Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,932	A→G	E590E (GAA→GAG)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,932	0	A	G	81.0%	29.6 / 1.9	22	E590E (GAA→GAG)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (4/13);  total (5/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

CGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTA  >  NC_000913/13686‑14174                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTgg                                                                                                                                                                                                                                                  <  1:167294/251‑1 (MQ=255)                             gaagCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAAt                                                                                                                                                                                                                      <  1:5858/248‑1 (MQ=255)                                         aaCGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGGCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATC‑AAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAgcagca                                                                                                                                                                                                          <  1:154448/250‑1 (MQ=255)                                                     gACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCcagc                                                                                                                                                                                               >  1:45812/1‑250 (MQ=255)                                                                   agagCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGTACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTg                                                                                                                                                                                <  1:199161/251‑1 (MQ=255)                                                                                                       gctgcACAGCACCCCTAAGAAGGTTGAATAAGCATGCGACAAACTCCCGGCTGACGACAAAAATGCTATCGAGTCTGCACTGACTGCACTGGAAACGACTCTGAAAGGTGAAGACAATGCCGATATCGAAGCGAAAATGCAGGAGCTGGCACAGGATTCCCAGAAGCTGATGGAAATCGCCCATCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGaa                                                                                                                                              <  2:141439/247‑2 (MQ=255)                                                                                                               gCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTTAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑TAACGCGAAAGATGTCGATGGGGTCGAc                                                                                                                                  <  2:161681/251‑1 (MQ=255)                                                                                                                   ccGTAAGCAGGTTGAAGAAGCTGGCGACAAACTGCCGGCGGACGACAAAACTTCGATCGAGTCTGCACTGACTGCACTGGAAACGGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGATCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACGGCCTGTGCTGATGCTTCTGTA‑AA‑CAACGCGAAAGAGGACGATGTTGTCgtcgatg                                                                                                                              <  2:244576/251‑7 (MQ=255)                                                                                                                        agccgggtGAAGAGGCAGGCGACAAACTGCCGGCTGACGACAAAACTTCTATCGAGTCTGCACTGACTGCACTGGAAACGGCTCTGACAGGTCAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCTCAGGTTTCCCAGAATCTGATGTAAATCTCCCCGCAGCAACATGCCCAGCAGAAGGCTGCCGTTGCTGATGTTTCTGCA‑AA‑CAACGCGAAATATGACGATGTTTTCgtagttgaattt                                                                                                                         <  2:47017/244‑12 (MQ=255)                                                                                                                          cAGGTTGAAGAAGCAGGCGACAAACGGCCGGCGGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTga                                                                                                                       <  1:49605/251‑1 (MQ=255)                                                                                                                           aGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAGCGCGAAAGATGACGATGTTGTCGACGCTGAATTTgaa                                                                                                                      <  1:295654/251‑1 (MQ=255)                                                                                                                               gaaagaagCAGGCGACAAACTGCCAGAGTACGAACAAACAGTTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATAGAAGCGACAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGTCGCTGAATTTGaagaag                                                                                                                  <  2:32605/249‑1 (MQ=255)                                                                                                                                       cAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGAc                                                                                                          <  1:284215/251‑1 (MQ=255)                                                                                                                                              cAAACTGCCGGCTTACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTATGAAAGGTGAAGGCACAGCCGCTATCGAAGCGAAAATGCTGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTGCT‑CATAATCAA‑GCGAAAGAGGACGATGTTGTCGCCGCAGAAATGGAAGAAGTCAAAGaaaaaaca                                                                                                    >  2:2747/1‑248 (MQ=255)                                                                                                                                                aaCTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAaataa                                                                                                 <  1:362303/251‑1 (MQ=255)                                                                                                                                                aaCTGCAGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCAATGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAaata                                                                                                  >  1:333756/1‑250 (MQ=255)                                                                                                                                                       ggcggacgacAAAACTGCTATCTAGTCTGCACTTACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGccca                                                                                          <  2:4071/247‑2 (MQ=255)                                                                                                                                                           gacgacAAAACTGCTATCGAGTCTGCACATACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGGCGCTAACGAAGCGAAAATGCAGGAGGTGGCACAGGTGTCACAGAAACTGAGGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATTACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGTCCtaga                                                                                       <  2:283729/250‑3 (MQ=255)                                                                                                                                                                                             cACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAt                                                    <  1:59496/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCGAAGCGAAAATGCAGGAGCTGTCACAGGTTTCTCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CCACGCGAAAGCTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGTTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTccggtagtggccctgagtc             >  2:62049/1‑233 (MQ=255)                                                                                                                                                                                                                                             aaaTGCAGTAGCTGGCACAGGTCTCCCAGAAACTGATGGAAATCGCCCAGCAGTAACATTCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc    <  1:279905/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTa  >  1:105491/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCA‑AA‑CAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTA  >  NC_000913/13686‑14174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑