Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,670	T→A	intergenic (+50/+141)	nhaR → / ← insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,670	0	T	A	100.0%	44.2 / NA	18	intergenic (+50/+141)	nhaR/insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/11);  total (7/11)

TGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTAT  >  NC_000913/19440‑19814                                                                                                                                                                                                                                        |                                                                                                                                                 tGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCgg                                                                                                                                <  1:94948/250‑1 (MQ=255)      cAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatt                                                                                                                          <  1:85700/251‑1 (MQ=255)                           ttgaTGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGATATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACAt                                                                                                       <  1:251904/246‑1 (MQ=255)                               tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTc                                                                                                 >  1:266122/1‑251 (MQ=255)                                      aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCAGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTCGGACAGCGGCGAGTTAATCTGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCCCGATTTTGACTCGGCTCATTATTGTCCCGATTTAGACAATATTTCCATATGt                                                                                          >  2:201590/1‑251 (MQ=255)                                              cGTAGAAATTGTGCGCGTCGAGAATTTTATGGATGAGTACCATGCTATTTTTGCTGAGCGTATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGTATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTTGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGATCATTATTTGCCTGCTTGAGACATTATTTCCATATGTACGCggg                                                                                   <  1:79690/250‑1 (MQ=255)                                                                    aaTGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGAATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATGCGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTAGGGGAGAGAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCAATATGGACGCGGGCGAATAAATAGAGGAATCTGAtt                                                            >  2:210806/1‑251 (MQ=255)                                                                                      cATGCTATTTTTTCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTAGTCTGCGTTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATg                                          <  2:166177/251‑1 (MQ=255)                                                                                                  gCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCACTACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACAACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGCTGCTGAAAAGagt                               >  2:134627/1‑250 (MQ=255)                                                                                                              atTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                  >  1:12504/1‑248 (MQ=255)                                                                                                                         ggcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCTGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactgga       <  1:353701/251‑15 (MQ=255)                                                                                                                          gcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggat      <  1:201590/251‑16 (MQ=255)                                                                                                                           cggTACATCGAACTTGCAATACGGATTAGTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGGAAAGAGTAGTAATTGCttttaccatactggatt     <  2:119880/251‑17 (MQ=255)                                                                                                                             gTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGTGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttc   <  1:134206/251‑19 (MQ=255)                                                                                                                              tACAGCGATTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGCTTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGAGGATGCTGAAAAGAGGAGTAAtagatcgtaagatcgtcgtggagg  >  2:224728/1‑228 (MQ=255)                                                                                                                              tACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAAt                         <  1:224728/228‑1 (MQ=255)                                                                                                                                                                                                           aaGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactgga       >  1:278609/1‑155 (MQ=255)                                                                                                                                                                                                           aaGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactgga       <  2:278609/169‑15 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                 TGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTAT  >  NC_000913/19440‑19814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑