Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,073	A→T	L302L (CTA→CTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,073	0	A	T	92.9%	117.3 / ‑0.8	43	L302L (CTA→CTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (15/24);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

ACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCC  >  NC_000913/14838‑15312                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   aCCGCATCCGGCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGt                                                                                                                                                                                                                                     <  1:29701/251‑1 (MQ=255)  ccGCAGCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGAATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTa                                                                                                                                                                                                                                    <  1:373753/251‑1 (MQ=255)       cccGTCTGGCGGGCGAAGGTGAAGTGGGCGAGCATGGCGACCCGGGAGGCGAGCGGTACGGTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCTACAACCTCGATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGTCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGTTAAGCTTTTCCGTATGCGCGGTAAAGGc                                                                                                                                                                                                                               <  2:336343/250‑1 (MQ=255)          gtcgtGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGCGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGAGAAACCCAGAACAGAAAGCTTTTCCGCATGCGCGGGAAAGGCGTc                                                                                                                                                                                                                            >  1:182755/5‑251 (MQ=255)              ggCGGGCTAGGGTGAAGTGGGCGAGCAGGGCGTACCGGAAGGCGATCTGGTGGTTCAGTTGCATGTTAAACAGCACCCGATTGTCGAGCGTGAAGTCAACTACCTCTATTTCGAAGGCGCGATCAATTTCGTAATGGCGGCGCTGTGTGTCGAAATCGAAGTGCCTACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAATCCCTGACCGGTAATCTTTTCGGTATGCTCCGTAAATGCGTCAAg                                                                                                                                                                                                                         <  2:339477/249‑1 (MQ=255)                           gAAGCGGGCGAGCAGGACGCATCGGCAGGCGATCTGTACGTTCAGGTCCAGGTTTAACTGCTCCCGATTCTCGAGCGTGACGGCAACAACCTCCATTGCGATGTCCCCATCAACTTCGCTATGGCGGCGCTTGGTGGTGAAATCGAAGTACCTACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTTTTTCCGCGGTgg                                                                                                                                                                                                           <  2:95927/251‑1 (MQ=255)                             aGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGgcg                                                                                                                                                                                                         >  1:217816/1‑251 (MQ=255)                                        aTGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGAt                                                                                                                                                                                              >  1:294729/1‑251 (MQ=255)                                                              gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTgtcg                                                                                                                                                                          <  1:167240/248‑1 (MQ=255)                                                              gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGCCCGGTAAACTTTTCCGGATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGtcgtcg                                                                                                                                                                       >  2:148881/1‑251 (MQ=255)                                                              gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTgtcgtc                                                                                                                                                                        <  1:151267/250‑1 (MQ=255)                                                                 cGTTCAGGTTCAGGTTAAACAGCCCCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGAGAAGGCCCGATCAACTTCGCTATGGCGGCGATGGGTGGAGAAATCGAAGGACCGACACTTGATGGTAGCGTCAAACTGAAAGAGCCTGGCGAAACCCAGCCCGGTAAGATTTTCCGGATGCGCGGTAAAGGCGTCCAATCTGTCCGCGGGGGAGCACAGGGTGTG‑TTGTTGTGCcgcggttgagggtaa                                                                                                                                                                     >  2:221192/1‑239 (MQ=255)                                                                          tcaggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGACGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGt                                                                                                                                                             >  1:130468/1‑249 (MQ=255)                                                                            aggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGc                                                                                                                                                         <  1:141862/251‑1 (MQ=255)                                                                              gttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGGAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTTTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGAGCCGCGTTGTCGTCGAAACACCGGTAGGCCt                                                                                                                                                       >  1:329363/1‑251 (MQ=255)                                                                                  aaCAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAAc                                                                                                                                                   >  1:326848/1‑251 (MQ=255)                                                                                    cAGCACCCGATTGTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACGTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTTCCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAG‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑a                                                                                                                                                <  1:41382/251‑1 (MQ=255)                                                                                     cgaaaccgatgtccgggCGTGGAGGCTACAACCTCGATTGCGAAGTCCCGAGCAACTACGATATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGGCAAGTCTGTCCGTGGTGGCGCACAGGGTGAT‑TTGATGTGCAGCGTTGTCGTCGAAACTCCGGTAGGCCTGGACG‑ag                                                                                                                                               <  2:82556/235‑2 (MQ=255)                                                                                                   gAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAgct                                                                                                                                 <  1:162003/251‑1 (MQ=255)                                                                                                         gatggCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGAGCTGGGGGGGGGAATCGAAGTACCGTCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAAACCGGATAGCTATTCCGGATGGGCAGTAAAAGAGGCCAGTCTGGCCGCGGGGGGGCACCGGG‑GAGGTTGCTGGGCCGCGTTGTCGTCTAAACACCGGTAGGCCGGTACG‑AAAgggaggaaaaagtgcggc                                                                                                                            >  2:190544/4‑234 (MQ=255)                                                                                                               aacaacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTgctgcaagagct                                                                                                                     <  1:148881/251‑1 (MQ=255)                                                                                                               aacaacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAAGCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTgctgcaagagct                                                                                                                     <  1:95063/251‑1 (MQ=255)                                                                                                                                aGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTg                                                                                                    <  1:236094/251‑1 (MQ=255)                                                                                                                                                     ggcggcGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCacaaca                                                                               <  1:229422/251‑1 (MQ=255)                                                                                                                                                        ggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCt                                                                                                                                                       >  1:161194/1‑177 (MQ=255)                                                                                                                                                        ggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCGGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCt                                                                                                                                                       <  2:161194/177‑1 (MQ=255)                                                                                                                                                           gcTGGGTGGCGAAATCGAAGTACCGACCCATGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTACCGTATGCGCGGAAAAGGCGTCAAGTCGGTCCGCGGTGGAGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTAGAAACACCGGTGAGCCTGAACGTGCAGAC‑GCAACAGCGGCTGCAAATACTGCAAGAACGCTTCGGTGGCCCAACCgtagagcaaaaccgccagc                                                                         >  2:362496/1‑233 (MQ=255)                                                                                                                                                                gTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCa                                                                    <  1:321470/251‑1 (MQ=255)                                                                                                                                                                gTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCa                                                                    <  1:182578/251‑1 (MQ=255)                                                                                                                                                                       aaTCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGct                                                            <  1:315207/251‑1 (MQ=255)                                                                                                                                                                          cGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGGCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGcttc                                                          <  1:221192/250‑1 (MQ=255)                                                                                                                                                                          cGAAGTACCGAACCTTGATGGTCGCGGCAAACTGACAGTGCCTGGCGAAACCCAGACCGGTACGCTTTTCCGTATGCGCGGTACAGGCGTCAAGTCTGTCCGCGGTGCCCCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGCAGCGGAAACCGCTGCTGCAAGAGCCGCACGAAAGCTTCGGGGGCCCAACCGGAGAGCACCACACCCCGCGCTCC‑Aagacatact                                                         >  2:350922/1‑245 (MQ=255)                                                                                                                                                                               tACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTgctgca                                                                                                                           >  1:372293/1‑181 (MQ=255)                                                                                                                                                                                aCCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGAt                                                    <  1:239708/250‑1 (MQ=255)                                                                                                                                                                                 ccggcgcTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTACGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGTCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGATGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCAAAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATgg                                                  <  1:98661/245‑1 (MQ=255)                                                                                                                                                                                       cTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCAGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGgtgaga                                             >  1:239972/1‑247 (MQ=255)                                                                                                                                                                                               tCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑GGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGTTCA‑AAAAACTTATTTGATGGGGGgaagaacgatc                                    >  2:91296/1‑246 (MQ=255)                                                                                                                                                                                               tCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTgaagaa                                         <  1:91296/246‑1 (MQ=255)                                                                                                                                                                                                cgcttCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCCCAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAACCAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGGGGCCCAACCGGTGAGCACAAACGCCCGCGCTCA‑CAGAGCTTCTTTGATGGTGTGGATAAGttttt                                   >  2:121477/5‑251 (MQ=255)                                                                                                                                                                                                cgcgTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTGAAGAAGtttt                                    <  1:251252/250‑1 (MQ=255)                                                                                                                                                                                                         cTGAAAGTGCCTGGAGAAACCCAGACCGGTAAGCTTTTCCGGATGCGCGGCAAAGGCGCCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TGGCAGGGCCGCGTTGACGTCGGAACACCGGTCGACGTGAACG‑AGAACAAGAGAGAGCTGCTGCAAGAGATGCAAGAACGCTTCGGGGGGCCAACAGGAGAGCAAACCCGCACGCGC‑CACAAGACCATCTTTGATGGGGAGAAGACGATTTTTGACGACCt                          >  2:239963/1‑251 (MQ=255)                                                                                                                                                                                                                     ggCGAAACCCAGACCGGTAAGCTGTTCCGTATGCGCGGTAAAGGCGGCAAGTCTTACCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGGCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAAcc              <  1:259046/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGcc  <  1:218698/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT‑TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACG‑AAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCA‑AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCC  >  NC_000913/14838‑15312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑