Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,099	G→A	T132T (ACC→ACT)	satP ←	acetate/succinate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,099	0	G	A	100.0%	64.3 / NA	23	T132T (ACC→ACT)	satP	acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

CAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACT  >  NC_000913/9871‑10325                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   taaGACGCGCCGTTAGCGAATAAAAACATCCCCCCGAGCGGGGGGATCTCAACACAATTAGTGGGATTCCCCAATCGGCAGAACGGTGAGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGTTAGATTGCACTGGCACACCAGATCAGACCAATCCAGCCGGCACAGAGGATGATAGCGGCGTTACAGGCAATATTACCGCGCGCCAGCAGGGCAAACAGCAAAGTCAGGCTAAAGaaaaagcaat                                                                                                                                                                                                              >  2:246889/2‑245 (MQ=255)     aCGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGc                                                                                                                                                                                                            >  1:258536/1‑249 (MQ=255)         gACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcg                                                                                                                                                                                                      <  1:55271/251‑1 (MQ=255)                gCGAATAAAAATATCCCCCCGAGTGGTGTGATCTCAAAACAATTAGTGGGACTCACCAATCTGCAGAACGGTGGGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCCCTGGCACCACAGATCAGACCAACCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGAAGAACGCGTgcg                                                                                                                                                                                                <  2:264076/250‑1 (MQ=255)                                      gCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACAt                                                                                                                                                                         <  1:4849/251‑1 (MQ=255)                                                  cAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGt                                                                                                                                                                <  1:122245/248‑1 (MQ=255)                                                                             ggCAGAACGATGCGAACAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATCGATTGAACTGGCCCCAAAGATCAGACCAATCCAGCCGGCAAAGTGTATGATTGCAGAGTCACCGGCAATGTTACCGATCGCCAGCAGGGCCAACAGCACAGTCAGGCTAAAGAAACAGAATTGAAGAACGAGTCCGCCTATCAACGAGCCGACACACATAAACAGCGTAAGAAAGCCACAAAGACCCAGGAAGacccc                                                                                                                                  >  2:47849/1‑248 (MQ=255)                                                                                 gAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAgg                                                                                                                              <  1:259495/251‑1 (MQ=255)                                                                                 gAACGGTCAGACCAAACTTCACGTGCAGTTCTTCACCCACCGCCAGATAAATGTCACTGGACCCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGAGCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTACAGCGTGACGAAGAACATAAACAGCGTAAATACGCCCCACAGTCCCAGGTAGACACCAAgg                                                                                                                              <  2:156800/251‑1 (MQ=255)                                                                                                       gTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGAGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAAGTAGACAGCAAGGAACTGTGCATTTAGCGCATCgg                                                                                                        >  2:264057/1‑251 (MQ=255)                                                                                                                     ccATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc                                                                                          >  1:316330/1‑251 (MQ=255)                                                                                                                          gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt                                                                                     >  1:32848/1‑251 (MQ=255)                                                                                                                                  gatTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGa                                                                              >  1:379732/1‑250 (MQ=255)                                                                                                                                                     aTCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCa                                                          <  1:112859/251‑1 (MQ=255)                                                                                                                                                          accattCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGTAACTGTACATGTGGCGCATCTGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCagaaag                                                     <  1:86162/246‑1 (MQ=255)                                                                                                                                                           ccAATCCAGCCGGCAAAGGGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGACAACCAGCACAGTCAGGCTAAAGAACACGAATTGCAGAACGCGTGCGCCTTTCAGCGAGCCGAAGGACATAAACAGCGTAAATACGCCCCACAGAACAAGGTAGACACACAGGAAATGGGCATATAGCACATCGGTCCGAACAAGTTTCGGCATCAGCAGAATCGCCACCCACGTCAGCCacaaca                                                     >  2:373606/1‑245 (MQ=255)                                                                                                                                                                        cAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTg                                       >  1:44867/1‑251 (MQ=255)                                                                                                                                                                              ggATGATTGCGGCTACACCGACAATGATACCGATCGCCTGCAGGGCAATCAGCACAGTCAGGCTAAAGAAAACGAATTGCAGTACGCGTGCGCCTTTCAGCGAGCCGAAGAACATAAACAGCGTAAATCCGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCAATTGGCGCATCTGTCAGAACCAGTTTCGGCATCAGCAGAATCGCATCCAACGTCAGCCAGAAATATCCGTAAGATGTTAATGCg                                 <  2:160/251‑1 (MQ=255)                                                                                                                                                                                  gtttgcgggtttgccggtcaTGTTTCCGATCGCCATCAGGGCAAACAGCACAGTCAGGATTAAGATAACTAATTGCAGAACGCGTGCGCCGTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACTCCCCACAGACCCAGGTAGACAACAAGGAACTGTGCATTTGGCGCATCGGTAAGACCCATTTTCGGCAGCAGCAGAATAGCAACCAAAGTCAGCCAGAAAGAACCGTAATAGTTGAATGCGGTTa                             <  2:313616/232‑1 (MQ=255)                                                                                                                                                                                   atTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACAAAGGAACTGTGCATTTGGCGCATCGGTCAGACACAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACAGTAAGAGGTGAATGCGGTTa                             >  1:375638/1‑250 (MQ=255)                                                                                                                                                                                   agttcggcgTTACCGTCAATGGTACCGATCGCCAGCAGGGCAAACAGCACAGTCATGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCGTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACCCCCCACAGACCCATGTAGACACCAAGGAACGGTGCAGTTGGCGCAGCGGTCATACACAGTTTCGGCATCAGCATAATCGCAACCAACGTCAGCCATAAAGATCCGGAAGAGTTGAATGCGGTTaa                            <  2:382484/247‑1 (MQ=255)                                                                                                                                                                                        ggcgTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGGCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCg                        >  1:124585/1‑250 (MQ=255)                                                                                                                                                                                                             gATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAACGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACt  <  1:264057/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   CAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAATCGCAACCAGCGTCAGCCAGAAAGAACCGTAAGAGGTGAATGCGGTTAAACCGAAAGTGTTGCCTTTTTTGTACT  >  NC_000913/9871‑10325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑