Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,116	C→T	T242T (ACC→ACT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,116	0	C	T	87.9%	135.6 / 7.3	58	T242T (ACC→ACT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base T (29/22);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

AACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCG  >  NC_000913/22870‑23348                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            aaCTGAAGCCCACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCAAATCTCGCTGGTGATCTGGACCACTACGc                                                                                                                                                                                                                                         >  1:94221/1‑251 (MQ=255)          cAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGActc                                                                                                                                                                                                                                <  1:10627/251‑1 (MQ=255)             catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGCCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                                                                                             >  1:367100/1‑251 (MQ=255)                catcCGTGCGCAGGGAAAAATCATCGGCAACAGGCTCCTGCACAAGTGCGCGAAGCC‑GTTACACAGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTCTTACGCAAAAACTTCTCCGGCCATCGACGTTGCTTTTCAGGCGGTCGATTAGGATGCACTGAAAGCAAAATTTGTCGTAATCAACGTTAACGGCCCAATCTCGCTGGTTATCTGTACCACTACGCCGTGGACTctgcatg                                                                                                                                                                                                                          <  2:369210/251‑4 (MQ=255)                       gcgcTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcg                                                                                                                                                                                                                   <  1:319312/251‑1 (MQ=255)                        cgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcgc                                                                                                                                                                                                                  >  1:292481/1‑251 (MQ=255)                           ttggCAAAATCATCGGCATCGGTCACCTGCACCAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAGTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGACGATCGATCAGGCTGAACTGAAAGCAAAATTTGCCGTAAGCAACGCTACCGGCCCAATCACGCTGGTTATATGGAACACTACGCCGTGGACTCGCCCTACGAACCgcgc                                                                                                                                                                                                                >  2:288086/3‑250 (MQ=255)                              gCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAtc                                                                                                                                                                                                            >  1:84339/1‑251 (MQ=255)                                   atcatcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAt                                                                                                                                                                                                       <  1:380803/251‑1 (MQ=255)                                          gCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGAGCAATCTCTATTGCAca                                                                                                                                                                                                 >  2:78572/1‑249 (MQ=255)                                            aaCGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAcctg                                                                                                                                                                                               >  1:295584/1‑248 (MQ=255)                                                                              cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCCCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCTCTGGTGCAGATCGACGGTCa                                                                                                                                                             <  1:176412/251‑1 (MQ=255)                                                                              cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTc                                                                                                                                                              <  1:381459/250‑1 (MQ=255)                                                                               aCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                                                                                                                                                            <  1:242473/251‑1 (MQ=255)                                                                                    tGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                                                                                                       >  1:123931/1‑251 (MQ=255)                                                                                     gcggtGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTg                                                                                                                                                      <  1:28281/247‑1 (MQ=255)                                                                                      cGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGACCGTGa                                                                                                                                                     <  2:80197/251‑1 (MQ=255)                                                                                        ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCGGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTCTTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGAtt                                                                                                                                                   >  1:318516/1‑251 (MQ=255)                                                                                        ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                                                                                                   >  1:283002/1‑251 (MQ=255)                                                                                            ctgacGTTCTGCGCTGGCGGAAGCGGCAGTTGAGTATTACGACAAAACGTCTCCGTCCATCGACGGTGCTTTTCAGGCGGTCGATCCGGATGCACTGACAGCAACATTTGCCGTAAGCAACGTTAACGGCCCTATCTCGCTGGTGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTTTTGCACCATATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGcagctcttct                                                                                                                                                 >  2:270170/5‑240 (MQ=255)                                                                                              gCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCACGCAATCTCTATTTCACCTGATTTAGACTATGCGCTGGTGCAGAACGACGGGCAGGCCGTGGTTCTGGCg                                                                                                                                             >  1:193842/1‑251 (MQ=255)                                                                                                      gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCACTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGa                                                                                                                                        <  1:78572/248‑1 (MQ=255)                                                                                                        ccTGGAGGAAGCGGAATTTGAGTTTTACGACAAAACTTCTCCTGCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTTGCGAAAGAGCTgg                                                                                                                                   <  2:300165/250‑1 (MQ=255)                                                                                                             cggaagcggcagTTGAGTATTACGACAAAACTTCTCCGTCCATAGACGTTGCTTTTCAGGCGGTCGATCAGGATGCAATGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGAACCTGATTTCGACTATGCGCTGGTGAAGATCGACGTCCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                                                                                               >  2:362690/1‑250 (MQ=255)                                                                                                                 agcggaagTTGAGTATTACGACAAAACTTCTCCGTGCATCTACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCCCGCCGTTGACTCTGCCTGCCAACCGCGCAATCTCTATTGCAGCAGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                                                                                                          <  2:81508/251‑1 (MQ=255)                                                                                                                    ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                                                                                        >  2:193650/1‑250 (MQ=255)                                                                                                                       agTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                                                                                                    <  1:144319/251‑1 (MQ=255)                                                                                                                        gTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCa                                                                                                                   <  1:113558/251‑1 (MQ=255)                                                                                                                          tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGAGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATATCTATTGCAACTGATTTCGACTATGCGCTGGTGCAGATCGCCGGACAGCCCGTGATTCTGGCGAGAGATCTGGTTGGAAGCGGAGTGCAGc                                                                                                                 >  2:850/1‑251 (MQ=255)                                                                                                                                        aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc‑c                                                                                                  >  1:230837/1‑251 (MQ=255)                                                                                                                                        aaaCTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc‑c                                                                                                  <  1:70087/251‑1 (MQ=255)                                                                                                                                                 cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACAcc                                                                                         >  1:137541/1‑251 (MQ=255)                                                                                                                                                 cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACAcc                                                                                         <  1:73616/251‑1 (MQ=255)                                                                                                                                                            gTTGATTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGAAACGTTAACGGCCCACTCTCGCTGGGGATCTGGACCACTACGACGTGGCCTCTGCCTGCGAACCTCGCAATCTCTATTGCACCTTATTTCGACTATGCGCTGGCGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGAGTAATGCAGCTTCTCGGCGTAGC‑CGATTaccacaatttcgacac                                                                              >  2:25393/1‑237 (MQ=255)                                                                                                                                                              tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                                                                                                      <  1:293802/226‑1 (MQ=255)                                                                                                                                                              tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGagatcggaagagcgacgggtcggg                                                                              >  2:293802/1‑227 (MQ=255)                                                                                                                                                                  tttcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaa                                                                         <  1:211986/247‑1 (MQ=255)                                                                                                                                                                    tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTa                                                                                             >  1:207447/2‑228 (MQ=255)                                                                                                                                                                    tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTa                                                                                             <  2:207447/227‑1 (MQ=255)                                                                                                                                                                      aGGCGGTCGATCAGGGTGCACTGAAAGCAAAATTTGCCGTACGCAACGTTAACCGCCCAAGCTCGCTGGTGCTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTGTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTaaaatgt                                                                    <  1:346754/251‑4 (MQ=255)                                                                                                                                                                          ggTCGCTCAGGATGCACTGAAAGCAAAATTTGCCGTACGCAACGTTAACGGCCCAATCTCGCTGTTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTACACCTGATTTCGACTCTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCGTGCGACATATCTGTTTGAAGAAGGAATACAGAGGATCGGCGTAAC‑CGATTCCACCATtctcaacacgtaaaaaggagcgg                                                                >  2:225540/2‑232 (MQ=255)                                                                                                                                                                           gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑Cggg                                                              >  1:6508/1‑250 (MQ=255)                                                                                                                                                                             cGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑c                                                           <  1:126546/251‑1 (MQ=255)                                                                                                                                                                               aTCAGGATGCACTGAAAGCAAAATTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAATATCTTGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑Ctt                                                         <  1:170099/251‑1 (MQ=255)                                                                                                                                                                                  aGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTg                                                        >  1:359574/1‑249 (MQ=255)                                                                                                                                                                                   ggATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGAAATGCAGCGGATCGGCGTGAC‑CGATTACACCATTTTCAGCACGGTAACAGGTG‑CGGAG‑CTTGAgt                                                     >  2:62424/1‑250 (MQ=255)                                                                                                                                                                                        cACTGAAAGCAAGATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAACACGGTAAAAGGTG‑CGGAG‑GTTGAGgtgctg                                                >  1:259725/1‑251 (MQ=255)                                                                                                                                                                                           tGAAAGCAAAATTTCCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTgcgc                                             >  1:311499/1‑251 (MQ=255)                                                                                                                                                                                            gAAAGCAAAATTTGCCGGAAGCAACGGTAACGGCTCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACTTGATTTCGACGATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGAC‑CTATTACACCAGTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGGTGCTgcg                                              <  2:378096/249‑1 (MQ=255)                                                                                                                                                                                                 caaaattttccgatatcaactgtAACGGCCCAATCTCGCTGGTGATCTGGACCTCTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGAGTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGGTTTAca                                       <  2:157882/229‑2 (MQ=255)                                                                                                                                                                                                         tccGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGt                                <  2:168669/249‑1 (MQ=255)                                                                                                                                                                                                            gTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGTTTAt                            <  1:103580/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑AGATTAAACCATTCTCCACACGGAAAAAGGTGACGG‑G‑CTTGAGTTGCTTCGCTTTACCCATCCGTTTATGGGCtt                      >  2:273366/1‑251 (MQ=255)                                                                                                                                                                                                                   aCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTAAAAGGTG‑CGGAG‑CTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTc                     <  1:368873/251‑1 (MQ=255)                                                                                                                                                                                                                    cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCAGCACGGTACAAGGGG‑CGGAG‑ATTGAGTTGCTGCGCTTTACCCATCCGTTTATGGACTTCg                    >  1:42938/1‑251 (MQ=255)                                                                                                                                                                                                                                aaTCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGAGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCCTTCTCAGAACGGTAAAAGGAG‑CGGGG‑TTTGAGATGCCGCGCAGTACCCATCCGTTTATGGGCCTCGACGTACCGGcc         >  2:145304/1‑249 (MQ=255)                                                                                                                                                                                                                                   ctcGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGATAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGGAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATcc     >  1:84614/1‑251 (MQ=255)                                                                                                                                                                                                                                      gCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGAGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGTCGCAACGCG‑TTACAACATTCTCAGCACGGAAAAAGG‑G‑ACGAGATTTGTGTTGGTGGGCTTTACCCATCCGTTTATGAGCtttggcggtctggaactacacg  >  2:303397/1‑231 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            AACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAC‑CGATTACACCATTCTCGGCACGGTAAAAGGTG‑CGGAG‑CTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCG  >  NC_000913/22870‑23348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑