Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 10,260 G→A L79L (CTG→TTG)  satP ← acetate/succinate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,2600GA96.4% 70.8 / ‑3.8 28L79L (CTG→TTG) satPacetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (13/14);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

ATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTC  >  NC_000913/10019‑10499
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
aTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCa                                                                                                                                                                                                                                        <  1:112859/251‑1 (MQ=255)
     accattCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGTAACTGTACATGTGGCGCATCTGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCagaaag                                                                                                                                                                                                                                   <  1:86162/246‑1 (MQ=255)
      ccAATCCAGCCGGCAAAGGGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGACAACCAGCACAGTCAGGCTAAAGAACACGAATTGCAGAACGCGTGCGCCTTTCAGCGAGCCGAAGGACATAAACAGCGTAAATACGCCCCACAGAACAAGGTAGACACACAGGAAATGGGCATATAGCACATCGGTCCGAACAAGTTTCGGCATCAGCAGAATCGCCACCCACGTCAGCCacaaca                                                                                                                                                                                                                                   >  2:373606/1‑245 (MQ=255)
                   cAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTg                                                                                                                                                                                                                     >  1:44867/1‑251 (MQ=255)
                         ggATGATTGCGGCTACACCGACAATGATACCGATCGCCTGCAGGGCAATCAGCACAGTCAGGCTAAAGAAAACGAATTGCAGTACGCGTGCGCCTTTCAGCGAGCCGAAGAACATAAACAGCGTAAATCCGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCAATTGGCGCATCTGTCAGAACCAGTTTCGGCATCAGCAGAATCGCATCCAACGTCAGCCAGAAATATCCGTAAGATGTTAATGCg                                                                                                                                                                                                               <  2:160/251‑1 (MQ=255)
                             gtttgcgggtttgccggtcaTGTTTCCGATCGCCATCAGGGCAAACAGCACAGTCAGGATTAAGATAACTAATTGCAGAACGCGTGCGCCGTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACTCCCCACAGACCCAGGTAGACAACAAGGAACTGTGCATTTGGCGCATCGGTAAGACCCATTTTCGGCAGCAGCAGAATAGCAACCAAAGTCAGCCAGAAAGAACCGTAATAGTTGAATGCGGTTa                                                                                                                                                                                                           <  2:313616/232‑1 (MQ=255)
                              atTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACAAAGGAACTGTGCATTTGGCGCATCGGTCAGACACAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACAGTAAGAGGTGAATGCGGTTa                                                                                                                                                                                                           >  1:375638/1‑250 (MQ=255)
                              agttcggcgTTACCGTCAATGGTACCGATCGCCAGCAGGGCAAACAGCACAGTCATGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCGTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACCCCCCACAGACCCATGTAGACACCAAGGAACGGTGCAGTTGGCGCAGCGGTCATACACAGTTTCGGCATCAGCATAATCGCAACCAACGTCAGCCATAAAGATCCGGAAGAGTTGAATGCGGTTaa                                                                                                                                                                                                          <  2:382484/247‑1 (MQ=255)
                                   ggcgTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGGCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                                                                                                                                                                                                      >  1:124585/1‑250 (MQ=255)
                                                        gATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACt                                                                                                                                                                                <  1:264057/251‑1 (MQ=255)
                                                                             cacagTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACACAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAAttt                                                                                                                                                           >  1:30790/5‑251 (MQ=255)
                                                                              acagTCAGGCTAAAGAAAACGAATTGCAGTACGCGTGCGCCTTTCAGCGGGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGATCGGCAAAAATTTg                                                                                                                                                          <  1:246889/248‑1 (MQ=255)
                                                                                                 cGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGaa                                                                                                                                        >  1:157674/1‑250 (MQ=255)
                                                                                                   aaTTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGaaaa                                                                                                                                      >  2:334081/1‑250 (MQ=255)
                                                                                                                    gcCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGaataa                                                                                                                    <  1:334081/251‑1 (MQ=255)
                                                                                                                              ggtccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGAATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCag                                                                                                          <  2:124585/248‑1 (MQ=255)
                                                                                                                                 ccGAAGAACAAAATCAGCGTAAATTCTCCCAGCAGACCCAGGTTGACCCCATGGAACTGTGTATTTGTCGCATCGTACAGACCCAGTTTCGTCAACAGCAGAATCGCAACCAACTTCAGCCAGAAAGAACCGTAAGAGTTGAAGGCGGTTAAACCGAAAGTGTTGCCTTGTTTGTACGCCAGCAGACCGGCAAAAATTTGCGCCATGCCGCCGTAGAAAATTCCCATGGCAAGAATAATACCGTCCAGAGc                                                                                                       <  2:379732/251‑1 (MQ=255)
                                                                                                                                        aaaTAAAAAGCGTAAATACGCCCCACAGACCCATGTAGACACCAAGGAACTGGGCATTTGGCGCATCGGTCAGACCCAGTTGCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATaa                                                                                                <  2:316330/249‑1 (MQ=255)
                                                                                                                                                             cccACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGc                                                                           >  1:224351/1‑251 (MQ=255)
                                                                                                                                                                               cacCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTAGGCATCAGCAGAATCGCAACCAACGTCAGCCAGGAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTATTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGAAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCg                                                         >  1:26184/1‑251 (MQ=255)
                                                                                                                                                                                      gAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTAAGCCAGAAAGAACCGTAAGAGGTGAATGCGATTAAACCGCAAGTGTTTCCTTTTTTGTACTCCAGCAGAACAGCAAAAATTAACGCGATACCGCCGTAGAAAATGCACATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATAGTGGTCATGCCGacgacac                                                  >  2:36429/1‑245 (MQ=255)
                                                                                                                                                                                       aaCTGTGCATTTTGCGCATCGGTCAGACCCAGTTTCGGCATCATCAGAATCGCAACAAACGTAAGCCAGAAAGAAACGTAAGAGATGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCGGACGGGCAAAAATTAGCGCGCTGCCGACGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCTCGTTGAGCAGGTTAAGCAGATTGGTGGTAATGCCGAAGCCCAt                                                 >  1:263210/1‑251 (MQ=255)
                                                                                                                                                                                           gtgCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAgg                                             <  1:348456/251‑1 (MQ=255)
                                                                                                                                                                                                        aTCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCg                                >  1:66457/1‑251 (MQ=255)
                                                                                                                                                                                                                           ggCATCAGCAGAATCTCAACGAACGTCAGCCAGAAATAATGGTGAGAGGTGAATGCGGTTAAACCTAAGGTGTTGCCTTTTTGGTACTCCAGCAGACCGGCAAAGATTTGCGCGATGCCGCCGTAGAAATTGCCCATGGCAAGAATAATACCGTCTAGAGCGAAATATCCCACGTTGTGCTGGTTAAGCAGATTGTTGGGCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGtt             <  1:50133/251‑1 (MQ=255)
                                                                                                                                                                                                                                 agcagAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCAt       <  1:327465/251‑1 (MQ=255)
                                                                                                                                                                                                                                    agAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt    >  1:231929/1‑251 (MQ=255)
                                                                                                                                                                                                                                      aaTCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCGGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGCGCCGGATTAGCCAACTTAGTGTTACCCATAATTc  >  1:94438/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
ATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTC  >  NC_000913/10019‑10499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑