Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,006	G→A	S163S (AGC→AGT)	satP ←	acetate/succinate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,006	0	G	A	94.1%	41.4 / ‑4.0	18	S163S (AGC→AGT)	satP	acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/7);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

CGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGA  >  NC_000913/9761‑10249                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACAAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGc                                                                                                                                                                                                                                                >  1:160/1‑251 (MQ=255) cGTTGTGGTACACGGTATATTTGCCAGCGTACCGTACTGCAATCAGTTGCTGGAAGGGCCAGACGTTGAAACGGGTACAGAAGTGGTTGCTGCAGTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGATCGGGGGTATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTGAGTACTTCACCCATCGCCAGATAGATTGCACTGGc                                                                                                                                                                                                                                                <  2:139644/251‑1 (MQ=255)                                                           ccATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACAAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCACAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATg                                                                                                                                                                                      <  1:21769/251‑1 (MQ=255)                                                                            accagAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCcagc                                                                                                                                                                       >  1:250437/5‑249 (MQ=255)                                                                                     ggTTGCTGAATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACa                                                                                                                                                             <  2:7056/250‑1 (MQ=255)                                                                                                               taaGACGCGCCGTTAGCGAATAAAAACATCCCCCCGAGCGGGGGGATCTCAACACAATTAGTGGGATTCCCCAATCGGCAGAACGGTGAGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGTTAGATTGCACTGGCACACCAGATCAGACCAATCCAGCCGGCACAGAGGATGATAGCGGCGTTACAGGCAATATTACCGCGCGCCAGCAGGGCAAACAGCAAAGTCAGGCTAAAGaaaaagcaat                                                                                                                                  >  2:246889/2‑245 (MQ=255)                                                                                                                   aCGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGc                                                                                                                                >  1:258536/1‑249 (MQ=255)                                                                                                                       gACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAAcg                                                                                                                          <  1:55271/251‑1 (MQ=255)                                                                                                                              gCGAATAAAAATATCCCCCCGAGTGGTGTGATCTCAAAACAATTAGTGGGACTCACCAATCTGCAGAACGGTGGGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCCCTGGCACCACAGATCAGACCAACCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGAAGAACGCGTgcg                                                                                                                    <  2:264076/250‑1 (MQ=255)                                                                                                                                                    gCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACAt                                                                                             <  1:4849/251‑1 (MQ=255)                                                                                                                                                                cAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGt                                                                                    <  1:122245/248‑1 (MQ=255)                                                                                                                                                                                           ggCAGAACGATGCGAACAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATCGATTGAACTGGCCCCAAAGATCAGACCAATCCAGCCGGCAAAGTGTATGATTGCAGAGTCACCGGCAATGTTACCGATCGCCAGCAGGGCCAACAGCACAGTCAGGCTAAAGAAACAGAATTGAAGAACGAGTCCGCCTATCAACGAGCCGACACACATAAACAGCGTAAGAAAGCCACAAAGACCCAGGAAGacccc                                                      >  2:47849/1‑248 (MQ=255)                                                                                                                                                                                               gAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAgg                                                  <  1:259495/251‑1 (MQ=255)                                                                                                                                                                                               gAACGGTCAGACCAAACTTCACGTGCAGTTCTTCACCCACCGCCAGATAAATGTCACTGGACCCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGAGCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTACAGCGTGACGAAGAACATAAACAGCGTAAATACGCCCCACAGTCCCAGGTAGACACCAAgg                                                  <  2:156800/251‑1 (MQ=255)                                                                                                                                                                                                                     gTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGAGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAAGTAGACAGCAAGGAACTGTGCATTTAGCGCATCgg                            >  2:264057/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc              >  1:316330/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCAt         >  1:32848/1‑251 (MQ=255)                                                                                                                                                                                                                                                gatTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGa  >  1:379732/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGA  >  NC_000913/9761‑10249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑