Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,996	G→T	A253A (GCG→GCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,996	0	G	T	95.5%	58.4 / ‑5.1	23	A253A (GCG→GCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (9/12);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8756‑9216                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                             ccTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGCGCTGGCTGAGAGCGa                                                                                                                                                                                                                      >  1:323583/1‑249 (MQ=255)            ccatggttgGCCGTATTCTTTACTGGTACAAAGCGAATACCGATAAGAA‑AGAATAAGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATATGAAATCTACCAGTACTACAAAAAGCCCGGTTATGAACCCGTGGTTATGGGCGAAAGCTTCCTTAACATCGGCGAAATTCTGGAAATGGCATACTGAGTACTG‑CTGACCATAGCACATACACTGCGGAAAGAGATGGCAGAGGGCGAAGGGACTATCGa                                                                                                                                                                                                          >  2:205765/6‑250 (MQ=255)             ggttgttgGCCGTATGCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGagag                                                                                                                                                                                                                         <  2:273625/232‑1 (MQ=255)             gTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGagag                                                                                                                                                                                                                         >  1:273625/1‑234 (MQ=255)                          aTTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTa                                                                                                                                                                                           <  1:259226/251‑1 (MQ=255)                          aTTCGTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTa                                                                                                                                                                                           <  1:236379/251‑1 (MQ=255)                              gagggttggaaaaaGCGATTAGCGATA‑GAACAGAGTGCGCGCGGGCAGAAGAGGCGGGCGTGGTTTCTGTAGCTGAAACGTACTAGTACACCAAAGAGCGGGGGTATGAAACGGGGGTTTTGGGGGCAAGCTGCGGTAAAAGCGGCGAAATTCGGGAACTGGCAGGCTGCG‑GCCGTCTGTGCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                                                       <  2:259904/240‑1 (MQ=255)                               tGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                                                       <  1:267408/250‑1 (MQ=255)                                                      aTAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgcg                                                                                                                                                               >  1:328906/1‑251 (MQ=255)                                                                agTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACt                                                                                                                                                      <  1:65202/251‑1 (MQ=255)                                                                              gaagaTCCGGGCGTGGTTTCTGTATCTGAAATCTAACAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCt                                                                                                                                        >  1:215528/1‑251 (MQ=255)                                                                                 gaTCCGGGCGAGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAAAATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgtg                                                                                                                                     >  1:98382/1‑251 (MQ=255)                                                                                                tctttATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGACATGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                       <  2:66311/246‑1 (MQ=255)                                                                                                               tcccAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGGGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTATTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGCTCCAATGGCAGTAg                                                                                                        <  1:139893/248‑1 (MQ=255)                                                                                                                       ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTgg                                                                                               >  1:59470/1‑251 (MQ=255)                                                                                                                                                  ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                    <  1:107483/251‑1 (MQ=255)                                                                                                                                                         ggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAgg                                                              >  1:102706/1‑250 (MQ=255)                                                                                                                                                                  ttCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGCCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTgg                                                     >  1:164022/1‑250 (MQ=255)                                                                                                                                                                      ggaatatatgagAAATTTTGGAACTGGCGGGTTGCG‑ACAGTATTGCCAGCGCACCTACACGGCTGAAAGAGGTGGCTGAGAGCGAAGGGGCTATCTAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCTCGTCCGGCTGGTATCGCTGAGTCCGAGTTCCGGTGGCAGCCCAACCATGATCCAATGGCAGTAGAAAATGTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACGGGaaaaa                                                <  2:149523/240‑1 (MQ=255)                                                                                                                                                                                                  aGGCTGCG‑ACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGCGTTCCTGTGGAAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTGTTGACCAAGAAAAACGGGAAAAAAAGAACGGCGATTCGCTGTAATCATTCt                    >  2:96581/1‑251 (MQ=255)                                                                                                                                                                                                   ggCTGCG‑ACAGTCTTACCATCACACCTACACTGCTGAAAGAGCTGGCTGAGGGCGAAGGGGCTATCGAACGTAAACTGTCTTACTCCGGCGAAGTTAAAGCGCGTCCGGCGCTTATCAATGGGTCCGAGTGTCTGTTGCAGCTCAATCAGTATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCtt                   <  2:121953/251‑1 (MQ=255)                                                                                                                                                                                                            ccGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGGCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGgc                                                                                                                                   <  1:218594/131‑1 (MQ=255)                                                                                                                                                                                                                     cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTTTCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTc  <  1:269769/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                             CCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAA‑AGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCG‑ACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8756‑9216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑