Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,830 T→C A114A (GCT→GCC nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,8300TC84.6% 57.5 / ‑0.5 26A114A (GCT→GCCnhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (15/7);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTC  >  NC_000913/17595‑18070
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
gATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgat                                                                                                                                                                                                                                   <  1:176083/251‑1 (MQ=255)
    gTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgatccg                                                                                                                                                                                                                                >  1:86017/1‑250 (MQ=255)
                                                 aCTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAATGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGcct                                                                                                                                                                                  >  1:247827/1‑249 (MQ=255)
                                                   tCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGAATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAATGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTAACCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTgacttt                                                                                                                                                                                >  1:223693/1‑246 (MQ=255)
                                                                                       aCGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGtt                                                                                                                                            <  1:273033/251‑1 (MQ=255)
                                                                                              gATGGCGGAATTTTTCCTGTTAGTCGGACTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTCTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACAATGCCGATCCGATTACCCGCGAAGGGTGGGGGCTCCCGGCGGCTACTGACATGGCCTTTGCACTTGGTGTGTTGGCGCTGTTGGGAAGTCGGGTTCCGtt                                                                                                                                       >  1:69290/1‑249 (MQ=255)
                                                                                                     gTATTTTTCCTGTTAGTCGGTCTGGAAGTTCAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCCTTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGa                                                                                                                               >  1:125816/1‑250 (MQ=255)
                                                                                                                gTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGACCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGGTCCGTTAGCGCTGAAGATCTTTTTGa                                                                                                                   <  1:286020/251‑1 (MQ=255)
                                                                                                                   aGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGTAGCCGTGTTGCGTTATCGCTGAAGATCTTTTTGATgg                                                                                                                >  2:258969/1‑251 (MQ=255)
                                                                                                                               aGTTAAACTGGAACCGAGGCAAGTAGCGCTAGCCAGCGTGCGCCAGGCCGCATTGCCAGTTATCGCCGCTATTGGTGGGATGAGTGTGCCGGCATTACTCTATCTGGCTTTTAACTAGGCCGAACCGATTACCCGCGAAGGTTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGTACGTCGTGTTCCGTGAGCGCGGAAGATCATTTTGATGGCTCTGGCtagta                                                                                                    <  2:125816/251‑4 (MQ=255)
                                                                                                                                         gaccttaTGCAAGGATCGCTAGCCAGCTTACTTGTGGTCGCATTTCCAGTTATCGCCGCCATTGGTGGGCTTATTGTTCCGGCATTACTCTATCTGGCCCTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCTGCTACTGACATTGCCTTTGCACTTTGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCTGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATc                                                                                           <  2:86017/244‑1 (MQ=255)
                                                                                                                                               aTGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGc                                                                                    <  1:30785/251‑1 (MQ=255)
                                                                                                                                                 gCAAGGATCGCTAGCCAGCTTACGCCCGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGGTAGCGCTGAAGATCTTTTTGCTGGCTCTGGCTATTATCGGCGATCTTGGGGcac                                                                                  >  2:67205/1‑249 (MQ=255)
                                                                                                                                                   aaGGATCGCTAGCCAGCTTACGCGAGGCCGCATTTCCAGATATCGCCGCTGTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGAGTTGGCGATCCCGGCAGCTCCTGACATTGCCTTTGAACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCCATGAAGATCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGccgtc                                                                                >  1:139831/1‑248 (MQ=255)
                                                                                                                                                     ggATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGTCGATCTTGAGGCcatcat                                                                              >  1:52636/1‑251 (MQ=255)
                                                                                                                                                          gCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGCGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGATACTGCCATTGCCTTTGCACTTGGAGTGCGGGCGCGGTTTGGAAGTCGTGTTCCGTTAGCGCTGAGGATCTTTTTTATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTatc                                                                          >  2:203872/1‑250 (MQ=255)
                                                                                                                                                                             gCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTATTCTACACTa                                                       >  1:17402/1‑250 (MQ=255)
                                                                                                                                                                                       cAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCg                                            <  1:67205/251‑1 (MQ=255)
                                                                                                                                                                                           tATCGCCGCTATTGGTGGGACGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATg                                         <  1:108404/250‑1 (MQ=255)
                                                                                                                                                                                            aTCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATgg                                        <  1:313442/250‑1 (MQ=255)
                                                                                                                                                                                                     aTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGAGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGATGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTgg                              >  1:343046/1‑251 (MQ=255)
                                                                                                                                                                                                       tggtggGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCATTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTCTTATCGTAGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTATCTTGGCg                            >  1:44450/1‑251 (MQ=255)
                                                                                                                                                                                                                gatTGTTCCGGAATTAATCTATCTGGCCTTTAACTATGCCGATCCGCTTACCAGCGCAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGAAGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACAATAATGACTTATCGATGTCCTCTCTTGGCGTCGCGGCtt                   >  2:231511/1‑250 (MQ=255)
                                                                                                                                                                                                                              taccttATCTGGCTTTTAACTCTGCAGATCCGATTACCCGCGTAGGGTTGGCGATCCCGGCGGCTACTGTCATTGCTGTTGCTCTTGGTGTACTGGCGCTGTTGGGAAGTTGTGTTCCGTTAGCGCTGAAGATCTTTTTTATGGCTCTGTCTCTTATCGACGATCTTGGGGCCATCATTCTCATCGCATTGTTCTACCCTAATGCCGTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGt      <  2:255254/245‑1 (MQ=255)
                                                                                                                                                                                                                               aCTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAc    >  1:239124/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tctATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTc  >  1:311070/1‑251 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
GATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTC  >  NC_000913/17595‑18070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: