Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,711	A→T	A83A (GCT→GCA)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,711	0	A	T	86.7%	34.4 / NA	17	A83A (GCT→GCA)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/2);  major base T (3/10);  minor base G (0/2);  total (3/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAG  >  NC_000913/7496‑7945                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            cctagggCGATGAGCAAAAAGACGGAGAACAGAACCCCCACCCAGCCCACCCCCACCCCGCGCACCATACACCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCAAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATGGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                                                                          <  2:47460/246‑1 (MQ=255) ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                                                                                                                          <  1:226794/248‑1 (MQ=255)     aGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAcgcg                                                                                                                                                                                                       <  1:228219/250‑1 (MQ=255)         gATGAGCAAAAAGTCGGTGAACAGAGCCCTCACCCCGCGCATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCTCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGTTTTGCGAGGGAAAATTCGGCAAACGAGATCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGcgccg                                                                                                                                                                                                  <  1:227788/251‑1 (MQ=255)                                      ccATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCCCCACCCACCCGCGCCGCACAG‑CCGGGCCACAATGACTGAAAtagg                                                                                                                                                                    >  1:334545/1‑248 (MQ=255)                                            aGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTTACCGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGACTGGGACACAATTACTGAAACGAGGttca                                                                                                                                                               >  1:358515/1‑248 (MQ=255)                                             gcgcATCCCCAGCCCGCGCGCCATAGACGATGCCGGGCCGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAAc                                                                                                                                                             <  1:260138/251‑1 (MQ=255)                                                  cccccAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCt                                                                                                                                                        <  1:132373/250‑1 (MQ=255)                                                                                      cACGAAACTGCCCATTGGCATAACGTTCTTTATAAAGCGGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCa                                                                                                                    <  1:263307/251‑1 (MQ=255)                                                                                                                   ttATAAAGCTGTGCGAGGAAACATTCGGCAACCGAGTGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGcaca                                                                                         <  1:181972/249‑1 (MQ=255)                                                                                                                                cgagggaACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTTCCAGCGCAACACCGGCCAGGGTGCCGCTCACCACGCGCGCCGCA‑AGACTTGTACAAAATGACTGAAATGAGGTTAAAACGCCCGGCTGTGGGTGAATGCTATTTTTAAAACTTTTGCCAAACTGGCGGATGTAGCGGAAAGACACAAATCCGGGGCGaaa                                                                           >  1:123090/6‑250 (MQ=255)                                                                                                                                         agtCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGaa                                                                            <  1:16061/238‑1 (MQ=255)                                                                                                                                                  ataccttacgccgggcGATAAACGCGGCATCCCGCTTCCGGAAGACGGCCCCAGGGCCGCAGGCGGTAATGGCCAGGTCTAGCCCGGCCAGGTTGCCGCTACCCACGCCGGCCGAA‑AGACGGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTgcg                                                        <  2:224478/236‑1 (MQ=255)                                                                                                                                                    gAGGTCGCCCTGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGcc                                                      <  1:353646/251‑1 (MQ=255)                                                                                                                                                        tCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCGCCGTCGGTAATATCCGGCGCAACGCCGGCCAGGTTGCGGCTACCCACGCGTGCCGCA‑AGACTGGAGCACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCCATTTTTAAGGCGTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGaa                                                   <  1:364134/250‑1 (MQ=255)                                                                                                                                                         cGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAaga                                                 <  1:197303/251‑1 (MQ=255)                                                                                                                                                                                                        gTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGgag  <  1:61297/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAG  >  NC_000913/7496‑7945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑