Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	9,623	C→A	I106I (ATC→ATA)	mog →	molybdopterin adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,623	0	C	A	93.1%	75.3 / ‑1.7	30	I106I (ATC→ATA)	mog	molybdopterin adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (15/12);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

ATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGT  >  NC_000913/9378‑9844                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              aTCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCt                                                                                                                                                                                                                          >  1:28312/1‑251 (MQ=255)   cccTGCGCTGGAAGAATGGCTGACATCGGCGATAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCCGTAGCGGACCCCGAGATGCCTGGCCTTGGTGATCAGATGGTCCAGATAAACCTGc                                                                                                                                                                                                                        >  2:190171/1‑251 (MQ=255)             gaagaaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATTCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTAcc                                                                                                                                                                                                              <  1:267869/251‑1 (MQ=255)                          cATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAACCGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGATGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGCAGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGCGAACAGATGAGCCAGCTAAGCCTGCATTTTGTACAAACTGCGCTCCtt                                                                                                                                                                                                 >  1:211560/1‑251 (MQ=255)                               gcgcTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCCACTGCGATCCTTTcgcg                                                                                                                                                                                            >  1:184100/1‑251 (MQ=255)                                   tAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTc                                                                                                                                                                                          >  1:156800/1‑249 (MQ=255)                                    aaCCACGCCGTTTGAACTGGAAATCCGCTTAAGCCCCTATGAGCAGTCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCTCGTCGGGACGTAACGCCCGTTGCGTCGCTGGCAGTAGCGGACCGCGTGATGCCTGGCTTTGGTGAACATATGCGCCAGATAAGCCTGCATTGTGTACCAACTGCGATCCGTTCGCGTCa                                                                                                                                                                                         <  2:82837/249‑1 (MQ=255)                                                                        cGATGAGCAGGTGATCATCGAGCAAACGTTGTGGGGGCGAGTGGATGAAATGTGTTGGCGTCTGGGGCACACCACTGGCGGAACGTGTCCGGCACGTTGTGACGTAACGCCCGATGCGTAGCTGGCATTAGCGTACCGCGAGATGCCTGGCTTTGGTGAACACATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTAGCGTTAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACt                                                                                                                                                   <  2:28312/251‑1 (MQ=255)                                                                              gCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCg                                                                                                                                             <  1:356758/251‑1 (MQ=255)                                                                                                                     gggaaTGAGTTGCCATCTGGTTCCCACCTCGGGCGGAACTTGCCCGGCGCGTCCTGAATTACCGCCCGATGCGACGCTGGCAGTAGCGGACCGCGCGATGACTGGCTTTGGTGAACAGAGGCTCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTGTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGa                                                                                                      <  2:106894/248‑1 (MQ=255)                                                                                                                                     cTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTAACCGGTCAGCCGAAGGCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTa                                                                                       >  1:213693/1‑250 (MQ=255)                                                                                                                                                  cGGGCGGAATTGGCTCGGCGCGTCGTGCCGTAACGCCCGATGCGACTCTTGAAGTAGCTGACCGCGAGATGCATGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTGTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTaaac                                                                         <  2:184100/251‑4 (MQ=255)                                                                                                                                                        gAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTAtt                                                                   >  1:215260/1‑251 (MQ=255)                                                                                                                                                                    cgcgTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGcgt                                                        >  1:139644/1‑250 (MQ=255)                                                                                                                                                                     gcgTCGTGACGTAACGCCCGATGCGACTCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGCTCAGCCTGCCTTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTACCTTAACCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTCACGTTGTGGTACACGGTATTTTTGCCAGcgtac                                                      >  2:135283/1‑251 (MQ=255)                                                                                                                                                                                     cccGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGtt                                      <  1:93926/251‑1 (MQ=255)                                                                                                                                                                                      ccGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTg                                     <  1:135283/251‑1 (MQ=255)                                                                                                                                                                                       cGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGc                                    <  1:293124/251‑1 (MQ=255)                                                                                                                                                                                        gaagctaagCTGGCATTCGCGGACCACGTGATGCCTGGCTTTTGTTAACATATTCGCCAGATTAGTCTTCATTTTGTACCAACTGCGATCCTTTCTCGTCATGTTGGTTTGATTCGCAAACAGGCGCTGATCCTGAACTTACCCCGTCAGCCGAAGTCTATTAAAGATACTCTGGAAGTTTGGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCTTACCTTACTGTATTCAGTTGcg                                   <  2:215260/243‑2 (MQ=255)                                                                                                                                                                                          tGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTgg                                 >  1:264076/1‑251 (MQ=255)                                                                                                                                                                                               cGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAggg                            <  1:302597/251‑1 (MQ=255)                                                                                                                                                                                               cGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAggg                            >  1:150948/1‑251 (MQ=255)                                                                                                                                                                                                      tgTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCAAGATAAGCCTGCATTTTGTACCCACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTACAGAGCCGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGGTGTGGTACACGGTa                                                                     >  2:36627/2‑203 (MQ=255)                                                                                                                                                                                                      aGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTa                                                                     <  1:36627/203‑1 (MQ=255)                                                                                                                                                                                                      aGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCACTGATCCTTAACTTACCCGGTCAGCCGGAGTCTATTAAAGAAACGCTGGAAGGGGTGAAGGACGCTGCGGGTAAAGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTGCTGCATTCAGTTGCTGGAAGGGCCATACg                     >  1:343898/1‑251 (MQ=255)                                                                                                                                                                                                       gTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGt                    >  1:382484/1‑251 (MQ=255)                                                                                                                                                                                                                  cgAGATGCCTGGCTTTGGTGACCAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGACGGGCCATACGTTGAAACGGCAc         >  2:112859/1‑251 (MQ=255)                                                                                                                                                                                                                         ccTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGt  <  1:315036/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAGATGCGCCAGATAAGCCTGCATTGTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTGGCTGGAAGGGCCATa                       <  1:101280/215‑1 (MQ=255)                                                                                                                                                                                                                                        cAGATCCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGAGCTGATCCTTAACTTACCCGGTCAGCCGCAGTCTATTAAAGAGACGCTGGAAGTTGTTAAGGACGCTGCGGGTAACGTTGTGGGACACGGCATTTTTGCCAGCGTACAGTACTGCATTCAGTTGCTGGAAGGGCCATa                       >  2:101280/1‑215 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                              ATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGT  >  NC_000913/9378‑9844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑