Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,232	G→A	R22K (AGA→AAA)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,232	0	G	A	91.3%	56.6 / ‑2.6	24	R22K (AGA→AAA)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (16/5);  total (18/6)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

TTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGC  >  NC_000913/14010‑14476                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      gtctgaacacaacGAGAAAGATGGCGATGGTGTCGACGCAGAATTTGAAGAAGGCAAAGACAAAAAATAATTGCCCGATAAACGGTTAATTATTCTGAAACTGGCGAAGGGGAATTTCCTCGCCGCCCGAGCAGTCATCTAGGGGCAATGTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGaaa                                                                                                                                                                                                                           <  2:128681/243‑1 (MQ=255)                                                                      atCGCCCTATAACAGGGGAATTATAAAGAGACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTCCAACCGCCTGGCCATGAAATACCACCCGGCCCGTAACCAGGGTGACACAGAGGCCGAGGCGAAATTTAAAGAGATCAAGTAAGCTTAt                                                                                                                                                      >  1:290528/1‑251 (MQ=255)                                                                        cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGCTTATTACGAGATTTTAGGCGTTTCAAAAACAGCGGAAGAGCATGAAATCAAAAAGGCATACAAACGCCTGGACATGAAATACCACCCGGACCGTAACCAGGGGGACAAAAAGGCCAAGGCGAAAATTAAAGAGAGCAAGGAAGCTTATg                                                                                                                                                     >  2:324289/1‑250 (MQ=255)                                                                          cccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAGTTTAAAGAGATCAAGGAAGCTTATGa                                                                                                                                                    >  1:308768/1‑249 (MQ=255)                                                                                                tgacacaagagAAGGGGAATTTCCTATCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGACGAGCATGAAATAAAAAAGGACTACAAACGCCTGGCCATGAAATACCACCCGGCAAGTAACCAGGGTGACATAGAGGCCGAGGCGAAATTTAACGAGCTCAAGGAAGCTTCTGAAGTTCTGACCGCCTCGCAACAACg                                                                                                                            >  2:185087/11‑251 (MQ=255)                                                                                                        gctaaGGGGAATTTCCTCGCCGCCCGTGCATTCAGCTAGGGGCAATTTAAAAAAGATGGCAAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                                                                    <  2:45812/248‑1 (MQ=255)                                                                                                                                    cATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTAATGCTGAGTTTgg                                                                                        >  1:206162/1‑250 (MQ=255)                                                                                                                                       tcatcaAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACTGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGGGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGc                                                                                      <  1:2747/250‑1 (MQ=255)                                                                                                                                             aGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAAGCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTg                                                                               <  1:185701/250‑1 (MQ=255)                                                                                                                                                  ccaTTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAAGACTACAAACGCATAGCCATGAAATACCAACCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAGTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGAATCGCAAAAACGTGCGGCATACGATCATGCTGGTCATGCGGCGTTTGAGCAAG‑GTGGTATgg                                                                         >  2:139663/3‑251 (MQ=255)                                                                                                                                                        aaaaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTAACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGCATGggcggcg                                                                   >  1:140002/1‑251 (MQ=255)                                                                                                                                                                      aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTCTGAAGTTCTGACCGACTCGCAAAAACGAGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTggcggc                                                     >  1:357470/1‑251 (MQ=255)                                                                                                                                                                         aaGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTggcggcggg                                                  >  1:177935/1‑250 (MQ=255)                                                                                                                                                                            attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGgcgc                                                >  1:108414/1‑250 (MQ=255)                                                                                                                                                                                        ttttAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGat                                   >  1:321790/1‑251 (MQ=255)                                                                                                                                                                                           tAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATAtt                                >  1:347539/1‑251 (MQ=255)                                                                                                                                                                                            aGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATAttt                               >  1:341772/1‑251 (MQ=255)                                                                                                                                                                                              gCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATAttttt                             <  1:47654/251‑1 (MQ=255)                                                                                                                                                                                                gTTTCCAAAACAGCGGAAGAGCATGAAATCACAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAATGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAATTCTGACCGACTCGCAAAAACGTGCGCCATGCGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTTTGGGCGGCGGCTGTTTTggcggcggagccaacttcctcggtattttttg                           >  2:28957/1‑227 (MQ=255)                                                                                                                                                                                                          cAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTc                 >  1:46451/1‑251 (MQ=255)                                                                                                                                                                                                              ggAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCg             >  1:175680/1‑251 (MQ=255)                                                                                                                                                                                                                   agcatGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGGGAAAACGAGGCCGAGGAGAAATTTAAAGAGATCAAGGAAGCTTAAGAAGTTCTGACCGACTCGCATAAACGTGCGGCAGACGGTCAGTCTGGTCATGCTGCGTTTGAG‑AAGTGAGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGGCTTCAGCGATATTTTTAGTGGCGTTTTAGGCGacttt        >  2:303326/5‑247 (MQ=255)                                                                                                                                                                                                                        taaaTCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGTCTCTCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGTATGGGCGGCGGCTGTTTTGTCGGCGGCGCATACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTg    <  1:283773/249‑1 (MQ=255)                                                                                                                                                                                                                         aaaTCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGCAAAAGAGACCGAGGCGCAATTTAAAGAGATCGAGGACGCTTATGAAGTCCTGACCGACTCGCAAAAACGTGCGGCATACGATAAGTATGGTCATGCTGCGTTTGCGCACG‑GTGGAATGGGCGGCGGCGGTTTTGGCGGCGGCGCCGACTTCAGCGATCTTTTTGGTGACGTTTTCGGCGATATTTTTggc  >  1:375083/1‑251 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      TTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAG‑GTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGATATTTTTGGC  >  NC_000913/14010‑14476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑