Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	9,488	G→A	L61L (CTG→CTA)	mog →	molybdopterin adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,488	0	G	A	100.0%	67.9 / NA	25	L61L (CTG→CTA)	mog	molybdopterin adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

GTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCG  >  NC_000913/9246‑9705                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          gtcACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAAGGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGAt                                                                                                                                                                                                                     <  1:369079/249‑1 (MQ=255)         cgttgCAGTGTATCATTCTGGTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTTTTGGCTTAGTTTCCATCTCTGATTGCGCTTCCAGCGTCGTTTACCAGTATAAATGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGTCGTTTGAACTGGAACCCTGCTTATTCCCCGATGAGCAGGAGATCATCGAGCAAACGTTGTGTGATCTAGTGGATGAAATGAg                                                                                                                                                                                                             <  2:28805/245‑1 (MQ=255)         cTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGtt                                                                                                                                                                                                           >  1:191783/1‑251 (MQ=255)             gCAGTGTATCATTCTGTATAACGAGATTGTTTTAACGGTAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCCCTGTCCGCGCACCCAGCGGCGTTTATCAGGATAACGGCTTCCCTGCGCTGGAAGCATGGCTGAGCGCGGCGCTAACCACGCCGCTTGACCTGGAAACCCGTTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCa                                                                                                                                                                                                       <  1:229462/251‑1 (MQ=255)               aGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGCTCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACACCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCACACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATc                                                                                                                                                                                                     >  1:205190/1‑251 (MQ=255)                gtgtATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCt                                                                                                                                                                                                    >  1:27194/1‑251 (MQ=255)                     tCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGc                                                                                                                                                                                               >  1:131421/1‑251 (MQ=255)                                            tAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGccc                                                                                                                                                                         <  2:27194/250‑1 (MQ=255)                                                               gaATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACg                                                                                                                                                      <  1:40973/250‑1 (MQ=255)                                                                            aTTGGCTTAGTTTCCAACTCTGATCGCGCATCCCGCGGCGTTTAACAGAATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCGAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGTGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCTTGATGCGACGCt                                                                                                                                        <  2:297588/251‑1 (MQ=255)                                                                                     gTTTCGATCTCTGAGCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTGTGAACTGGAAACCCTCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGc                                                                                                                               <  2:131421/251‑1 (MQ=255)                                                                                     gTTTCCATCCCTGATCGCGCAGCAAGCGGCTTTTATCAGGATAAAGGAATCCCTGCGCTGGAAGATGGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAACCGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGAGGCGACGCTGGCAGTAGc                                                                                                                               <  1:152145/251‑1 (MQ=255)                                                                                                     gcgcATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGCCCGCGAGATGCCt                                                                                                               >  1:280457/1‑251 (MQ=255)                                                                                                     gcgcATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCt                                                                                                               <  1:171149/251‑1 (MQ=255)                                                                                                             agctgcgTTGATCAGGACAAATGCAACCCTGCCCTGGAAGAAGGTCTGGCAGCGGCGCTAACCACGCAGGTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCAGCGAGCAAACGGTGTGTGAGCGAGTGGATTAAATGAGTGGCCATCTGGTGCTCACCACGGGCGTAACTGGCCCTGCTCGTCGTGACGTAACGCTCGATGCGACTCTGGCAGTTGCGGTTCGCTAGATTCTTGGCTTTgg                                                                                                       <  2:215528/247‑1 (MQ=255)                                                                                                                 gcgTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGCGATGCCTGGCTTTgggga                                                                                                    >  1:214258/1‑247 (MQ=255)                                                                                                                                     aTCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCt                                                                               >  1:28312/1‑251 (MQ=255)                                                                                                                                       cccTGCGCTGGAAGAATGGCTGACATCGGCGATAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCCGTAGCGGACCCCGAGATGCCTGGCCTTGGTGATCAGATGGTCCAGATAAACCTGc                                                                             >  2:190171/1‑251 (MQ=255)                                                                                                                                                 gaagaaTGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATTCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTAcc                                                                   <  1:267869/251‑1 (MQ=255)                                                                                                                                                              cATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAACCGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGATGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGCAGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGCGAACAGATGAGCCAGCTAAGCCTGCATTTTGTACAAACTGCGCTCCtt                                                      >  1:211560/1‑251 (MQ=255)                                                                                                                                                                   gcgcTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCCACTGCGATCCTTTcgcg                                                 >  1:184100/1‑251 (MQ=255)                                                                                                                                                                       tAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTc                                               >  1:156800/1‑249 (MQ=255)                                                                                                                                                                        aaCCACGCCGTTTGAACTGGAAATCCGCTTAAGCCCCTATGAGCAGTCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCTCGTCGGGACGTAACGCCCGTTGCGTCGCTGGCAGTAGCGGACCGCGTGATGCCTGGCTTTGGTGAACATATGCGCCAGATAAGCCTGCATTGTGTACCAACTGCGATCCGTTCGCGTCa                                              <  2:82837/249‑1 (MQ=255)                                                                                                                                                                                                            cGATGAGCAGGTGATCATCGAGCAAACGTTGTGGGGGCGAGTGGATGAAATGTGTTGGCGTCTGGGGCACACCACTGGCGGAACGTGTCCGGCACGTTGTGACGTAACGCCCGATGCGTAGCTGGCATTAGCGTACCGCGAGATGCCTGGCTTTGGTGAACACATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTAGCGTTAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACt        <  2:28312/251‑1 (MQ=255)                                                                                                                                                                                                                  gCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCg  <  1:356758/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                          GTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTATCAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTTGAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCG  >  NC_000913/9246‑9705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑