Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,613	T→C	V300A (GTG→GCG)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,613	0	T	C	100.0%	50.2 / NA	22	V300A (GTG→GCG)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

AGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑A‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTAT  >  NC_000913/19382‑19814                                                                                                                                                                                                                                        |                                                                                                                                                                                                            aGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTccc                                                                                                                                                                                          >  1:253904/1‑251 (MQ=255)    gtgatgaTGCCGCGTTGTTGAAAGCTTTTGGTGAGCTGCAAAATGCAATCTCCGTTGACCCAACGTTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTATAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATGCTGCGCTTATTAGTCCAGCGGCGCGTTAATCGGCAACTcaccc                                                                                                                                                                                        <  2:152441/249‑1 (MQ=255)                   tgatgaAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATCCGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTgggg                                                                                                                                                                       >  2:94948/1‑251 (MQ=255)                       aaaaGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCTACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTTGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAACCGGCACCTCCCCCCA‑AAGTTAAGGTGTGGgaga                                                                                                                                                                   <  2:163588/250‑1 (MQ=255)                                        tgcacctttcactCTTCGTTGCCCCAACGCGTTATGCCTTTGACTTTGATGCCGGAAAAACTGGTGTAGTAATTGTACGCGTCGAGTATTTGATGGTATAGTACCATTGTATTTTTGCTGAGCGTATGATTCAGCAGCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCTCTTTTTAGGCCAGAGGCGCGTTAATCGGCACTTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACAt                                                                                                                                                   <  2:209940/238‑1 (MQ=255)                                         gCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCC‑CCAAGTTAAGGTGGGGGCGATAGATTAGTTGTACAta                                                                                                                                                  >  1:68260/1‑249 (MQ=255)                                                           tGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCgg                                                                                                                                <  1:94948/250‑1 (MQ=255)                                                                cAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCatt                                                                                                                          <  1:85700/251‑1 (MQ=255)                                                                                     ttgaTGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGATATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACAt                                                                                                       <  1:251904/246‑1 (MQ=255)                                                                                         tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTc                                                                                                 >  1:266122/1‑251 (MQ=255)                                                                                                aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCAGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTCGGACAGCGGCGAGTTAATCTGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCCCGATTTTGACTCGGCTCATTATTGTCCCGATTTAGACAATATTTCCATATGt                                                                                          >  2:201590/1‑251 (MQ=255)                                                                                                        cGTAGAAATTGTGCGCGTCGAGAATTTTATGGATGAGTACCATGCTATTTTTGCTGAGCGTATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGTATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTTGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGATCATTATTTGCCTGCTTGAGACATTATTTCCATATGTACGCggg                                                                                   <  1:79690/250‑1 (MQ=255)                                                                                                                              aaTGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGAATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATGCGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTAGGGGAGAGAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCAATATGGACGCGGGCGAATAAATAGAGGAATCTGAtt                                                            >  2:210806/1‑251 (MQ=255)                                                                                                                                                cATGCTATTTTTTCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTAGTCTGCGTTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATg                                          <  2:166177/251‑1 (MQ=255)                                                                                                                                                            gCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCACTACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACAACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGCTGCTGAAAAGagt                               >  2:134627/1‑250 (MQ=255)                                                                                                                                                                        atTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                  >  1:12504/1‑248 (MQ=255)                                                                                                                                                                                   ggcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCTGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactgga       <  1:353701/251‑15 (MQ=255)                                                                                                                                                                                    gcggTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggat      <  1:201590/251‑16 (MQ=255)                                                                                                                                                                                     cggTACATCGAACTTGCAATACGGATTAGTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGGAAAGAGTAGTAATTGCttttaccatactggatt     <  2:119880/251‑17 (MQ=255)                                                                                                                                                                                       gTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGTGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttc   <  1:134206/251‑19 (MQ=255)                                                                                                                                                                                        tACAGCGATTCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGCTTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGAGGATGCTGAAAAGAGGAGTAAtagatcgtaagatcgtcgtggagg  >  2:224728/1‑228 (MQ=255)                                                                                                                                                                                        tACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCA‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAAt                         <  1:224728/228‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                            AGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑A‑AAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTAT  >  NC_000913/19382‑19814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑