Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,893 T→C A135A (GCT→GCC nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,8930TC87.1% 73.1 / ‑0.1 32A135A (GCT→GCCnhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (16/11);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

ACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGG  >  NC_000913/17682‑18136
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
aCGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGtt                                                                                                                                                                                                              <  1:273033/251‑1 (MQ=255)
       gATGGCGGAATTTTTCCTGTTAGTCGGACTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTCTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACAATGCCGATCCGATTACCCGCGAAGGGTGGGGGCTCCCGGCGGCTACTGACATGGCCTTTGCACTTGGTGTGTTGGCGCTGTTGGGAAGTCGGGTTCCGtt                                                                                                                                                                                                         >  1:69290/1‑249 (MQ=255)
              gTATTTTTCCTGTTAGTCGGTCTGGAAGTTCAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCCTTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGa                                                                                                                                                                                                 >  1:125816/1‑250 (MQ=255)
                         gTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGACCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGGTCCGTTAGCGCTGAAGATCTTTTTGa                                                                                                                                                                                     <  1:286020/251‑1 (MQ=255)
                            aGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGTAGCCGTGTTGCGTTATCGCTGAAGATCTTTTTGATgg                                                                                                                                                                                  >  2:258969/1‑251 (MQ=255)
                                        aGTTAAACTGGAACCGAGGCAAGTAGCGCTAGCCAGCGTGCGCCAGGCCGCATTGCCAGTTATCGCCGCTATTGGTGGGATGAGTGTGCCGGCATTACTCTATCTGGCTTTTAACTAGGCCGAACCGATTACCCGCGAAGGTTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGTACGTCGTGTTCCGTGAGCGCGGAAGATCATTTTGATGGCTCTGGCtagta                                                                                                                                                                      <  2:125816/251‑4 (MQ=255)
                                                  gaccttaTGCAAGGATCGCTAGCCAGCTTACTTGTGGTCGCATTTCCAGTTATCGCCGCCATTGGTGGGCTTATTGTTCCGGCATTACTCTATCTGGCCCTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCTGCTACTGACATTGCCTTTGCACTTTGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCTGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATc                                                                                                                                                             <  2:86017/244‑1 (MQ=255)
                                                        aTGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGc                                                                                                                                                      <  1:30785/251‑1 (MQ=255)
                                                          gCAAGGATCGCTAGCCAGCTTACGCCCGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGGTAGCGCTGAAGATCTTTTTGCTGGCTCTGGCTATTATCGGCGATCTTGGGGcac                                                                                                                                                    >  2:67205/1‑249 (MQ=255)
                                                            aaGGATCGCTAGCCAGCTTACGCGAGGCCGCATTTCCAGATATCGCCGCTGTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGAGTTGGCGATCCCGGCAGCTCCTGACATTGCCTTTGAACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCCATGAAGATCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGccgtc                                                                                                                                                  >  1:139831/1‑248 (MQ=255)
                                                              ggATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGTCGATCTTGAGGCcatcat                                                                                                                                                >  1:52636/1‑251 (MQ=255)
                                                                   gCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGCGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGATACTGCCATTGCCTTTGCACTTGGAGTGCGGGCGCGGTTTGGAAGTCGTGTTCCGTTAGCGCTGAGGATCTTTTTTATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTatc                                                                                                                                            >  2:203872/1‑250 (MQ=255)
                                                                                      gCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTATTCTACACTa                                                                                                                         >  1:17402/1‑250 (MQ=255)
                                                                                                cAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCg                                                                                                              <  1:67205/251‑1 (MQ=255)
                                                                                                    tATCGCCGCTATTGGTGGGACGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATg                                                                                                           <  1:108404/250‑1 (MQ=255)
                                                                                                     aTCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATgg                                                                                                          <  1:313442/250‑1 (MQ=255)
                                                                                                              aTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGAGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGATGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTgg                                                                                                >  1:343046/1‑251 (MQ=255)
                                                                                                                tggtggGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCATTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTCTTATCGTAGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTATCTTGGCg                                                                                              >  1:44450/1‑251 (MQ=255)
                                                                                                                         gatTGTTCCGGAATTAATCTATCTGGCCTTTAACTATGCCGATCCGCTTACCAGCGCAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGAAGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACAATAATGACTTATCGATGTCCTCTCTTGGCGTCGCGGCtt                                                                                     >  2:231511/1‑250 (MQ=255)
                                                                                                                                       taccttATCTGGCTTTTAACTCTGCAGATCCGATTACCCGCGTAGGGTTGGCGATCCCGGCGGCTACTGTCATTGCTGTTGCTCTTGGTGTACTGGCGCTGTTGGGAAGTTGTGTTCCGTTAGCGCTGAAGATCTTTTTTATGGCTCTGTCTCTTATCGACGATCTTGGGGCCATCATTCTCATCGCATTGTTCTACCCTAATGCCGTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGt                                                                        <  2:255254/245‑1 (MQ=255)
                                                                                                                                        aCTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAc                                                                      >  1:239124/1‑251 (MQ=255)
                                                                                                                                          tctATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTc                                                                    >  1:311070/1‑251 (MQ=255)
                                                                                                                                                        acgcttgccgttctgattcaccGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTTCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCt                                                      <  2:172678/231‑1 (MQ=255)
                                                                                                                                                        aaCTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCt                                                      >  1:33474/1‑251 (MQ=255)
                                                                                                                                                          cTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGATACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATATTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGACATCATTCTCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGTCGTCGCGGCTGTCGCAAATGCGGTACTCGCGGTTTTGACTCtgg                                                    >  2:337376/1‑250 (MQ=255)
                                                                                                                                                                 gattcgatTTCCCGCGAAGTGTGGGCTATCCCGGCGGGTTCTGACATTGCCTTTGCACTTTGTGTGTTGGCGCTGTTGGGAAGTCGTGTGACGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTGGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGTCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTTCTCGCGGTATTGAATCTGTttggtgt                                             <  2:247827/247‑2 (MQ=255)
                                                                                                                                                                      gatTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCt                                                      >  1:172678/1‑237 (MQ=255)
                                                                                                                                                                                                cggcggCTACTGCCATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGTCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTGTTGGATCTGTGTGGGGTACGCCGCACGGGCGt                              >  2:330551/1‑235 (MQ=255)
                                                                                                                                                                                                cggcggCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGt                              <  1:330551/235‑1 (MQ=255)
                                                                                                                                                                                                   gggcTACTGTCATTTCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTATCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCgta           <  1:207576/250‑2 (MQ=255)
                                                                                                                                                                                                      cTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTAcg                                          <  1:346079/217‑1 (MQ=255)
                                                                                                                                                                                                            aCATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTgg  >  1:268768/1‑251 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
ACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGG  >  NC_000913/17682‑18136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: