Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,270	G→A	G294S (GGC→AGC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,270	0	G	A	95.0%	106.2 / ‑4.0	42	G294S (GGC→AGC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (16/22);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23030‑23515                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          tttcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaa                                                                                                                                                                                                                                                  <  1:211986/247‑1 (MQ=255)     aGGCGGTCGATCAGGGTGCACTGAAAGCAAAATTTGCCGTACGCAACGTTAACCGCCCAAGCTCGCTGGTGCTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTGTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaaaatgt                                                                                                                                                                                                                                             <  1:346754/251‑4 (MQ=255)          gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑Cggg                                                                                                                                                                                                                                       >  1:6508/1‑250 (MQ=255)            cGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑c                                                                                                                                                                                                                                    <  1:126546/251‑1 (MQ=255)              aTCAGGATGCACTGAAAGCAAAATTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAATATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑Ctt                                                                                                                                                                                                                                  <  1:170099/251‑1 (MQ=255)                 aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTg                                                                                                                                                                                                                                 >  1:359574/1‑249 (MQ=255)                  ggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGAAATGCAGCGGATCGGCGTGAC‑CGATTACACCATTTTCAGCACGGTAACAGGTG‑CGGAG‑CTTGAgt                                                                                                                                                                                                                              >  2:62424/1‑250 (MQ=255)                       cACTGAAAGCAAGATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAACACGGTAAAAGGTG‑CGGAG‑GTTGAGgtgctg                                                                                                                                                                                                                         >  1:259725/1‑251 (MQ=255)                          tGAAAGCAAAATTTCCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTgcgc                                                                                                                                                                                                                      >  1:311499/1‑251 (MQ=255)                           gAAAGCAAAATTTGCCGGAAGCAACGGTAACGGCTCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACGATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGAC‑CTATTACACCAGTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGGTGCTgcg                                                                                                                                                                                                                       <  2:378096/249‑1 (MQ=255)                                caaaattttccgatatcaactgtAACGGCCCAATCTCGCTGGTGATCTGGACCTCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGAGTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGG‑TTTAca                                                                                                                                                                                                               <  2:157882/229‑2 (MQ=255)                                        tccGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGt                                                                                                                                                                                                        <  2:168669/249‑1 (MQ=255)                                           gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTAt                                                                                                                                                                                                    <  1:103580/251‑1 (MQ=255)                                                 aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑AGATTAAACCATTCTCCACACGGAAAAAGGTGACGG‑G‑CTTGAGTTGCTTCGC‑TTTACCCATCCGTTTATGGGCtt                                                                                                                                                                                              >  2:273366/1‑251 (MQ=255)                                                  aCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTc                                                                                                                                                                                             <  1:368873/251‑1 (MQ=255)                                                   cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTACAAGGGG‑CGGAG‑ATTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGACTTCg                                                                                                                                                                                            >  1:42938/1‑251 (MQ=255)                                                               aaTCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCCTTCTCAGAACGGTAAAAGGAG‑CGGGG‑TTTGAGATGCCGCGC‑AGTACCCATCCGTTTATGGGCCTCGACGTACCGGcc                                                                                                                                                                                 >  2:145304/1‑249 (MQ=255)                                                                  ctcGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGATAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGGAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATcc                                                                                                                                                                             >  1:84614/1‑251 (MQ=255)                                                                     gCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGAGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGTCGCAACGCG‑TTACAACATTCTCAGCACGGAAAAAGG‑G‑ACGAGATTTGTGTTGGTGGGC‑TTTACCCATCCGTTTATGAGCtttggcggtctggaactacacg                                                                                                                                                                          >  2:303397/1‑231 (MQ=255)                                                                                          gCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATg                                                                                                                                                     <  1:93284/251‑1 (MQ=255)                                                                                                          gcgaaCCGCGCAATCTCTATTGCATCTGATTTCGACTATGCGCTTGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCg                                                                                                                                      <  1:148750/247‑1 (MQ=255)                                                                                                          gccAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGt                                                                                                                                     >  1:189423/1‑251 (MQ=255)                                                                                                                 gcgcAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACAcc                                                                                                                              <  1:160371/251‑1 (MQ=255)                                                                                                                    cactctcTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACc‑gcg                                                                                                                          <  1:235491/248‑1 (MQ=255)                                                                                                                       tctcTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGAGTGAC‑AGATAACACCAATCTCCGCAAGGGAAAAGGGG‑TGGAG‑CGTGAGTTGCT‑CGCCTTCACCCATCCGTTTAAGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGATACCAGAGATGCCTGTACCgatcccattcccacccgcacc                                                                                                                        >  2:310830/1‑231 (MQ=255)                                                                                                                               gCACCTGACTTCGAGTATGAGTTGGTGCAGATCGACGGTCAGGTCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACTCCATTCCCAGCCCGGTAAAAGGTG‑CGGAG‑CTTGAGTGGCTGCGC‑TTTGCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTGCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCAACg                                                                                                                <  2:283002/250‑1 (MQ=255)                                                                                                                                 acctgATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCCCACC‑GCGCCTGGCCACGGcc                                                                                                             >  1:128006/5‑251 (MQ=255)                                                                                                                                              tATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGGTCACACC‑GCGCCTGGCCACGGCCCGGACGACTAtgt                                                                                                >  1:313630/1‑251 (MQ=255)                                                                                                                                                      ggTGAAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGCTCTGGTTGAAAGCGTAATGCAGCATATCGGCGTGAC‑CGACTACCACATTCTCAGCACAGCAAAAGGGG‑CGGCG‑CTTGAGATTCTGAGC‑TTTACCCCTCCGTTTATAGGCTTCGACGTTCCGGCAGTCCGCGGCGATACCGTTACCCTGTATTCCTGTGGCGGTGCTGTTC‑CACCCTCGCCTGGCCAccgacaggacgctcatggtctaggac                                                                                        >  2:116168/1‑226 (MQ=255)                                                                                                                                                                   ggTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCt                                                                           <  1:362690/251‑1 (MQ=255)                                                                                                                                                                   ggTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGccg                                                                           >  1:306341/1‑250 (MQ=255)                                                                                                                                                                    gTCAGGCCTTGGTTCTGGCGAAAGATCTGGTTGAAAGCGGAATGACGCGTATCGGCGTGAC‑CTATTACACCAGACTCAGCACGGTAAAATGTG‑CGGAG‑CTTGAGGTGCGTCGC‑GTTGCCCATCCGTGTATGGGCTTCGACGGTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTAGGTGATCGGTCAGAAATACTGCCTg                                                                          <  2:283388/251‑1 (MQ=255)                                                                                                                                                                           cGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CATGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGACTGGAAACCg                                                                   >  1:79210/1‑251 (MQ=255)                                                                                                                                                                                                ggTTTAAAGCGTAATGCAGCGTTTCGGAGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                  <  2:6508/247‑1 (MQ=255)                                                                                                                                                                                                          gTAATGCTGCGTATCGGCGGGAC‑CTATTACACCTATCTCAGCTCGGTAAATGGGG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAGCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTATCCCGGTTGGCCCGGACGGCACTTATCt                                    <  2:230837/251‑1 (MQ=255)                                                                                                                                                                                                                 aGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGc                             >  1:255810/1‑251 (MQ=255)                                                                                                                                                                                                                   cGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCAc                           <  1:273366/251‑1 (MQ=255)                                                                                                                                                                                                                          gCGTGAC‑CGATTACAGCATGCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGTCAATCCTCGGCGATCACGTTACCCTGGAGGCCGGTACCGGGGCCGTTCGCACC‑GCGCCTGGCCACGGCCCGGACTACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                    <  1:352618/251‑1 (MQ=255)                                                                                                                                                                                                                              gAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGc                >  1:229073/1‑251 (MQ=255)                                                                                                                                                                                                                                   gATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                                <  2:291884/104‑1 (MQ=255)                                                                                                                                                                                                                                   gATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGt                                                                                                                                                                >  1:291884/1‑104 (MQ=255)                                                                                                                                                                                                                                              tctcAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc  <  1:116168/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGC‑TTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACC‑GCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23030‑23515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑