           <  1:228158/251‑17 (MQ=255)                                                                                                                                                                                           tctCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAAGTTACCGGCATCCAGGCGTGGGGGGATCGCGTTGCGATAGAGATCCTCGGCAGA‑GAAGCTGACCA‑GTATGTGAAAGGGCCGGatactctcgaggtagggt                                                          >  2:282445/3‑234 (MQ=255)                                                                                                                                                                                                aGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGAc                                                    >  1:343441/1‑251 (MQ=255)                                                                                                                                                                                                   cggtGGTTTCTAACCGTCCTGACTGGTGTCACTCCCTGCAGCGCACCTGGGGCGTACCGATGTCACTGGTCGGTCACAAAGACGCGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAATAAGTTGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACTAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTcc                                                 <  2:142012/248‑1 (MQ=255)                                                                                                                                                                                                    gctggtttatatccGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGACAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACAT‑TGGCAGTATGGTTTGAATCCg                                                >  2:251117/13‑251 (MQ=255)                                                                                                                                                                                                              aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTAcc                                        <  1:43918/249‑1 (MQ=255)                                                                                                                                                                                                                       gACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCtg                              <  1:97079/250‑1 (MQ=255)                                                                                                                                                                                                                         cTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCC‑GTATGTGAAAGTGCCGGATACTC‑TCGCAGTATGGGTTGACTCCGGATCTACCCACTCTTCtgttg                           >  2:66719/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTcc              <  1:130454/251‑1 (MQ=255)                                                                                                                                                                                                                                            cgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAtt        <  1:9669/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTC‑TCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc    >  2:88762/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tGGGGCGTACCGCTGTCACTGTTCGTGCCCAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGC‑GACGAAGCTGA‑CACGTATGTGTAAGAGCCGGATA‑ATCTCGACGTATGGGTTGACTACGGATCTACCCACTCTTCTGTTGTTGCCTTTCGTCCGGAATTTGCCgg  >  2:200705/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         AAAGGCGCGCTGCTGCACGTTGAGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGA‑CTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGC‑GACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACAT‑TGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGG  >  NC_000913/23558‑24048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑