Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,593 A→G A419A (GCA→GCG)  thrA → fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,5930AG93.5% 67.6 / ‑2.8 31A419A (GCA→GCG) thrAfused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (13/16);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

CACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGT  >  NC_000913/1355‑1835
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
cACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCATCCGAATACAGTATCAGTTTCTGAGTTCCGAAAAGCGCCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGAAGGCCATTATCACGGGGGTAGGGGATGGTATGCGCACCTTGAGGGGGCACCCGGCGATATTCTTTGCCGAGCTGGcccaggcc                                                                                                                                                                                                                                        >  1:199687/1‑246 (MQ=255)
                         gATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTc                                                                                                                                                                                                               <  1:217274/251‑1 (MQ=255)
                          aTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTc                                                                                                                                                                                                               >  1:338677/1‑250 (MQ=255)
                                     atcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAAAGGATGGCCATTATCTCGGGGGTAGGTGGGGGTATGCGCAACTTGCGTGGGGACTCGGCGGTAATGTTTGCCGCGCTGGCCCGCCCCAATATCAACATTGTCGCCCTTGAGCACGGAtcttc                                                                                                                                                                                                     >  2:326043/1‑249 (MQ=255)
                                      tcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTg                                                                                                                                                                                                   >  1:165951/1‑250 (MQ=255)
                                         tCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGA‑GGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAAc                                                                                                                                                                                                <  1:124831/249‑1 (MQ=255)
                                                     aTCAGTGTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCTATTGCTCAGGGATCTGCTGAACGCTCAATCTCTGt                                                                                                                                                                                   <  1:303156/251‑1 (MQ=255)
                                                               gctgtccggaaaGCGACGGTGAGCGGGCTGAACGGGGAATGCAGGGAGAGGTCTCGCTGGAACTGAAGGAAGGCTGACTGGAGGCGCGGGTGGGGACGGAACGGTGGGCTATTATCTCGGTGGGAGGTGATTGGAGGCGCACCTGGCGTGGGATCTCGGCGAAAGTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCGGAACGCTCAATGTCTGTCTTGGTAAAt                                                                                                                                                                         <  2:161515/242‑1 (MQ=255)
                                                                         aaGCGACTGGCTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCCCCTTGCGTGGGATCTCGGCTAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGGTCAGGGAGCTTCTGAACGCTCAGTCTCTGTCGTGGTAAATAACGatgatg                                                                                                                                                               <  1:144828/251‑1 (MQ=255)
                                                                           gCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCg                                                                                                                                                             >  1:253960/1‑251 (MQ=255)
                                                                            cgcatgtgtgctatgttaactgggaaTGCAGGAAGAGTTCTTCCTGGACCTGAAAGAAGGCTTGCTGGAGCCTCTGGCGGTGACGGACCGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGa                                                                                                                                                            <  2:11471/227‑1 (MQ=255)
                                                                                          cTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGGTAAGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGGTCTTCTGAACGCTCAATCTCTGTCGTGGt                                                                                                                                                                             >  1:335418/1‑220 (MQ=255)
                                                                                                     aTGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCTCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca                                                                                                                                   <  1:103861/251‑1 (MQ=255)
                                                                                                                          gAACTGAAAGAAGGCTTACTGGAGCAGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGGATGAGCACCTTGCGTGGGGTCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCCCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGAGCGCGTTACTCATCCGATGCTGTTTAATACCGaac                                                                                                               >  1:306410/1‑248 (MQ=255)
                                                                                                                             cTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTTGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAAAATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGGTGAAGCGACCAATGGCGTGCGCGTTACTCATCAGATGCTGTTCAATCCCGATCAggg                                                                                                           >  1:302188/1‑250 (MQ=255)
                                                                                                                                aaaGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTAt                                                                                                        <  1:67663/251‑1 (MQ=255)
                                                                                                                                                      tGGCGGTGACGGAACGGCTGGCCATTATTTTGGTGGGAGGTGATGGTATGCGCACCCTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCAACAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTc                                                                                  >  1:123168/1‑251 (MQ=255)
                                                                                                                                                       ggcggTGACGGAATGGTTGGCCATTATCTCGGTGGGAGGTGATGGGATGCGCGCCGTGCGGTGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCg                                                                                 <  2:288428/247‑1 (MQ=255)
                                                                                                                                                                     ggCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTGTGCCTCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACga                                                                                                                                                                    <  1:246443/154‑1 (MQ=255)
                                                                                                                                                                         ggCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTgcgc                                                               >  1:371715/1‑251 (MQ=255)
                                                                                                                                                                               tATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAgg                                                                                                            <  1:42521/200‑1 (MQ=255)
                                                                                                                                                                                  ctcGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGc                                                      >  1:246174/1‑251 (MQ=255)
                                                                                                                                                                                     ggtggtAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAAc                                                   <  1:326043/251‑1 (MQ=255)
                                                                                                                                                                                        ggtAGGTGATGGTTTGCGCTCCTTGCGTGGGACTTAGTCGAATGTCTTTTCCGCGCTTGACCGCGACAATATCATCATTGTCGTCATTGCTCAGGGATCTTCTGAACTCTCAATCTCTGTCGTGGTAAAGAACGATGATGCGACCACTGGCGTGCGCGTTACGCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCTTCTGTGGCGTTGGCGGTGCGCTGCTGGAGCAGCTGa                                                <  2:292225/251‑1 (MQ=255)
                                                                                                                                                                                           aGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTCGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTGGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAg                                              <  1:384515/250‑1 (MQ=255)
                                                                                                                                                                                               gATGGTATGCGCACCTGGCGTGGGATCGCGTCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATATCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCa                                         <  2:253960/251‑1 (MQ=255)
                                                                                                                                                                                                       gcgcACCTTGCTTGGGATCTTGGCGAAAGTCTTTGCCGCGCTGGCCCTCGCCCATATCAACATTGGCGCCATTTCTCAGGGATCTTCTGAACGCGCAATCTCTTTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTGCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACaaatc                                  <  2:339509/250‑3 (MQ=255)
                                                                                                                                                                                                                  gTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTAATCATCAGATGCTGTTCAATACCGATCAGGATATCGAGGTGTTTGTGATTGGCGTCGGGGGCGTTGGCGGTGCGCTGCTGGGGCAAATGAAGCGTCAACAAAGCGGGCTgaaga                        >  2:137301/1‑249 (MQ=255)
                                                                                                                                                                                                                           cGGCGAAATTCTTTCCCGCGCTGGCCCGCGCCACTATCAACCTTGACGCCATTGCTCAGGGCTCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGAACACTGGCGTGCGCGTTCCTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGCAGTGTTTGTGCTTGGCGTCGGTGGAGTTGTCGGTGCGCTGCTGGAGCAACTGAACCGTCAACCAAGCTGGCTGATGAATAAACCTATc             >  2:171287/1‑251 (MQ=255)
                                                                                                                                                                                                                               gAAATTCCTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACt         <  1:71942/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ttGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACgtgt  <  1:250346/250‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
CACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGT  >  NC_000913/1355‑1835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑