Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,336 A→G A53A (GCT→GCC)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,3360AG96.0% 62.5 / ‑4.4 25A53A (GCT→GCC) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (13/11);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

CACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGG  >  NC_000913/10096‑10568
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
cacagTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACACAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAAttt                                                                                                                                                                                                                                >  1:30790/5‑251 (MQ=255)
 acagTCAGGCTAAAGAAAACGAATTGCAGTACGCGTGCGCCTTTCAGCGGGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGATCGGCAAAAATTTg                                                                                                                                                                                                                               <  1:246889/248‑1 (MQ=255)
                    cGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGaa                                                                                                                                                                                                             >  1:157674/1‑250 (MQ=255)
                      aaTTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGaaaa                                                                                                                                                                                                           >  2:334081/1‑250 (MQ=255)
                                       gcCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGaataa                                                                                                                                                                                         <  1:334081/251‑1 (MQ=255)
                                                 ggtccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGAATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCag                                                                                                                                                                               <  2:124585/248‑1 (MQ=255)
                                                    ccGAAGAACAAAATCAGCGTAAATTCTCCCAGCAGACCCAGGTTGACCCCATGGAACTGTGTATTTGTCGCATCGTACAGACCCAGTTTCGTCAACAGCAGAATCGCAACCAACTTCAGCCAGAAAGAACCGTAAGAGTTGAAGGCGGTTAAACCGAAAGTGTTGCCTTGTTTGTACGCCAGCAGACCGGCAAAAATTTGCGCCATGCCGCCGTAGAAAATTCCCATGGCAAGAATAATACCGTCCAGAGc                                                                                                                                                                            <  2:379732/251‑1 (MQ=255)
                                                           aaaTAAAAAGCGTAAATACGCCCCACAGACCCATGTAGACACCAAGGAACTGGGCATTTGGCGCATCGGTCAGACCCAGTTGCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATaa                                                                                                                                                                     <  2:316330/249‑1 (MQ=255)
                                                                                cccACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGc                                                                                                                                                >  1:224351/1‑251 (MQ=255)
                                                                                                  cacCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTAGGCATCAGCAGAATCGCAACCAACGTCAGCCAGGAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTATTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGAAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCg                                                                                                                              >  1:26184/1‑251 (MQ=255)
                                                                                                         gAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTAAGCCAGAAAGAACCGTAAGAGGTGAATGCGATTAAACCGCAAGTGTTTCCTTTTTTGTACTCCAGCAGAACAGCAAAAATTAACGCGATACCGCCGTAGAAAATGCACATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATAGTGGTCATGCCGacgacac                                                                                                                       >  2:36429/1‑245 (MQ=255)
                                                                                                          aaCTGTGCATTTTGCGCATCGGTCAGACCCAGTTTCGGCATCATCAGAATCGCAACAAACGTAAGCCAGAAAGAAACGTAAGAGATGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCGGACGGGCAAAAATTAGCGCGCTGCCGACGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCTCGTTGAGCAGGTTAAGCAGATTGGTGGTAATGCCGAAGCCCAt                                                                                                                      >  1:263210/1‑251 (MQ=255)
                                                                                                              gtgCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAgg                                                                                                                  <  1:348456/251‑1 (MQ=255)
                                                                                                                           aTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCg                                                                                                     >  1:66457/1‑251 (MQ=255)
                                                                                                                                              ggCATCAGCAGAATCTCAACGAACGTCAGCCAGAAATAATGGTGAGAGGTGAATGCGGTTAAACCTAAGGTGTTGCCTTTTTGGTACTCCAGCAGACCGGCAAAGATTTGCGCGATGCCGCCGTAGAAATTGCCCATGGCAAGAATAATACCGTCTAGAGCGAAATATCCCACGTTGTGCTGGTTAAGCAGATTGTTGGGCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt                                                                                  <  1:50133/251‑1 (MQ=255)
                                                                                                                                                catcagtagaacgtcaccccacGTCAGTCAGAAAGAACCTAAAGAGAGGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGGACTCCAGCGTACCGGCAAAAATTTGCGCGATGCCGACGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATTCCGAGGCCCATCAGGCCAAGCGGTGCCGGATTAGCCAACTTAGGGTTGc                                                                                <  2:258536/230‑1 (MQ=255)
                                                                                                                                                    agcagAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt                                                                            <  1:327465/251‑1 (MQ=255)
                                                                                                                                                       agAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt                                                                         >  1:231929/1‑251 (MQ=255)
                                                                                                                                                         aaTCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGCGCCGGATTAGCCAACTTAGTGTTACCCATAATTc                                                                       >  1:94438/1‑251 (MQ=255)
                                                                                                                                                                           ccAGAAAGCACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCCAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCAGGATTAGCCAAATTAGTGTTGCCCATAATTCCTCCAAAATCCTCATCg                                                      >  2:38405/1‑250 (MQ=255)
                                                                                                                                                                             agaaagaaCCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaat                                                   >  1:315375/1‑251 (MQ=255)
                                                                                                                                                                                aagaaCCGTAAGAGGTGAATTCGGTTAAACCGAAAGTGGTGCCTTTTTTGTACCCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGGTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatgaa                                                <  2:66457/251‑1 (MQ=255)
                                                                                                                                                                                         aaGAGGTGAAGGCGGTTAAACCGAAAGTGTTGCCTTTTTTGAACTCAAGCAGATCGGCAAAAATTTGAGAGATGCCGCCGTAAAAAAAGACCATGGCAAGACTAATAAAGTACGGAGAGCAATAACCAACGATGTGCAGGTTAAGCAGAATGACGGTCATGCCAAAGCCCAACAGGCCAAGCGGCGCCGGCTTAGCTAACTTAGTGTTGCCCATAATTCTTCAAAAATCATCAACGAACGGATGGAGAaat                                       >  2:248016/1‑251 (MQ=255)
                                                                                                                                                                                            aggggAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGCAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTCAACAGATTGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGGGAaatcat                                    >  2:373262/5‑248 (MQ=255)
                                                                                                                                                                                                                               ttGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCTGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAAGCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACAGTAGTGTTTTCAGGGCGCgg  <  2:315375/250‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        
CACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGG  >  NC_000913/10096‑10568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑