Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,507	T→C	I115I (ATT→ATC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,507	0	T	C	87.0%	49.1 / 0.7	25	I115I (ATT→ATC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (17/3);  total (21/4)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

GCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAG‑TTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTG  >  NC_000913/12263‑12747                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gCACCACGCCTTCTATAATTGCCTATACCCAGGATGGTGAAACTCTGA‑TTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGAAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTTCAGCGTGATGTTTCCATCATGCCGTTCAAACTTATTGCTGCTGAGAACGGAGACGCATGGTTCGCACTTAAAGGCCAGACAATGGCACCGCGGCAAATCTctgct                                                                                                                                                                                                                                            >  2:118430/1‑251 (MQ=255)       cGCCTTCTATCATTGCATATACCCAGGATGGTGTAACTCTGG‑TTGGTCAGCAGGCTAAACGTCAGGCAGTGACGAACCCGAAAAACACCATGTTTGCGATTCACCGCATGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATAATGCCGTTCAAAATTATTGCTGCTGCTAACGGCGACGCATGGGTCGACGTTAACGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAg                                                                                                                                                                                                                                        >  1:375766/1‑249 (MQ=255)                               aGGATGGTGAAAC‑CTGGGTTGGTCAGCCGGCAAAACGTCAGGCATGGACGACCCCGCAAAACCCCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCACCTTGCCGTACAAAATTATGGCTGCTGATAACGGCGACGCAGGGGTCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCt                                                                                                                                                                                                              <  1:168419/251‑1 (MQ=255)                                  aTGGAGAAACTCTGG‑TTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATATCTGCTGAAGAGCTGAAAAAAATGAAGAAAACCGCTGa                                                                                                                                                                                                            >  1:151618/1‑250 (MQ=255)                                     gTGAAACTCTGG‑TTGGTCAGCCGGCTAAACGTAAGGAAGTGACGAACCCGCAACACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAAAAGAAAAGCCTGACGTTTCCATCATGCAGTTAACAATTATTGCTGCTGATAACGGAGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCTGCAGATCTCTGATGAAGTGCGGAAAAAAATGAAGAAAACCGCTGa                                                                                                                                                                                                            >  1:368451/1‑247 (MQ=255)                                             ctgg‑TTGGTCAGCCGGCTAAACGACAGGCAGTGACGAACCCGCCAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGACAATGGCACCGCCGCAGATCTCTTCTGAAGTGCTGAAAAAAATGAATAAACCCGCTGAAGATTGCCTggg                                                                                                                                                                                                >  2:206106/4‑251 (MQ=255)                                                                           aGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATATCTGCTGAAGTGCTGaaaaaac                                                                                                                                                                                                                            >  2:292988/1‑193 (MQ=255)                                                                           aGTGACGAACCCGCAAAACACCCTGTTTGCGATAAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGaaaaaaa                                                                                                                                                                                                                            <  1:292988/194‑1 (MQ=255)                                                                                cGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCg                                                                                                                                                              >  1:294849/1‑251 (MQ=255)                                                                                            acacCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAAc                                                                                                                                                  >  1:146665/1‑251 (MQ=255)                                                                                                           ttAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGaa                                                                                                                                                                                                                                         >  1:167510/1‑149 (MQ=255)                                                                                                                                    ccAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCg                                                                                                          >  1:73331/1‑251 (MQ=255)                                                                                                                                           gaagaaGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGATGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTCCCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCAGTCTCGCTGGTCt                                                                                                   >  2:350608/1‑251 (MQ=255)                                                                                                                                                  tACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGt                                                                                             >  1:149389/1‑250 (MQ=255)                                                                                                                                                       cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACg                                                                                       >  1:129176/1‑251 (MQ=255)                                                                                                                                                        gTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTACAACGt                                                                                      >  1:105925/1‑251 (MQ=255)                                                                                                                                                                  catcatTCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCAAGAAATTGGCACCGCCGCAGATCTCTGATGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAGGATTACCTGGGTGCACCGGTAACTGAAGCTGTTAACGCCGTACCGGCATACTTTTACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTAGCGCTGGGCTTGAAGTAAAACGTAACATCCACg                                                                            >  2:106077/1‑251 (MQ=255)                                                                                                                                                                   cccgatttgtaaaaaaTTATTGCTGCTGATAACGGCGACGCAGGGGTCGAAGTAAAAGGCCAAAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAAc                                                                             <  1:339698/237‑1 (MQ=255)                                                                                                                                                                   atcatGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGAc                                                                                                                       >  1:36041/1‑207 (MQ=255)                                                                                                                                                                          cGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAaccgacc                                                                    >  1:77320/1‑251 (MQ=255)                                                                                                                                                                           gTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAAc                                                                             >  2:339698/1‑241 (MQ=255)                                                                                                                                                                                aaaTTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGAAAAACGTATCATCAACGAACCGACCGCAGCt                                                              >  1:4049/1‑251 (MQ=255)                                                                                                                                                                                                                       atAAAGGCCAGAAATGGGCACCGCCGCAGATCTCGGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGTTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGt                       <  1:87556/250‑1 (MQ=255)                                                                                                                                                                                                                                 gAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCACTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCGGGCTTACGGTCTGGACAAAGGGACCGGCAACCGTACTATCGCgg             >  2:325194/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTg  >  1:34224/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAG‑TTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTG  >  NC_000913/12263‑12747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑