Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,9300GC75.9% 44.9 / 16.6 29E92Q (GAG→CAG) tsrmethyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base C (14/8);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGG  >  NC_000913/4591692‑4592170
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
gctgGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGt                                                                                                                                                                                                                                        <  1:59472/250‑1 (MQ=255)
                  tGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCgg                                                                                                                                                                                                                     >  1:224919/1‑251 (MQ=255)
                         tagacacgtaaatcatgttgTCTGTTAGTTAATGCCGTAAAGCAGGACGAAGAAAATGTCTATTTTCT‑ACAGACCATTCGCCAGCAGCTCTCCACTCTTAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACGCCCTCAATCGCGCGGGTAGCCGCTTAATGAGGGATCAGAATAATAGTTGTAGCGGTTCAACCGTTGGTGAGCTGCTGCATAGTGCCAGTATTTCGCAGAAACTGGCGGaaacaa                                                                                                                                                                                                               <  2:353503/231‑4 (MQ=255)
                           aCAACTGACAGCAGGCGGTCTGTTCTTTAATGGCTTCAAGAATGACAACGAAAATTTC‑CAGTTTTTGCTAACCATTCGCCAGCAGCAAATCTCGCTTAACGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAATACCATCAAACGCGTGGTAACCCGCTGAATGATGGTTCAGAATAATATTTATAGCGTTGCAACCGTTGATGATCTGATGCAGAGTTCTAGTATTTCGCCGAAACAGGCGGaaaaacac                                                                                                                                                                                                             <  2:73502/250‑4 (MQ=255)
                            cAACTGACATCAGGCGGTCTGTTCTTGAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGGTCAGAATACTATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGAGGAAAAAAACTg                                                                                                                                                                                                           >  2:65764/1‑251 (MQ=255)
                                   cATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAACTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACTGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGAt                                                                                                                                                                                                    >  1:40597/1‑251 (MQ=255)
                                                gTTCTTTAATGCCTTAAAGAATGACCAAGAACATTTCACTGTTTT‑ACAAACCATTCGACAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCACGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGAAGCGGTTCAACCGTTGCTGCGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGttac                                                                                                                                                                                       >  1:293453/1‑249 (MQ=255)
                                                     ttAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATACACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAAACGCGCGGGCATCCGCGACATGATGGGTCAGAATAATATTGGTAGCGGTTCAGCCGTTGCTGAGCAGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAAATGGGGCGATTACGCAGTGTTGCAGCGt                                                                                                                                                                                  >  1:330689/1‑251 (MQ=255)
                                                                                            t‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGAGGGATCAGAATAATATTGGGAGCGGTTCAACCGTGGGTGAGCTGATGCAGAGGGCCAGTATTTCGCTGAAACGGGCGGAAAAAAACTGGGCAGATTGCGAAGGGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCaa                                                                                                                                            <  1:172284/250‑1 (MQ=255)
                                                                                                            agcagcAATCCACGCTGAATGGCGGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCCCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGGAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTGCGAAGCGGTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATAtt                                                                                                                            <  1:53118/251‑1 (MQ=255)
                                                                                                                 caATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGTTGGATCAGAATAATCTTGGTAGCGGGTCAACCGTTGCTGGGCTGCTGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAATAAACAGGGCAACTTACGAAGCGTGGCCGCGTGAACCGCGTCAGAGCACCGCCGCGGAGGCAGCGATCAACCGTaaataagaattttttc                                                                                                                        >  2:49360/1‑236 (MQ=255)
                                                                                                                       ccGCTGAATGTCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATgc                                                                                                                 <  1:157386/250‑1 (MQ=255)
                                                                                                                         gttaaagggCAGATGGGTCGCGTTGTGGCAGAAGCGTAACCCCCTCATCAGCGGGGGTATCCGCTAAATGATGGATCAGAATAATATTGGTAGCGGTTAAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAACCTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATgcgc                                                                                                               <  2:40597/244‑1 (MQ=255)
                                                                                                                           tGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTg                                                                                                             >  1:266042/1‑251 (MQ=255)
                                                                                                                                              tttgttgCAGACGCGTACCACCCTCAACCGCGCGGGCCTCCGCTACATGCTGGATCAGCATAATATTGGCAGCGGTTCAACCGTTGCTGCGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCCGATTCCGCAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCCGAGCTCCACCGTAATTACGCTATTTATCACCATGCGCtaccggagactaacccactat                                                                                          >  2:174174/2‑231 (MQ=255)
                                                                                                                                                ggttgcagctgagTAACCCCATCACCCGCGGGGGTCTCCGCTTTATGATGGATCAGAAAAAAATTGGTAGCGGTGCAACCGTTGCTGAGCGGATTCAGTGTGCCATAATTCCCCTGAAACAGGCGAAAAAAAATGGGGCAGATGACAAAGCGTTGCCGCGGGACCCGCGTCAGAGCCCCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTAGCACAAGGCGCTGGCGGAGCTGATCCAACTATTa                                                                                        <  2:224919/239‑1 (MQ=255)
                                                                                                                                                              aaCACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAAt                                                                          >  1:10717/1‑251 (MQ=255)
                                                                                                                                                                cacCCTCAACCGCGCGGGTATCCGCTAAATGATGGATCAGAATAATATTGGTAGCGGTTAAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCa                                                                        <  1:1992/251‑1 (MQ=255)
                                                                                                                                                                  cccTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGAATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGAGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGAGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAAc                                                                      >  1:233252/1‑251 (MQ=255)
                                                                                                                                                                        aCCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCGGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTc                                                                <  1:48817/251‑1 (MQ=255)
                                                                                                                                                                              cgGGTATCCGCTACATGATGGATCAGAATAATATGGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGAt                                                          <  1:65764/251‑1 (MQ=255)
                                                                                                                                                                                             tgatgGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGACAAAAACTGGGCGAATTACGAAGCGTTGCAGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGCGATCAAACGTAATTACGATATTTCTCACACGGCGCTGGCGGAGATGATCCAACTGTTAGGTGCAGGCAAAATCACCGAGATCATTGATCAGCCAGACCCAgg                                            >  2:153345/1‑250 (MQ=255)
                                                                                                                                                                                                        cataataTTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGCGTGCCAGTCTTTCGCTGAAACAGGCGGTAACCACCTGGGCAGATTCCGATGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCGGCGGCAGAGATCATCCGTCATTTCGATATTTATCACAATGAGCTGGCGGAGCTGATCCAACTTTTAAGTGCAGGCTAATTCTACGCGTTCTTTGTTATGCC‑GACCCATGGATATCGGGACgg                               >  2:37362/2‑251 (MQ=255)
                                                                                                                                                                                                         ataataTTGGTAGCGGTTCACCCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAACCAGGCGGAAAAAAACTGGGCGGATTCCGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTGGATCAGCC‑GACCCAGGGATATCAGGACGGt                              <  1:153345/251‑1 (MQ=255)
                                                                                                                                                                                                                            aaCCGTTGGTGAGCTGAGGGAGAGGGCCGGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGTGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GATCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTgg           <  1:147478/251‑1 (MQ=255)
                                                                                                                                                                                                                            aaCCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAACCGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGACGGTTTCGAGAAGAAGTATGTgg           >  1:76699/1‑251 (MQ=255)
                                                                                                                                                                                                                               cGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCtt        >  1:165706/1‑251 (MQ=255)
                                                                                                                                                                                                                                gTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGATGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCttc       >  1:296395/1‑250 (MQ=255)
                                                                                                                                                                                                                                       agctgaTGCAGAGTACCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGCCGGTTTCGAGAAGCAGTATGTGGCTTACATgg  >  1:282295/1‑249 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 
GCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTT‑ACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCC‑GACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATGTGGCTTACATGG  >  NC_000913/4591692‑4592170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑