Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,714	C→T	G184G (GGC→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,714	0	C	T	84.6%	55.0 / 2.5	30	G184G (GGC→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (10/12);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

TTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAA  >  NC_000913/12476‑12954                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 atAAAGGCCAGAAATGGGCACCGCCGCAGATCTCGGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGTTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGt                                                                                                                                                                                                                                      <  1:87556/250‑1 (MQ=255)           gAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCACTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCGGGCTTACGGTCTGGACAAAGGGACCGGCAACCGTACTATCGCgg                                                                                                                                                                                                                            >  2:325194/1‑251 (MQ=255)                      cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTg                                                                                                                                                                                                                 >  1:34224/1‑251 (MQ=255)                                    gctgAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTAc                                                                                                                                                                                                      <  1:325194/248‑1 (MQ=255)                                    gctgAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACtt                                                                                                                                                                                                    <  2:73331/250‑1 (MQ=255)                                            gCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGGTTATGACCTGGGTGGTGGTACTTTCGATAttt                                                                                                                                                                                           <  1:118430/251‑1 (MQ=255)                                               gAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCta                                                                                                                                                                                        <  1:134485/251‑1 (MQ=255)                                                aaaaaaaTGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCtat                                                                                                                                                                                       >  1:166570/1‑251 (MQ=255)                                                  aaaaaTGAAGAAAACCGAGGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTTGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCtatta                                                                                                                                                                                     <  1:2078/251‑1 (MQ=255)                                                                   cttaaGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGACAGGCAACCAAAGACGAAGGCCGTAACGCTGGACTGGAAGAAAAACGTATCAGCAACGAACCGACCGCAGCTGCGCTGGCATACGGACAGGACAAAGGAACTGGAAACCGAACTATAGCGGGTTATGAACTGAGTGGAGGTACTTTCGACAATTCTATAATCGacattgcagatgtt                                                                                                                                                                    >  2:258960/4‑238 (MQ=255)                                                                       aGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATAttt                                                                                                                                                                                           >  1:191927/1‑224 (MQ=255)                                                                       aGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATAttt                                                                                                                                                                                           <  2:191927/224‑1 (MQ=255)                                                                                   tGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACAAAAGACGCAGGACGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGCACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGGGGAGGTACTTTCGATATTTCTATTTACGAAATAGACGAAGTTGCCGGGGaaaaacctt                                                                                                                                                    >  2:369725/1‑247 (MQ=255)                                                                                            aaCTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTc                                                                                                                                           >  1:57506/1‑251 (MQ=255)                                                                                                            aCCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGAAACCAAAGCCGCAGGCCCTATCGCTGGTCTGGAAGTCAAACCACTCATCCACGAACCGACCGCCGCTGCGCTGGCTTACGGTCTGGACAAAGAGACTGGCAACCGTACTATCGCGGTTTCTGACCTGggtggtg                                                                                                                                                                                                          >  1:348386/1‑172 (MQ=255)                                                                                                                         aCTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTgggg                                                                                                              >  1:59135/1‑251 (MQ=255)                                                                                                                                    gCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACtt                                                                                                   >  1:314493/1‑251 (MQ=255)                                                                                                                                      ccAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                 <  1:350608/250‑1 (MQ=255)                                                                                                                                             cAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCg                                                                                          <  1:219083/251‑1 (MQ=255)                                                                                                                                              aGGCAACCAAAGACGCAGGCCGTATCGCTGGCCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGGGCTGGATTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGGTGACGGCGAAAAAACCTTAGAAGTTCTGGCAACCAACGGGGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGt                                                                                         >  2:344375/1‑251 (MQ=255)                                                                                                                                                gCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAA‑CCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTg                                                                                      <  1:369725/251‑1 (MQ=255)                                                                                                                                                gCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGAAAAACGTATCATCAACGAAACGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGACGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGACGTCt                                                                                       >  1:208977/1‑251 (MQ=255)                                                                                                                                                                                ggAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGATAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTTGTGGTACTTTCGATATTTCTATTAACGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCGGGTTGAAGAATTCaagaaa                                                        <  1:291429/250‑1 (MQ=255)                                                                                                                                                                                          cGTATCATCAACGAAACGACCGAAGCTGAGCTGGCTTACGGTCTGGACAAAGGAAATGGCAACCGTACTATCGCGGTTTATGACCTGGGAGGTGGAACTTTCGGTAATTCTATTAGCGAAATCGAAGAAGTTGACGGCGCAAAAACCTTCGAAGTTCTGGCAACAAACGGGGATAAACACCTGGGTGGTGCAGACTTCAACCACCGTCTGGTCAACGACCGTGTTGAAGAATTAAAGAAAGATCAGGGCa                                              >  2:331251/1‑250 (MQ=255)                                                                                                                                                                                                               gCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCt                        <  1:344375/251‑1 (MQ=255)                                                                                                                                                                                                                 aGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTgg                      >  1:328475/1‑251 (MQ=255)                                                                                                                                                                                                                   ctgcaatGGCGTACGGTCTGGACACAGGTACTGGCAAACGTACTATCGAGGTTTATGATCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGCCGACGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGGGAACCCCACCTGGGTGGGGCAGCCTTCGACAGACGTCTGATCAACTACCTGGTTGAAGAATCAAAGAAAGATCAGGGCATAGACCTGCGAAACgtaccgcaggc                     >  2:328871/7‑240 (MQ=255)                                                                                                                                                                                                                      cgcTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACCACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg                 <  1:206106/251‑1 (MQ=255)                                                                                                                                                                                                                                     tGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaaga   >  1:6803/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggACAAAGGTACTGTCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaa  <  2:6803/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 TTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAA  >  NC_000913/12476‑12954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑