Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,297	C→T	L303L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,297	0	C	T	91.4%	84.0 / ‑1.8	39	L303L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (15/17);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAC  >  NC_000913/23052‑23533                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               cACTGAAAGCAAGATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAACACGGTAAAAGGTG‑CGGAG‑GTTGAGgtgctg                                                                                                                                                                                                                                           >  1:259725/1‑251 (MQ=255)    tGAAAGCAAAATTTCCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTgcgc                                                                                                                                                                                                                                        >  1:311499/1‑251 (MQ=255)     gAAAGCAAAATTTGCCGGAAGCAACGGTAACGGCTCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACGATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGAC‑CTATTACACCAGTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGGTGCTgcg                                                                                                                                                                                                                                         <  2:378096/249‑1 (MQ=255)          caaaattttccgatatcaactgtAACGGCCCAATCTCGCTGGTGATCTGGACCTCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGAGTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGG‑TTTAca                                                                                                                                                                                                                                 <  2:157882/229‑2 (MQ=255)                  tccGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGt                                                                                                                                                                                                                          <  2:168669/249‑1 (MQ=255)                     gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTAt                                                                                                                                                                                                                      <  1:103580/251‑1 (MQ=255)                           aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑AGATTAAACCATTCTCCACACGGAAAAAGGTGACGG‑G‑CTTGAGTTGCTTCGC‑TTTACCCATCCGTTTATGGGCtt                                                                                                                                                                                                                >  2:273366/1‑251 (MQ=255)                            aCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTc                                                                                                                                                                                                               <  1:368873/251‑1 (MQ=255)                             cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTACAAGGGG‑CGGAG‑ATTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGACTTCg                                                                                                                                                                                                              >  1:42938/1‑251 (MQ=255)                                         aaTCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCCTTCTCAGAACGGTAAAAGGAG‑CGGGG‑TTTGAGATGCCGCGC‑AGTACCCATCCGTTTATGGGCCTCGACGTACCGGcc                                                                                                                                                                                                   >  2:145304/1‑249 (MQ=255)                                            ctcGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGATAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGGAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATcc                                                                                                                                                                                               >  1:84614/1‑251 (MQ=255)                                               gCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGAGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGTCGCAACGCG‑TTACAACATTCTCAGCACGGAAAAAGG‑G‑ACGAGATTTGTGTTGGTGGGC‑TTTACCCATCCGTTTATGAGCtttggcggtctggaactacacg                                                                                                                                                                                            >  2:303397/1‑231 (MQ=255)                                                                    gCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATg                                                                                                                                                                       <  1:93284/251‑1 (MQ=255)                                                                                    gcgaaCCGCGCAATCTCTATTGCATCTGATTTCGACTATGCGCTTGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCg                                                                                                                                                        <  1:148750/247‑1 (MQ=255)                                                                                    gccAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGt                                                                                                                                                       >  1:189423/1‑251 (MQ=255)                                                                                           gcgcAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACAcc                                                                                                                                                <  1:160371/251‑1 (MQ=255)                                                                                              cactctcTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACc‑gcg                                                                                                                                            <  1:235491/248‑1 (MQ=255)                                                                                                 tctcTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGAGTGAC‑AGATAACACCAATCTCCGCAAGGGAAAAGGGG‑TGGAG‑CGTGAGTTGCT‑CGCCTTCACCCATCCGTTTAAGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGATACCAGAGATGCCTGTACCgatcccattcccacccgcacc                                                                                                                                          >  2:310830/1‑231 (MQ=255)                                                                                                         gCACCTGACTTCGAGTATGAGTTGGTGCAGATCGACGGTCAGGTCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACTCCATTCCCAGCCCGGTAAAAGGTG‑CGGAG‑CTTGAGTGGCTGCGC‑TTTGCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTGCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCAACg                                                                                                                                  <  2:283002/250‑1 (MQ=255)                                                                                                           acctgATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCACC‑GCGCCTGGCCACGGcc                                                                                                                               >  1:128006/5‑251 (MQ=255)                                                                                                                        tATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGGTCACACC‑GCGCCTGGCCACGGCCCGGACGACTAtgt                                                                                                                  >  1:313630/1‑251 (MQ=255)                                                                                                                                ggTGAAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGCTCTGGTTGAAAGCGTAATGCAGCATATCGGCGTGAC‑CGACTACCACATTCTCAGCACAGCAAAAGGGG‑CGGCG‑CTTGAGATTCTGAGC‑TTTACCCCTCCGTTTATAGGCTTCGACGTTCCGGCAGTCCGCGGCGATACCGTTACCCTGTATTCCTGTGGCGGTGCTGTTC‑CACCCTCGCCTGGCCAccgacaggacgctcatggtctaggac                                                                                                          >  2:116168/1‑226 (MQ=255)                                                                                                                                             ggTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCt                                                                                             <  1:362690/251‑1 (MQ=255)                                                                                                                                             ggTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGccg                                                                                             >  1:306341/1‑250 (MQ=255)                                                                                                                                              gTCAGGCCTTGGTTCTGGCGAAAGATCTGGTTGAAAGCGGAATGACGCGTATCGGCGTGAC‑CTATTACACCAGACTCAGCACGGTAAAATGTG‑CGGAG‑CTTGAGGTGCGTCGC‑GTTGCCCATCCGTGTATGGGCTTCGACGGTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTAGGTGATCGGTCAGAAATACTGCCTg                                                                                            <  2:283388/251‑1 (MQ=255)                                                                                                                                                     cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CATGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGACTGGAAACCg                                                                                     >  1:79210/1‑251 (MQ=255)                                                                                                                                                                          ggTTTAAAGCGTAATGCAGCGTTTCGGAGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                    <  2:6508/247‑1 (MQ=255)                                                                                                                                                                                    gTAATGCTGCGTATCGGCGGGAC‑CTATTACACCTATCTCAGCTCGGTAAATGGGG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAGCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTATCCCGGTTGGCCCGGACGGCACTTATCt                                                      <  2:230837/251‑1 (MQ=255)                                                                                                                                                                                           aGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGc                                               >  1:255810/1‑251 (MQ=255)                                                                                                                                                                                             cGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCAc                                             <  1:273366/251‑1 (MQ=255)                                                                                                                                                                                                    gCGTGAC‑CGATTACAGCATGCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGTCAATCCTCGGCGATCACGTTACCCTGGAGGCCGGTACCGGGGCCGTTCGCACC‑GCGCCTGGCCACGGCCCGGACTACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                                      <  1:352618/251‑1 (MQ=255)                                                                                                                                                                                                        gAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGc                                  >  1:229073/1‑251 (MQ=255)                                                                                                                                                                                                             gATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                                                  >  1:291884/1‑104 (MQ=255)                                                                                                                                                                                                             gATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                                                  <  2:291884/104‑1 (MQ=255)                                                                                                                                                                                                                        tctcAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc                    <  1:116168/250‑1 (MQ=255)                                                                                                                                                                                                                         ctcagCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATAGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGCACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGACAGAAATAAGGCATAGAAACCGCTAAACCGGTTGGACCGGCCGGAACTTATATGACGGGCACTTATACGCCGCTGGATGGCgggcacg                   >  2:204492/5‑244 (MQ=255)                                                                                                                                                                                                                                   tAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCg        >  1:92049/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGGTCCGGCAATCCTCGGCGCTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCCCGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACg    <  2:84614/251‑1 (MQ=255)                                                                                                                                                                                                                                         gTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAc  >  1:84239/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAc  <  1:193650/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               CACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAC  >  NC_000913/23052‑23533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑