Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,0670C.66.7% 9.3 / 17.1 15intergenic (+47/‑167)thrC/yaaXthreonine synthase/DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base . (4/6);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAG‑CGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATG  >  NC_000913/4832‑5290
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                 
ccgtgcgtatatGGCTTGTTCCTCGGCACCGCGCATCTGGTGAATTTTAAAGAGAG‑CGTGGAAGTGTTTCTCGGTGAATCGTGGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATgaaga                                                                                                                                                                                                                   <  1:361221/243‑1 (MQ=255)
                 gTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAG‑CGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTTCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGTCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGaaaaac                                                                                                                                                                                                   <  1:384844/250‑2 (MQ=255)
                       cgtacacggtcaaccgtcaaaaTTTAAAG‑GAGGCTTGGAGGCGACTCGCGGTGAAACGTTGTTTCTGCAAAAAGAGCGTGAATAATGTGCTGTTTTCCTCTGGTTTTAAAATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GTCTTTTTTATGAAGAAATTATGGAGAAAAACGACAg‑gg                                                                                                                                                                                           <  2:331417/232‑1 (MQ=255)
                          cACCGCGCATCCGGCGAAATTTAAAGAGAG‑CGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGaa                                                                                                                                                                                         >  1:25864/1‑250 (MQ=255)
                                  atccggaaaaataaaaaGAGGG‑CGGGAAAGCGTTTCTCGGTGAAACGTTGGGTCTGCAAAAAGAGCGGACAGAACGTGCTTATTTACCCTTACTTTCACATATTCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGTCGGGTTTGCTTTTATGCAGCCCGGCTTTGTTATGAAGAAATTATGGAGAAAAATGACAG‑GGAAAAAGgaga                                                                                                                                                                                 <  2:166399/237‑1 (MQ=255)
                                    ccGGCGAAATTTAAAGAGAG‑CGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGCAATTACGGAGAAAAACGACAG‑GGAAAAAGGAGAAAt                                                                                                                                                                              >  2:88561/1‑251 (MQ=255)
                                          aaaTTTAAAGAGAG‑CGTGGAAGCGATGCTCGGTGAAAAGGTGGATCTGCCAAAAGGGCTGGCAGAACGTGCTGATTTACCCTTGTTTTAACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGAAAAAGGAGAAATTCT‑Caa                                                                                                                                                                       <  1:193592/251‑1 (MQ=255)
                                                    gatagcatGGAAGCGATTCTCGGTGAAACATTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGACTCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGAATAAGGAGAAATTTT‑CAATAAATgtgg                                                                                                                                                              >  2:47793/8‑248 (MQ=255)
                                                     agcacGTGGAAGCGATTCTCGGTGACACGTCGGATCTGCCCAAAGAGCTGGCAGAACCTGCTGCTTTACCCTTGCTTTCACATCATCTGCCCGCCGCTTTTGCTGCGTTGCGTAAATTTATGCAGAATCATCATTACAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCA‑TCCGTCTTTTTTCTGAAGAAATTATGTCGAAAAACGCCAG‑GGACAAAGGAGAAATTCT‑CAATAATTGCgtt                                                                                                                                                             >  2:237885/5‑249 (MQ=255)
                                                                                                                      ttACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc                                                                                             >  1:211714/1‑251 (MQ=255)
                                                                                                                                                      ttttGCTGCGTTGCGTAAATTGATGTTGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATTGACCCCTAACGGGCAATGATAAAAGGAGTAAcc                                                            >  1:269373/1‑251 (MQ=255)
                                                                                                                                                                                        caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GTCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGAAAAAGTAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTCTGAAAAAGATGCAATCTATCGTACTCGCACtt                          <  1:213204/251‑1 (MQ=255)
                                                                                                                                                                                                 cTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATCCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                  >  1:22063/1‑251 (MQ=255)
                                                                                                                                                                                                        gtATCCCAATTAGGCCGGGTTTGCCGTTATGTA‑TCCAGTTTTTATATGAAGAAATTATGGAGAAAAACGA‑AGTGTAAAAAGGA‑AAATTTTGCAATAATTGCGGTAACGTCGAGATTAAGACTGAGGATAATAACAACCGGCGTTGTCATTTCGGAATCTCCTGATAGCGTGGCGTATCGTGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATATAGCGTACTCTCACTTTCCCTGGTTCtt              <  2:220562/246‑2 (MQ=255)
                                                                                                                                                                                                                aTCAGGCCGGGTTTGCTTTTGTGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATg  <  1:10166/251‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                 
CCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAG‑CGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAG‑GGAAAAAGGAGAAATTCT‑CAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATG  >  NC_000913/4832‑5290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑