Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,399	A→G	intergenic (+389/‑90)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,399	0	A	G	100.0%	39.5 / NA	14	intergenic (+389/‑90)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/2);  total (12/2)

TAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTG  >  NC_000913/17156‑17639                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tAACTTTAATTTCTCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTc                                                                                                                                                                                                                                            >  1:290854/1‑251 (MQ=255)                                            ggATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTaaa                                                                                                                                                                                                 <  1:171278/251‑1 (MQ=255)                                                                 actaacaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCAACTTAAGCTATGATTATCTGGGCTTAGGGTCACTCTTGAGCGCTTACAGCCGTCAACAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGAATTGAGCGGGTTAAAGTAGTAAATACGATCTATTcc                                                                                                                                                                            >  2:273033/13‑250 (MQ=255)                                                                     acaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTg                                                                                                                                                                        >  1:327374/9‑251 (MQ=255)                                                                          taacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa                                                                                                                                                                      >  1:311219/4‑248 (MQ=255)                                                                                                        cTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGAtt                                                                                                                                     >  1:220286/1‑251 (MQ=255)                                                                                                                                 tCCTCAACTTAAGCTATGATTATATGGAATTAGGGTCACTCGTGAGCGCTTACAGCAGTCAACAAA‑CTAATCACACCGCTGATGGCGCAAATTATACAATGGATAGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAACCGATCCATTCTCCTGAATGCGAAATAAACAGTGAAACATCTTCATCGCTTCTCTAGCAGttttgcctagagaggc                                                                                                            >  2:176083/1‑236 (MQ=255)                                                                                                                                           aaGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCtt                                                                                                   >  1:66984/1‑250 (MQ=255)                                                                                                                                                            gCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATATGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTg                                                                                 >  1:35340/1‑251 (MQ=255)                                                                                                                                                                        tCGTGAGCGCTTACAGCCGTCAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATg                                                                     >  1:204276/1‑251 (MQ=255)                                                                                                                                                                                    aCAGCCGTGAA‑AAAACTCATCTCACCGCTGATGGCGCAAATTCTTCCATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGAGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGCAATAAAAAGAGAAACATATGCATCGATTCTTTAGAAGTGATGCCTCGGGCGGCATTATTCTATTCATTGCCGCTATCCTGGCGATGATTATGGCCaatcagggc                                                         >  2:192929/1‑244 (MQ=255)                                                                                                                                                                                                      cATCTCCCCGCTGATGGCGCAAATTCTTCAATGGATTGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCTCCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTATCAGTTATTCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTTGCGATTATTATGGCCAATAGCGGCGTAACCATTGGATGGTa                                        <  2:220286/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACCCTATAATCTGATTTTAACGATGATTTGTTCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGCTGATTATGGCCAATAGCGACGCAACCAGTGGATGGTATCACGTCTTTCTgg                          >  1:208271/1‑250 (MQ=255)                                                                                                                                                                                                                                            tAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGggttg  >  1:247118/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAA‑AAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTG  >  NC_000913/17156‑17639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑