Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 15,338 A→T intergenic (+40/‑107) dnaJ → / → insL1 chaperone protein DnaJ/IS186/IS421 family transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091315,3380AT88.0% 55.4 / 1.6 25intergenic (+40/‑107)dnaJ/insL1chaperone protein DnaJ/IS186/IS421 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base T (7/15);  total (7/18)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

GGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTG  >  NC_000913/15092‑15582
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
ggcggcaggTCTGTCCGGGGTGGCGCTCAGGGTGATTTGCTGTGCCGGGGTGTCGTCGAAACTCCGGTAGGCCCGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGAAGACCTGACCCGCTAACCTCCCTAAAAGCCGGCCCGCGGGCAGGCCTGGGTAAAAATAgg                                                                                                                                                                                                                                                  <  2:217816/243‑1 (MQ=255)
      aaGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGCGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAAAAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGAAAAATTAGGGTgagt                                                                                                                                                                                                                                            >  1:226369/1‑248 (MQ=255)
          cTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGa                                                                                                                                                                                                                                         >  1:314710/1‑250 (MQ=255)
                    gggCGCACGGGGTGATTTGGTAGGACGCGTTGTCGACAAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGTCCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTTCGTTGAAGATATGCGAg                                                                                                                                                                                                                              <  2:166403/250‑1 (MQ=255)
                       cgcACAGGGTGATTTGCTGTTCCGCGTTGTCGTCGAAACACCGCTTGGCCCTAACGAGAAGCAGAAACAGCTGCTTCAAGCGCTGCAAGAAAGCTTCGGTGGCCCAATCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGACGGGGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGTGCAGGCTTGGGTAAAATTAGGGTGCATTGAAGATATGCGAGCAc                                                                                                                                                                                                                           <  2:126426/251‑1 (MQ=255)
                          acaGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTg                                                                                                                                                                                                                        <  1:32788/251‑1 (MQ=255)
                                           gcctcgTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACTCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCa                                                                                                                                                                                                       <  1:141880/247‑1 (MQ=255)
                                             cgagTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGCAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCTTGAGCAGGCTTGGGAAAAATTAGGGCACGTTGAAGATATTCGAGCACATGAAAAGGGGCggggtgacac                                                                                                                                                                                                     >  2:21931/4‑245 (MQ=255)
                                                     cgtcgAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCgggggagacacccctaa                                                                                                                                                                                              >  1:382962/1‑237 (MQ=255)
                                                     cgtcgAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCCGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCTCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGTGATCACTCCCATAAg                                                                                                                                                                                             <  1:190544/251‑1 (MQ=255)
                                                              acCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCgggggacaatcccatcaacgcaaactt                                                                                                                                                                                    >  1:351540/1‑228 (MQ=255)
                                                              acCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACtt                                                                                                                                                                                    <  1:315028/251‑1 (MQ=255)
                                                                cGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCAAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGaatacacccagacaaacgacatta                                                                                                                                                                                   >  1:202854/1‑227 (MQ=255)
                                                                  gTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCACGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGACGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGATGCGTTGAAGATATGCGAGCAACTGAAAAGTGGCgggggatactactacaagagcaacaaga                                                                                                                                                                                   >  1:335145/1‑224 (MQ=255)
                                                                               agaagCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAc                                                                                                                                                                   <  1:316109/247‑1 (MQ=255)
                                                                                     aGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                                                                                                             <  1:148710/251‑1 (MQ=255)
                                                                                            gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAtt                                                                                                                                                      <  1:350922/251‑1 (MQ=255)
                                                                                               gctgcaagagctgcaagaAAGCTTCGGTGGCCCACCCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTaa                                                                                                                                                   <  1:69142/251‑1 (MQ=255)
                                                                                                                          gggCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTTGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTACCGATGAATTACTCTCACGATaa                                                                                                                        <  1:121477/250‑1 (MQ=255)
                                                                                                                                          cacaACAGCCTGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCTGGGGTAAAATTAGGGTGCGTTGAAGATATGCGAGCTCCTGAAAAGTGGCGGGGATCACTCCCATAATCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCTCGATAACTGGTCAGCAATTCTg                                                                                                        <  2:314710/251‑1 (MQ=37)
                                                                                                                                                               agCTACTTTGATAGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaa                                                                                   <  1:81463/251‑1 (MQ=255)
                                                                                                                                                                                                                                       caggcggggttAAAATTAGGGTCCCTTGAATATATGCGAGCGCCTGAAAATGGGCGGGTATCTCTCCCTTACTCGTTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTTCTCTCCCGATAACTGGTCAGCACTTCTGGCCCATATTGGTAAGCCCGAAGAACGGGATGCTTCTGCACGTATTGCCGGGGTTCGAACCCGCCGCCGCGAAATTCGTGATGCGGCAACCCTg           <  2:169797/241‑1 (MQ=2)
                                                                                                                                                                                                                                             ggggTAAAATTAGGGTTCGTTGATGATGTGCGAGTATCGGAAAAGTGGCTGGGTTCACGCCCAAAAGCGTTAACTTAAGGGTTGTGGTATTACGCATGATTTGATTTAACGTGCCGATGAATTACTCTAACGCTAACTGGTCAGCAATTCTGGCCCATATTTGTAAGCCCGAAGAACTGGATACATCGGCACGTAATGCTGGGGCTATAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTAc       <  2:294729/248‑1 (MQ=11)
                                                                                                                                                                                                                                               ggTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCt   <  1:202085/251‑1 (MQ=255)
                                                                                                                                                                                                                                                gTAAAATTAGGGTGCGTTGAAGATATGCGAGCACCTGAAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTg  <  1:344191/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
GGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTG  >  NC_000913/15092‑15582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑