Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 19,014 C→T G100G (GGC→GGT)  nhaR → DNA‑binding transcriptional activator NhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091319,0140CT97.7% 123.5 / ‑4.3 43G100G (GGC→GGT) nhaRDNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (17/25);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

AAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTGAGTTTCTGGTGTACAAATCCACCACC  >  NC_000913/18769‑19256
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
aaaGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGc                                                                                                                                                                                                                                               >  1:11656/1‑251 (MQ=255)
  agaagGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGCTATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTTGTGTGGCTg                                                                                                                                                                                                                                             >  2:151033/1‑251 (MQ=255)
       gTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGAATCGAGCGCTGGAAGAGCTCCTGCAAGGCAAATTATGTAAACGCAAGGGACGTGGTCACGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCa                                                                                                                                                                                                                                        <  2:11656/251‑1 (MQ=255)
             tGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTAAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAAATATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTcc                                                                                                                                                                                                                                  >  1:231637/1‑251 (MQ=255)
              ggTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCACAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTcc                                                                                                                                                                                                                                  >  1:18466/1‑250 (MQ=255)
              ggTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCa                                                                                                                                                                                                                                 <  1:338480/251‑1 (MQ=255)
                     gcAGCGGAGGCTCTTTATCTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGTCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGTTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCt                                                                                                                                                                                                                          <  1:358748/251‑1 (MQ=255)
                      cAGCGGAGGCGCTTTAATTAACTACACAAACCATTACCGGACAGATTAGAGCGCTGAAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATTCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAAAATCCATTTTATTGTTTGACGTTGGTGGGGCGGGTGCCCTTTCCACACGcatg                                                                                                                                                                                                                         >  2:42197/1‑248 (MQ=255)
                                   ttatttaACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGt                                                                                                                                                                                                              <  1:133666/249‑1 (MQ=255)
                                       ttaACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTa                                                                                                                                                                                                         >  1:204416/1‑250 (MQ=255)
                                       ttaACTCCACAAACCATTACCGGACAGATTCAAGAGCTGGAAGAGCGACTGCAAGACAAATTATTTAAACGCAAAAGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGGCTATCGATATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTCTTGTGTGACGTTGGTGTGGCTGATGCACTTTCCAAACAACTGGTCAGTAGCGTAcgta                                                                                                                                                                                                         >  2:56870/1‑247 (MQ=255)
                                                    ccATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGGTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTa                                                                                                                                                                                           <  2:204416/251‑1 (MQ=255)
                                                         aCCGGACAGGTTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAgg                                                                                                                                                                                      >  1:323734/1‑251 (MQ=255)
                                                                    tCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCAtt                                                                                                                                                                            <  1:54325/250‑1 (MQ=255)
                                                                                           aaGGCAAATGATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccacc                                                                                                                                                    <  1:253933/251‑1 (MQ=255)
                                                                                                 aaTTATTTAACCGCAAGGGACGTGGTCTCGCACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATTCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTTTTTGACGTTGGTGTGGCTGATGCACATTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGGGGTGTAAGGCGAGCCCATTCATCTACGCTGCTCTGTATCCACCCACGa                                                                                                                                               >  2:188410/1‑250 (MQ=255)
                                                                                                           aCGCAAGGGACGGGGTCTCGAACCCAGAGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAAGCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTgg                                                                                                                                    <  2:264370/251‑1 (MQ=255)
                                                                                                                  ggACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCTCCCACGAAATGCTGCTGGAGCAAtt                                                                                                                             <  1:351476/251‑1 (MQ=255)
                                                                                                                             ctaaCCCAGCGAGCTTGGATAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTTTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCa                                                                                                                    <  1:30542/247‑1 (MQ=255)
                                                                                                                               aaCCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATaa                                                                                                                 >  1:232419/1‑250 (MQ=255)
                                                                                                                                aCCCAGCGAACTGGGAGAACTGGTCTAACGCTATGCCGATAAAATGTTCAACTTAAGACAGGAAATGCTGGATATTGTGAACTATCGCAAAGAAGCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTGCCAAACGCCTGGTCAGTAGCGTACTGAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGTATAAAc                                                                                                               <  2:121310/251‑1 (MQ=255)
                                                                                                                                         gCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCTATTAAGTCAGCATAAACTGGATATGa                                                                                                      <  1:153557/251‑1 (MQ=255)
                                                                                                                                            tggAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGAt                                                                                                     <  1:331397/248‑1 (MQ=255)
                                                                                                                                              gagaACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCAtt                                                                                                 <  1:304201/251‑1 (MQ=255)
                                                                                                                                                  aCTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGAAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCATGAAATGCTGCTGGAGCAATTAAGTCAGCCTAAACTGAATATGATCATTTCTg                                                                                             >  1:217009/1‑251 (MQ=255)
                                                                                                                                                    tGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTc                                                                                                                                                                     >  1:145136/1‑177 (MQ=255)
                                                                                                                                                    tGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTc                                                                                                                                                                     <  2:145136/177‑1 (MQ=255)
                                                                                                                                                        cTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTc                                                                                       >  1:321856/1‑251 (MQ=255)
                                                                                                                                                          aTCGCTATTGCGATAAAATGTTCACCTTAAGGCAGGATATGCAGGAAATTGTGAACTAGCGAAAATAATCCAATTTATTGTTTGACGTTGGTGTGGCTGAAGCACTATCCAAACGACTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGATCCCATTCATCTTCGTGGCTTCGAATCCGCACGTGATATGCTGCTGGAGCATGTAGATGAGCATAAGATGGATATGATCATTTctgtctgt                                                                                        <  2:282535/248‑1 (MQ=255)
                                                                                                                                                          aTCGCTATGCCGATAAAATGTTCACCATAAGCCAGGAAATGGTGTATCTGGTGAACGATCGCAATGAATCCGTCTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTTGCGTACGTAACGCCGCAGAGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACTCTCGAAATGCTGCTTGATCAATTAAGTCAGCATTAACTGGATATGATCATTTCTGACttaca                                                                                      <  2:123669/250‑5 (MQ=255)
                                                                                                                                                                 tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGAct                                                                              >  1:146991/1‑251 (MQ=255)
                                                                                                                                                                                cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGaa                                                                <  1:188410/250‑1 (MQ=255)
                                                                                                                                                                                cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGGTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAg                                                               <  2:146991/251‑1 (MQ=255)
                                                                                                                                                                                     tAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCt                                                           <  1:42197/250‑1 (MQ=255)
                                                                                                                                                                                           aGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTTGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTcc                                                    <  1:151033/251‑1 (MQ=255)
                                                                                                                                                                                                 tGCTGGATATTGTGAACGATCGCACAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAAAGCCTGGTCAGTAGCGTACTTAACTCCGCAGTGGTAGCAGCAGAGCCCATTCATCTTCGATGCTTAGTATCCACCCACGAAAAGCTGCTGGAGCTAGTACGACAGCATAAATTTGATATGATCATTTCTGACTGTCCGATAGACTATACGCAGCAGGTAAGCCTATTTTCCGTgtg                                               >  2:279088/1‑248 (MQ=255)
                                                                                                                                                                                                         atTGTGAACTATCGCAAAGAATCCATTTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCCAGCCCATTCATCTTCGCTGCTTCGAATCCTCCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCg                                       <  2:187373/250‑1 (MQ=255)
                                                                                                                                                                                                                   aTCGCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTg                            <  1:94367/251‑1 (MQ=255)
                                                                                                                                                                                                                      gCAAAGAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTGAGt                         <  1:64190/251‑1 (MQ=255)
                                                                                                                                                                                                                           gtctCCAATTTATTGCTTGACGGTGGTGGTGCTGATGTACTCTCCAATCGCCTGGTCAGTTGCGTACGTAACGTGGCAGTGGTATAAGGTTAGCCCTGTCAGCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCTGCATAAACTGGATATGATCATGTCTGACTGTTCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGACTGTGTCGTGAGTTTCTg                    <  2:137106/248‑1 (MQ=255)
                                                                                                                                                                                                                           gAATCCAATTTATTGTTTGACGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTGAGTTTCTg                    >  1:32631/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cGTTGGTGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGCAGGCGCGCCCATTCATATTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATCGACTCTACGCCGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGAGCGTTTCTGGTGGACAAATccacaccc  >  2:262241/1‑245 (MQ=255)
                                                                                                                                                                                                                                               cGTTGGTGTAGCTGATGCACTTTCCAAACGCCTGATCAGTAACGTACTTAACGCCGCAGTGGTAGACGGCGAGCACATTCATCTTCACTGCTTCGAATCCACCCACGAAATGCTGATGGAGCAATTAAGTCAGCATACACTCGCAATGATCATTTCTGAATGTCCGTTAGACTCTACACAGCGGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTGCGTTTCTGGAGTACAACaccaccacc  >  2:353701/1‑249 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
AAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGACAGATTCGAGCGCTGGAAGAGCGCCTGCAAGGCAAATTATTTAAACGCAAGGGACGTGGTCTCGAACCCAGCGAGCTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAACTGGATATGATCATTTCTGACTGTCCGATAGACTCTACGCAGCAGGAAGGCCTGTTCTCCGTGAGAATTGGCGAATGTGGCGTGAGTTTCTGGTGTACAAATCCACCACC  >  NC_000913/18769‑19256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑