Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,032	C→T	F214F (TTC→TTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,032	0	C	T	86.5%	115.1 / 7.0	52	F214F (TTC→TTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base T (24/21);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AGGTTGACGGTCAACGCAAAG‑ACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACG  >  NC_000913/22788‑23275                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aGGTTGACGGTCAACGCAAAG‑ACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCTCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGc                                                                                                                                                                                                                                                >  1:81508/1‑250 (MQ=255)            cAACGCAAAGTACTT‑ATCCGGCTGGGCTTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGAAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAgg                                                                                                                                                                                                                                     <  2:145386/250‑1 (MQ=255)                  aaaG‑AATTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACt                                                                                                                                                                                                                              >  1:300165/1‑251 (MQ=255)                   aaagtccttATCCGTCTGGGCGTGCTGGGCGCCTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACt                                                                                                                                                                                                                              <  2:66356/244‑1 (MQ=255)                     g‑ACTTTATCCGTCTGGGCGTGCTGGGCGACGGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCATGCGGTCGATCAGGATGCACTGaa                                                                                                                                                                                                                           <  1:41255/251‑1 (MQ=255)                               cGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAAtt                                                                                                                                                                                                                  >  1:168669/1‑251 (MQ=255)                                gTCTGGGGGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACCGCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGTACAAAGGCGCGAAGCCGGTA‑CCCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTACATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACGGAAAGCAAAAtgt                                                                                                                                                                                                                 <  2:86392/251‑3 (MQ=255)                                gTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGAATTACGCCAAAACTTCTCCGTCCATCGAAGTTGCTTTTCAGGCGGTAGCTCAGGATGCACTGCAAGCAAAAttt                                                                                                                                                                                                                 >  2:319312/1‑251 (MQ=255)                                             gggCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACGGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCaa                                                                                                                                                                                                      <  1:232271/249‑1 (MQ=255)                                               gCGACTGGTCGCACCCGTATCTTACCATGGACTTAAAAACTGAAGCCAACATCATACGCGCGCTTGGAAAAATCATCGGCAACGGTCACCGGCACAAAGGCTCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGTAGGCACTGAAAGCAAAATTTGCCGTAAGCAACggt                                                                                                                                                                                                  <  2:91329/251‑3 (MQ=255)                                                     ggTCTCACCCGTACCTTACCATGGACTTAAAACCTGAAGCCACCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTTCGCTGGCGGAAGCGGAAGTGGAGTATTACGACAAAACTTCTCCGTACATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACg                                                                                                                                                                                              <  2:308637/249‑1 (MQ=255)                                                        cGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCGGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTGGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGGAAGCAACGTTAACGGCCCa                                                                                                                                                                                         <  1:39459/251‑1 (MQ=255)                                                                    tGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                             >  2:73616/1‑250 (MQ=255)                                                                    tGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                             >  1:378096/1‑250 (MQ=255)                                                                      aCCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtgat                                                                                                                                                                           >  1:310785/1‑248 (MQ=255)                                                                                 cAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGaccact                                                                                                                                                                 <  1:113525/250‑2 (MQ=255)                                                                                    aaCTGAAGCCCACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCAAATCTCGCTGGTGATCTGGACCACTACGc                                                                                                                                                             >  1:94221/1‑251 (MQ=255)                                                                                             cAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGActc                                                                                                                                                    <  1:10627/251‑1 (MQ=255)                                                                                                catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGCCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                 >  1:367100/1‑251 (MQ=255)                                                                                                   catcCGTGCGCAGGGAAAAATCATCGGCAACAGGCTCCTGCACAAGTGCGCGAAGCC‑GTTACACAGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTCTTACGCAAAAACTTCTCCGGCCATCGACGTTGCTTTTCAGGCGGTCGATTAGGATGCACTGAAAGCAAAATTTGTCGTAATCAACGTTAACGGCCCAATCTCGCTGGTTATCTGTACCACTACGCCGTGGACTctgcatg                                                                                                                                              <  2:369210/251‑4 (MQ=255)                                                                                                          gcgcTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcg                                                                                                                                       <  1:319312/251‑1 (MQ=255)                                                                                                           cgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTAGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACcgc                                                                                                                                      >  1:292481/1‑251 (MQ=255)                                                                                                              ttggCAAAATCATCGGCATCGGTCACCTGCACCAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTAGTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGACGATCGATCAGGCTGAACTGAAAGCAAAATTTGCCGTAAGCAACGCTACCGGCCCAATCACGCTGGTTATATGGAACACTACGCCGTGGACTCGCCCTACGAACCgcgc                                                                                                                                    >  2:288086/3‑250 (MQ=255)                                                                                                                 gCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAtc                                                                                                                                >  1:84339/1‑251 (MQ=255)                                                                                                                      atcatcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAt                                                                                                                           <  1:380803/251‑1 (MQ=255)                                                                                                                             gCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGAGCAATCTCTATTGCAca                                                                                                                     >  2:78572/1‑249 (MQ=255)                                                                                                                               aaCGGTCACCTGCACAAAGGCGCGAAGCCGGTA‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAcctg                                                                                                                   >  1:295584/1‑248 (MQ=255)                                                                                                                                                                 cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCCCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCTCTGGTGCAGATCGACGGTCa                                                                                 <  1:176412/251‑1 (MQ=255)                                                                                                                                                                 cACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTc                                                                                  <  1:381459/250‑1 (MQ=255)                                                                                                                                                                  aCTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                                                                                <  1:242473/251‑1 (MQ=255)                                                                                                                                                                       tGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                           >  1:123931/1‑251 (MQ=255)                                                                                                                                                                        gcggtGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTg                                                                          <  1:28281/247‑1 (MQ=255)                                                                                                                                                                         cGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGACCGTGa                                                                         <  2:80197/251‑1 (MQ=255)                                                                                                                                                                           ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCGGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTCTTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCCGGCCGTGAtt                                                                       >  1:318516/1‑251 (MQ=255)                                                                                                                                                                           ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                       >  1:283002/1‑251 (MQ=255)                                                                                                                                                                               ctgacGTTCTGCGCTGGCGGAAGCGGCAGTTGAGTATTACGACAAAACGTCTCCGTCCATCGACGGTGCTTTTCAGGCGGTCGATCCGGATGCACTGACAGCAACATTTGCCGTAAGCAACGTTAACGGCCCTATCTCGCTGGTGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTTTTGCACCATATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGcagctcttct                                                                     >  2:270170/5‑240 (MQ=255)                                                                                                                                                                                 gCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCACGCAATCTCTATTTCACCTGATTTAGACTATGCGCTGGTGCAGAACGACGGGCAGGCCGTGGTTCTGGCg                                                                 >  1:193842/1‑251 (MQ=255)                                                                                                                                                                                         gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGACTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCACTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGa                                                            <  1:78572/248‑1 (MQ=255)                                                                                                                                                                                           ccTGGAGGAAGCGGAATTTGAGTTTTACGACAAAACTTCTCCTGCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTTGCGAAAGAGCTgg                                                       <  2:300165/250‑1 (MQ=255)                                                                                                                                                                                                cggaagcggcagTTGAGTATTACGACAAAACTTCTCCGTCCATAGACGTTGCTTTTCAGGCGGTCGATCAGGATGCAATGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGAACCTGATTTCGACTATGCGCTGGTGAAGATCGACGTCCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                   >  2:362690/1‑250 (MQ=255)                                                                                                                                                                                                    agcggaagTTGAGTATTACGACAAAACTTCTCCGTGCATCTACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCCCGCCGTTGACTCTGCCTGCCAACCGCGCAATCTCTATTGCAGCAGATTTCGACTATGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                              <  2:81508/251‑1 (MQ=255)                                                                                                                                                                                                       ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTa                                            >  2:193650/1‑250 (MQ=255)                                                                                                                                                                                                          agTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGc                                        <  1:144319/251‑1 (MQ=255)                                                                                                                                                                                                           gTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCa                                       <  1:113558/251‑1 (MQ=255)                                                                                                                                                                                                             tGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGAGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAGATCTGGACAACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATATCTATTGCAACTGATTTCGACTATGCGCTGGTGCAGATCGCCGGACAGCCCGTGATTCTGGCGAGAGATCTGGTTGGAAGCGGAGTGCAGc                                     >  2:850/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                       >  1:230837/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaCTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                       <  1:70087/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc              <  1:73616/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc              >  1:137541/1‑251 (MQ=255)                                                                                                                                                                                                                                               gTTGATTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGAAACGTTAACGGCCCACTCTCGCTGGGGATCTGGACCACTACGACGTGGCCTCTGCCTGCGAACCTCGCAATCTCTATTGCACCTTATTTCGACTATGCGCTGGCGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGAGTAATGCAGCTTCTCGGCGTAGCCGATTaccacaatttcgacac   >  2:25393/1‑237 (MQ=255)                                                                                                                                                                                                                                                 tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                          <  1:293802/226‑1 (MQ=255)                                                                                                                                                                                                                                                 tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGagatcggaagagcgacgggtcggg  >  2:293802/1‑227 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AGGTTGACGGTCAACGCAAAG‑ACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTT‑CACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACG  >  NC_000913/22788‑23275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑