Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 18,322 C→T G278G (GGC→GGT nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091318,3220CT96.3% 69.5 / ‑5.0 28G278G (GGC→GGTnhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (9/17);  minor base G (1/0);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

GGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGTGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGG  >  NC_000913/18089‑18568
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      
ggggtaCGCCGCGCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGGTCACGCAACCCTGGCGTGGGTAATTGTCTGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCtt                                                                                                                                                                                                                                       <  2:274786/246‑1 (MQ=255)
       gccgcACGAGCGTCTATATTCTGGTTGGCGTCGTGTTGTGGACTGCGGTGTTGCAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCCTGGGCGTTCTCCAGCGAAGCGCCTGGGGCCTGTGTTAAACCCGTTGTGGGCGTCTCTGATTTTGCCGCTTTTTGCATTTGCTAATGTGGACGTTTCACTTAAAGGTGTCACGCTGGATGGCTTTACCTcc                                                                                                                                                                                                                                >  2:124633/1‑251 (MQ=255)
              gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGc                                                                                                                                                                                                                         <  1:99714/251‑1 (MQ=255)
                             ggttgGCGTAGTGTTGTGGACTGTGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTTTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGatcat                                                                                                                                                                                                            <  1:262844/248‑1 (MQ=255)
                              gttgGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGc                                                                                                                                                                                                         >  1:65068/1‑251 (MQ=255)
                                                                   gggTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTg                                                                                                                                                                    <  1:147949/251‑1 (MQ=255)
                                                                                   ggCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGgcgc                                                                                                                                                    >  1:179129/1‑251 (MQ=255)
                                                                                            aaTTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTg                                                                                                                                            <  1:124633/250‑1 (MQ=255)
                                                                                              ttGTCGGATTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTTGCGCTGCGTTTGAAAc                                                                                                                                        >  2:163086/1‑251 (MQ=255)
                                                                                                 tCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGCTTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTg                                                                                                                                      >  1:76759/1‑251 (MQ=255)
                                                                                                                 tttGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGATTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCGTCTGCCTGAgg                                                                                                                      >  1:351223/1‑251 (MQ=255)
                                                                                                                  ttGAAAGAGAAGCATGGGCGGTCTCCAGCTAAGCGACTGTAGCAGTTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGATGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGTATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGAGTGGCAACCCGCTGGGGATTAGTGTGTTCTGCTGTTTGGCGCTTCGTTTGAAACTTGCGCATCTTCCTGa                                                                                                                        <  2:76759/248‑1 (MQ=255)
                                                                                                                    gAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTTCTCCCGTGGGTGTCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGaa                                                                                                                   <  1:264183/251‑1 (MQ=255)
                                                                                                                         agaAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGATTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACt                                                                                                              >  1:250243/1‑251 (MQ=255)
                                                                                                                          gaggCATGGGCTTTCTCCATCTAAGCTACTGGTGCTTGTGTTTCCCCCGTGGGTGGCTTATCTTATTTTGTCGCTGTTTGCTTTTTGTTATGCTGGCGTTTCACTGCAAGGTGTCACTCTGGATGGCTTGACCTCCTTTCTGCTTTTGGGGATCAGCTCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCAGCTTGTTGGTGCTGGGTTTTAAACTGTCGCTTCTGCCTGAGGGATCGACtt                                                                                                             <  2:268768/248‑1 (MQ=255)
                                                                                                                                   gCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACGCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACGTATCGGCaa                                                                                                     >  2:338480/1‑250 (MQ=255)
                                                                                                                                           cAGCGAAGCGACTGGAGCATGTGTTACACCCGCGGGAGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGTCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTACTCATCAACTTAtgg                                                                                              >  2:133666/1‑249 (MQ=255)
                                                                                                                                             gcgaagcgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTAtggcggt                                                                                          <  1:232294/251‑1 (MQ=255)
                                                                                                                                              ggaagcgaCTGGTGCATTTGTTACCCCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCGCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGGCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTAtggcggtg                                                                                         <  2:129482/250‑1 (MQ=255)
                                                                                                                                                 agcgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTgggg                                                                                      <  1:137060/251‑1 (MQ=255)
                                                                                                                                                   cgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGTGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTctgc                                                                                                                                                              >  1:358010/1‑177 (MQ=255)
                                                                                                                                                    gaCTTGAGCATGAGTTACACCCGTGGGTGGCGTATCTGATTTAGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGGGTCACGATGGCTGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGACTTGCTGCTTGGCAAACCGCTGGGGATTAGTCTGTTCTGATGGTTGGCACTGCGATTGAAACAGGCGCATGTGCCTGGGGGAACGACTTATCAGCATATTATGGCGGAggggttt                                                                                   >  2:285834/1‑248 (MQ=255)
                                                                                                                                                         gagcttgtgttttgccagggggtggCTTATCTTGTTTTGCCGCTAGTTGCAATTGCTAATGCTTGCGTTTCACGTGAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGTCATTGTGGATCATCGCGGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTGTGTGCTGCGGGTTTGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTAGCAGCAAATTATGGCGGTGGGGATCCtgt                                                                               <  2:32725/231‑1 (MQ=255)
                                                                                                                                                                    acACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTGGGGATCCtg                                                                                <  1:379706/237‑1 (MQ=255)
                                                                                                                                                                                                          cATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGt                             <  1:285834/251‑1 (MQ=255)
                                                                                                                                                                                                           aTTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGGTGGATGGCTTGACCTCCATGCTGCCATTGAGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTa                            <  1:215110/251‑1 (MQ=255)
                                                                                                                                                                                                             ttGCTAATGCTGGCGTTTCACGGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGTGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAg                           <  1:58575/250‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAGCGTAGATCCAGAACTGATTAACTgg  <  1:2817/251‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      
GGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGTGGTGGGGATCCTGTGCGGTATCGGTTTTACTATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGG  >  NC_000913/18089‑18568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: