Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,809 G→T V107V (GTG→GTT nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,8090GT84.0% 49.5 / ‑1.6 25V107V (GTG→GTTnhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (13/8);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTG  >  NC_000913/17565‑18053
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
tgatTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGcc                                                                                                                                                                                                                                                >  1:255254/1‑250 (MQ=255)
ggatTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCa                                                                                                                                                                                                                                                <  1:365464/250‑1 (MQ=255)
                              gATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgat                                                                                                                                                                                                                  <  1:176083/251‑1 (MQ=255)
                                  gTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgatccg                                                                                                                                                                                                               >  1:86017/1‑250 (MQ=255)
                                                                               aCTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAATGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGcct                                                                                                                                                                 >  1:247827/1‑249 (MQ=255)
                                                                                 tCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGAATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAAATGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTAACCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTgacttt                                                                                                                                                               >  1:223693/1‑246 (MQ=255)
                                                                                                                     aCGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGtt                                                                                                                           <  1:273033/251‑1 (MQ=255)
                                                                                                                            gATGGCGGAATTTTTCCTGTTAGTCGGACTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTCTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACAATGCCGATCCGATTACCCGCGAAGGGTGGGGGCTCCCGGCGGCTACTGACATGGCCTTTGCACTTGGTGTGTTGGCGCTGTTGGGAAGTCGGGTTCCGtt                                                                                                                      >  1:69290/1‑249 (MQ=255)
                                                                                                                                   gTATTTTTCCTGTTAGTCGGTCTGGAAGTTCAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCCTTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGa                                                                                                              >  1:125816/1‑250 (MQ=255)
                                                                                                                                              gTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGACCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGGTCCGTTAGCGCTGAAGATCTTTTTGa                                                                                                  <  1:286020/251‑1 (MQ=255)
                                                                                                                                                 aGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGTAGCCGTGTTGCGTTATCGCTGAAGATCTTTTTGATgg                                                                                               >  2:258969/1‑251 (MQ=255)
                                                                                                                                                             aGTTAAACTGGAACCGAGGCAAGTAGCGCTAGCCAGCGTGCGCCAGGCCGCATTGCCAGTTATCGCCGCTATTGGTGGGATGAGTGTGCCGGCATTACTCTATCTGGCTTTTAACTAGGCCGAACCGATTACCCGCGAAGGTTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGTACGTCGTGTTCCGTGAGCGCGGAAGATCATTTTGATGGCTCTGGCtagta                                                                                   <  2:125816/251‑4 (MQ=255)
                                                                                                                                                                       gaccttaTGCAAGGATCGCTAGCCAGCTTACTTGTGGTCGCATTTCCAGTTATCGCCGCCATTGGTGGGCTTATTGTTCCGGCATTACTCTATCTGGCCCTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCTGCTACTGACATTGCCTTTGCACTTTGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCTGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATc                                                                          <  2:86017/244‑1 (MQ=255)
                                                                                                                                                                             aTGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGc                                                                   <  1:30785/251‑1 (MQ=255)
                                                                                                                                                                               gCAAGGATCGCTAGCCAGCTTACGCCCGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGGTAGCGCTGAAGATCTTTTTGCTGGCTCTGGCTATTATCGGCGATCTTGGGGcac                                                                 >  2:67205/1‑249 (MQ=255)
                                                                                                                                                                                 aaGGATCGCTAGCCAGCTTACGCGAGGCCGCATTTCCAGATATCGCCGCTGTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGAGTTGGCGATCCCGGCAGCTCCTGACATTGCCTTTGAACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCCATGAAGATCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGccgtc                                                               >  1:139831/1‑248 (MQ=255)
                                                                                                                                                                                   ggATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGTCGATCTTGAGGCcatcat                                                             >  1:52636/1‑251 (MQ=255)
                                                                                                                                                                                        gCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGCGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGATACTGCCATTGCCTTTGCACTTGGAGTGCGGGCGCGGTTTGGAAGTCGTGTTCCGTTAGCGCTGAGGATCTTTTTTATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTatc                                                         >  2:203872/1‑250 (MQ=255)
                                                                                                                                                                                                           gCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTATTCTACACTa                                      >  1:17402/1‑250 (MQ=255)
                                                                                                                                                                                                                     cAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCg                           <  1:67205/251‑1 (MQ=255)
                                                                                                                                                                                                                         tATCGCCGCTATTGGTGGGACGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATg                        <  1:108404/250‑1 (MQ=255)
                                                                                                                                                                                                                          aTCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATgg                       <  1:313442/250‑1 (MQ=255)
                                                                                                                                                                                                                                   aTTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGAGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGATGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTgg             >  1:343046/1‑251 (MQ=255)
                                                                                                                                                                                                                                     tggtggGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCATTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTCTTATCGTAGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTATCTTGGCg           >  1:44450/1‑251 (MQ=255)
                                                                                                                                                                                                                                              gatTGTTCCGGAATTAATCTATCTGGCCTTTAACTATGCCGATCCGCTTACCAGCGCAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGAAGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACAATAATGACTTATCGATGTCCTCTCTTGGCGTCGCGGCtt  >  2:231511/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
TGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTG  >  NC_000913/17565‑18053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: