Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 23,137 C→G A249A (GCC→GCG)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091323,1370CG87.3% 140.7 / 14.5 55A249A (GCC→GCG) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base G (27/21);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGT  >  NC_000913/22892‑23385
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
gcgcTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcg                                                                                                                                                                                                                                                        <  1:319312/251‑1 (MQ=255)
 cgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcgc                                                                                                                                                                                                                                                       >  1:292481/1‑251 (MQ=255)
    ttggCAAAATCATCGGCATCGGTCACCTGCACCAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAGTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGACGATCGATCAGGCTGAACTGAAAGCAAAATTTGCCGTAAGCAACGCTACCGGCCCAATCACGCTGGTTATATGGAACACTACGCCGTGGACTCGCCCTACGAACCgcgc                                                                                                                                                                                                                                                     >  2:288086/3‑250 (MQ=255)
       gCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAtc                                                                                                                                                                                                                                                 >  1:84339/1‑251 (MQ=255)
            atcatcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAt                                                                                                                                                                                                                                            <  1:380803/251‑1 (MQ=255)
                   gCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGAGCAATCTCTATTGCAca                                                                                                                                                                                                                                      >  2:78572/1‑249 (MQ=255)
                     aaCGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAcctg                                                                                                                                                                                                                                    >  1:295584/1‑248 (MQ=255)
                                                      cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCCCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCTCTGGTGCAGATCGACGGTCa                                                                                                                                                                                                  <  1:176412/251‑1 (MQ=255)
                                                      cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTc                                                                                                                                                                                                   <  1:381459/250‑1 (MQ=255)
                                                       aCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                                                                                                                                                                                                 <  1:242473/251‑1 (MQ=255)
                                                            tGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                                                                                                                                            >  1:123931/1‑251 (MQ=255)
                                                             gcggtGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTg                                                                                                                                                                                           <  1:28281/247‑1 (MQ=255)
                                                              cGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGACCGTGa                                                                                                                                                                                          <  2:80197/251‑1 (MQ=255)
                                                                ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCGGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTCTTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGAtt                                                                                                                                                                                        >  1:318516/1‑251 (MQ=255)
                                                                ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                                                                                                                                        >  1:283002/1‑251 (MQ=255)
                                                                    ctgacGTTCTGCGCTGGCGGAAGCGGCAGTTGAGTATTACGACAAAACGTCTCCGTCCATCGACGGTGCTTTTCAGGCGGTCGATCCGGATGCACTGACAGCAACATTTGCCGTAAGCAACGTTAACGGCCCTATCTCGCTGGTGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTTTTGCACCATATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGcagctcttct                                                                                                                                                                                      >  2:270170/5‑240 (MQ=255)
                                                                      gCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCACGCAATCTCTATTTCACCTGATTTAGACTATGCGCTGGTGCAGAACGACGGGCAGGCCGTGGTTCTGGCg                                                                                                                                                                                  >  1:193842/1‑251 (MQ=255)
                                                                              gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCACTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGa                                                                                                                                                                             <  1:78572/248‑1 (MQ=255)
                                                                                ccTGGAGGAAGCGGAATTTGAGTTTTACGACAAAACTTCTCCTGCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTTGCGAAAGAGCTgg                                                                                                                                                                        <  2:300165/250‑1 (MQ=255)
                                                                                     cggaagcggcagTTGAGTATTACGACAAAACTTCTCCGTCCATAGACGTTGCTTTTCAGGCGGTCGATCAGGATGCAATGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGAACCTGATTTCGACTATGCGCTGGTGAAGATCGACGTCCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                                                                                                                                    >  2:362690/1‑250 (MQ=255)
                                                                                         agcggaagTTGAGTATTACGACAAAACTTCTCCGTGCATCTACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCCCGCCGTTGACTCTGCCTGCCAACCGCGCAATCTCTATTGCAGCAGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                                                                                                                                               <  2:81508/251‑1 (MQ=255)
                                                                                            ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                                                                                                                             >  2:193650/1‑250 (MQ=255)
                                                                                               agTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                                                                                                                                         <  1:144319/251‑1 (MQ=255)
                                                                                                gTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCa                                                                                                                                                        <  1:113558/251‑1 (MQ=255)
                                                                                                  tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGAGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATATCTATTGCAACTGATTTCGACTATGCGCTGGTGCAGATCGCCGGACAGCCCGTGATTCTGGCGAGAGATCTGGTTGGAAGCGGAGTGCAGc                                                                                                                                                      >  2:850/1‑251 (MQ=255)
                                                                                                                aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc‑c                                                                                                                                       >  1:230837/1‑251 (MQ=255)
                                                                                                                aaaCTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc‑c                                                                                                                                       <  1:70087/251‑1 (MQ=255)
                                                                                                                         cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACAcc                                                                                                                              >  1:137541/1‑251 (MQ=255)
                                                                                                                         cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACAcc                                                                                                                              <  1:73616/251‑1 (MQ=255)
                                                                                                                                    gTTGATTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGAAACGTTAACGGCCCACTCTCGCTGGGGATCTGGACCACTACGACGTGGCCTCTGCCTGCGAACCTCGCAATCTCTATTGCACCTTATTTCGACTATGCGCTGGCGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGAGTAATGCAGCTTCTCGGCGTAGC‑CGATTaccacaatttcgacac                                                                                                                   >  2:25393/1‑237 (MQ=255)
                                                                                                                                      tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                                                                                                                                           <  1:293802/226‑1 (MQ=255)
                                                                                                                                      tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGagatcggaagagcgacgggtcggg                                                                                                                   >  2:293802/1‑227 (MQ=255)
                                                                                                                                          tttcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaa                                                                                                              <  1:211986/247‑1 (MQ=255)
                                                                                                                                            tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTa                                                                                                                                  >  1:207447/2‑228 (MQ=255)
                                                                                                                                            tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTa                                                                                                                                  <  2:207447/227‑1 (MQ=255)
                                                                                                                                              aGGCGGTCGATCAGGGTGCACTGAAAGCAAAATTTGCCGTACGCAACGTTAACCGCCCAAGCTCGCTGGTGCTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTGTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaaaatgt                                                                                                         <  1:346754/251‑4 (MQ=255)
                                                                                                                                                  ggTCGCTCAGGATGCACTGAAAGCAAAATTTGCCGTACGCAACGTTAACGGCCCAATCTCGCTGTTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTACACCTGATTTCGACTCTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCGTGCGACATATCTGTTTGAAGAAGGAATACAGAGGATCGGCGTAAC‑CGATTCCACCATtctcaacacgtaaaaaggagcgg                                                                                                     >  2:225540/2‑232 (MQ=255)
                                                                                                                                                   gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑Cggg                                                                                                   >  1:6508/1‑250 (MQ=255)
                                                                                                                                                     cGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑c                                                                                                <  1:126546/251‑1 (MQ=255)
                                                                                                                                                       aTCAGGATGCACTGAAAGCAAAATTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAATATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑Ctt                                                                                              <  1:170099/251‑1 (MQ=255)
                                                                                                                                                          aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTg                                                                                             >  1:359574/1‑249 (MQ=255)
                                                                                                                                                           ggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGAAATGCAGCGGATCGGCGTGAC‑CGATTACACCATTTTCAGCACGGTAACAGGTG‑CGGAG‑CTTGAgt                                                                                          >  2:62424/1‑250 (MQ=255)
                                                                                                                                                                cACTGAAAGCAAGATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAACACGGTAAAAGGTG‑CGGAG‑GTTGAGgtgctg                                                                                     >  1:259725/1‑251 (MQ=255)
                                                                                                                                                                   tGAAAGCAAAATTTCCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTgcgc                                                                                  >  1:311499/1‑251 (MQ=255)
                                                                                                                                                                    gAAAGCAAAATTTGCCGGAAGCAACGGTAACGGCTCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACGATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGAC‑CTATTACACCAGTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGGTGCTgcg                                                                                   <  2:378096/249‑1 (MQ=255)
                                                                                                                                                                         caaaattttccgatatcaactgtAACGGCCCAATCTCGCTGGTGATCTGGACCTCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGAGTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGGTTTAca                                                                            <  2:157882/229‑2 (MQ=255)
                                                                                                                                                                                 tccGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGt                                                                     <  2:168669/249‑1 (MQ=255)
                                                                                                                                                                                    gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGTTTAt                                                                 <  1:103580/251‑1 (MQ=255)
                                                                                                                                                                                          aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑AGATTAAACCATTCTCCACACGGAAAAAGGTGACGG‑G‑CTTGAGTTGCTTCGCTTTACCCATCCGTTTATGGGCtt                                                           >  2:273366/1‑251 (MQ=255)
                                                                                                                                                                                           aCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTc                                                          <  1:368873/251‑1 (MQ=255)
                                                                                                                                                                                            cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTACAAGGGG‑CGGAG‑ATTGAGTTGCTGCGCTTTACCCATCCGTTTATGGACTTCg                                                         >  1:42938/1‑251 (MQ=255)
                                                                                                                                                                                                        aaTCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCCTTCTCAGAACGGTAAAAGGAG‑CGGGG‑TTTGAGATGCCGCGCAGTACCCATCCGTTTATGGGCCTCGACGTACCGGcc                                              >  2:145304/1‑249 (MQ=255)
                                                                                                                                                                                                           ctcGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGATAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGGAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATcc                                          >  1:84614/1‑251 (MQ=255)
                                                                                                                                                                                                              gCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGAGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGTCGCAACGCG‑TTACAACATTCTCAGCACGGAAAAAGG‑G‑ACGAGATTTGTGTTGGTGGGCTTTACCCATCCGTTTATGAGCtttggcggtctggaactacacg                                       >  2:303397/1‑231 (MQ=255)
                                                                                                                                                                                                                                   gCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATg                  <  1:93284/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   gccAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGt  >  1:189423/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
GCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGT  >  NC_000913/22892‑23385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑