Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,436	C→T	intergenic (+426/‑53)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,436	0	C	T	100.0%	39.8 / NA	14	intergenic (+426/‑53)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/3);  total (11/3)

GGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGT  >  NC_000913/17199‑17669                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          ggATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTaaa                                                                                                                                                                                                                               <  1:171278/251‑1 (MQ=255)                      actaacaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCAACTTAAGCTATGATTATCTGGGCTTAGGGTCACTCTTGAGCGCTTACAGCCGTCAACAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGAATTGAGCGGGTTAAAGTAGTAAATACGATCTATTcc                                                                                                                                                                                                          >  2:273033/13‑250 (MQ=255)                          acaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTg                                                                                                                                                                                                      >  1:327374/9‑251 (MQ=255)                               taacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa                                                                                                                                                                                                    >  1:311219/4‑248 (MQ=255)                                                             cTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGAtt                                                                                                                                                                   >  1:220286/1‑251 (MQ=255)                                                                                      tCCTCAACTTAAGCTATGATTATATGGAATTAGGGTCACTCGTGAGCGCTTACAGCAGTCAACAAA‑CTAATCACACCGCTGATGGCGCAAATTATACAATGGATAGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAACCGATCCATTCTCCTGAATGCGAAATAAACAGTGAAACATCTTCATCGCTTCTCTAGCAGttttgcctagagaggc                                                                                                                                          >  2:176083/1‑236 (MQ=255)                                                                                                aaGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCtt                                                                                                                                 >  1:66984/1‑250 (MQ=255)                                                                                                                 gCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATATGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTg                                                                                                               >  1:35340/1‑251 (MQ=255)                                                                                                                             tCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATg                                                                                                   >  1:204276/1‑251 (MQ=255)                                                                                                                                         aCAGCCGTGAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCCATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGAGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGCAATAAAAAGAGAAACATATGCATCGATTCTTTAGAAGTGATGCCTCGGGCGGCATTATTCTATTCATTGCCGCTATCCTGGCGATGATTATGGCCaatcagggc                                                                                       >  2:192929/1‑244 (MQ=255)                                                                                                                                                           cATCTCCCCGCTGATGGCGCAAATTCTTCAATGGATTGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCTCCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTATCAGTTATTCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTTGCGATTATTATGGCCAATAGCGGCGTAACCATTGGATGGTa                                                                      <  2:220286/251‑1 (MQ=255)                                                                                                                                                                          ggCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACCCTATAATCTGATTTTAACGATGATTTGTTCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGCTGATTATGGCCAATAGCGACGCAACCAGTGGATGGTATCACGTCTTTCTgg                                                        >  1:208271/1‑250 (MQ=255)                                                                                                                                                                                                 tAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGggttg                                >  1:247118/1‑251 (MQ=255)                                                                                                                                                                                                                               ggagtttAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAATTGAAACATCTGTATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCACTGCCGCTATCCTGGCGATGATCATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTGCGCTCGAAATCAACAAAAACATGCTGt  <  1:293815/247‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGT  >  NC_000913/17199‑17669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑