Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 11,814 G→A intergenic (‑28/‑349) yaaI ← / → dnaK DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091311,8140GA86.2% 67.0 / ‑0.0 29intergenic (‑28/‑349)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (12/13);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGT  >  NC_000913/11584‑12060
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
ccGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGtt                                                                                                                                                                                                                                    <  1:378578/251‑1 (MQ=255)
ccGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCAGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGGGGTAGAGGGATGAATACCCACT‑AGtt                                                                                                                                                                                                                                    >  1:325667/1‑251 (MQ=255)
          gTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACT‑AGTTTACTGCTGa                                                                                                                                                                                                                           <  1:30632/250‑1 (MQ=255)
              aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATaa                                                                                                                                                                                                                        >  1:338796/1‑249 (MQ=255)
                                gagTTGGATGTGGTTCACAATGCGACAGACAGGAAGTTTCAGCGACAGATCGGTGGTGTAGTGACGCGGCATTGCAATGATGCCGAGGAGTGTATGGTCGTTTGTCAGCGCCGTGCAGCACAGCAGCAGGCTAATCGCCAGGCAGGCGGAAATGGTAAAAACGGAGTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGAGGAATACCCCCT‑AGTTTACTGCTGAGAAAGAGAAGGTTCAGGTACgga                                                                                                                                                                                                    <  2:265904/251‑3 (MQ=255)
                                         gCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAAtcttttc                                                                                                                                                                                            <  1:235054/250‑1 (MQ=255)
                                             ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATcttttctttt                                                                                                                                                                                        <  1:58680/250‑1 (MQ=255)
                                               cacaATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTCACTGCTGATAAAGAGAAGGTTCAGGTACTTAATCTTTTCTTTttat                                                                                                                                                                                     <  1:103983/251‑1 (MQ=255)
                                                       gACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAAttt                                                                                                                                                                             <  1:64219/251‑1 (MQ=255)
                                                           aaCAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtg                                                                                                                                                                         >  1:246972/1‑251 (MQ=255)
                                                                           gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAAttt                                                                                                                                                                             <  2:91527/231‑1 (MQ=255)
                                                                           gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAAttt                                                                                                                                                                             >  1:91527/1‑231 (MQ=255)
                                                                                   gTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGattcat                                                                                                                                                 >  1:112107/1‑251 (MQ=255)
                                                                                           cGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTtatc                                                                                                                                         >  1:340133/1‑250 (MQ=255)
                                                                                                      aTTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAAtt                                                                                                                              >  1:141012/1‑251 (MQ=255)
                                                                                                              gacgtcGAGGAGGTGATGGTCGTTTGCCTGCGCCGTGCATCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCGTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTTCTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATTAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCa                                                                                                                       <  2:141012/245‑1 (MQ=255)
                                                                                                                 gcCGAGGAGTTTATGGTCGTTTGTCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAAttt                                                                                                                   <  1:17817/251‑1 (MQ=255)
                                                                                                                        agTTTATGGTCGTTTGCCTGCGCCGTGCTGCACAGCATCAGGCTAATCGACAGGCTGGCGGCAATCGGAAAAACGGGTTTCAAAAGGT‑TTCTCTTAGTGGGAAGAGTAAGAGGGATGAATACCCTAT‑AGTTTACTTCTGATAAAGAGAAGGTTCAGGTCCGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGACTGCAGTTCCTTCTTTATCTGAATATAATAGCTGTCAATTTTACGTCt                                                                                                            >  2:317912/1‑251 (MQ=255)
                                                                                                                                                   cagcacagcaTCAGTCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTgc                                                                                 <  1:198150/251‑1 (MQ=255)
                                                                                                                                                   cagcacagcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCGGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTgc                                                                                 <  1:308766/251‑1 (MQ=255)
                                                                                                                                                                   tAATCGCCAGGCTAGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACaaaaa                                                                  >  1:304024/1‑250 (MQ=255)
                                                                                                                                                                                       aTCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAACTTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCttt                                              >  1:236528/1‑249 (MQ=255)
                                                                                                                                                                                         cGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTtgat                                            <  1:295328/250‑1 (MQ=255)
                                                                                                                                                                                           aaaaaaCGGATTTCATAAGTAGTTC‑CTGAGTGGTAAGATGTATGGGGATGAAGACCCTCT‑ATTTTACTTCTGATAAAGAAAAGGTCCAGGGTCTTAATTTTTTCTTTTTATTAAAATTTTTGGTTGAATACCTTGGCTGCGAATCATTCTTTATCTTAATAAAATTTCTGACAATTTTACGTCTTTTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGAc                                         <  2:26899/250‑1 (MQ=255)
                                                                                                                                                                                                        cATAAGGA‑TTCTCTTATTGGGAAGAGGTAGAGGGATGAATACCCACT‑ATGTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTAAAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGAc                             <  1:324140/250‑1 (MQ=255)
                                                                                                                                                                                                        cATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGAcc                            >  2:107641/1‑251 (MQ=255)
                                                                                                                                                                                                                      ttAGTGGGAAGAGGTAGAGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGGTTACGACCCCATTTAGTAGTc               >  1:132076/1‑251 (MQ=255)
                                                                                                                                                                                                                          tGGGACGAGGTAGAGGGATGCATACCCACT‑AGTTTACTGCTGATAAAGAGAAGGTTCATATACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGATGCGATTCATTATTTATCTGATTAAAATTGATGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAGTTTCTGCGCAAAAGCACAAAAAATTTTTGCATCACCCCATTGATGACGTGGGTTCGGACCACATTTagtagtgaaac           >  2:340687/1‑246 (MQ=255)
                                                                                                                                                                                                                                    tAGGGGGATGAA‑ACCCGCTTAGTTTACTGACGATAAAGAGAAGATTCAGGCACGTAATTTTTTCTTTTTATTACAATTTTTTGATGATTGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATGGCTGACAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTTCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCagtgagt  <  2:64926/250‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
CCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGA‑TTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACT‑AGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATGACGTGGTTTACGACCCCATTTAGTAGTCAACCGCAGTGAGT  >  NC_000913/11584‑12060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑