Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,532,0630CT78.8% 47.9 / 12.2 33intergenic (‑412/+374)sgcX/yjhPputative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (12/14);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTT  >  NC_000913/4531835‑4532297
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          
tATGGTTGAAAGAATGAGGATTAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGTACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAAGCATCTCAAATCATTCTCCGCTTTATGAGCCCAgg                                                                                                                                                                                                                       <  1:97448/250‑1 (MQ=255)
    gtagAAATAATGATGATGAATAAAACGTGGTAACTTTTATAGAAACCCTTTGTTGCAGCACGATACCTCTGCATTACGTCCTGCACGTGGATACCCCAGCAACTTCATCTGCGGAGCTTAGAATGTAATGGCGCGTGTCAGGTCGATGGTTCCCAGCATAATGGAAGAAAACATCGTATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGAACAAATCATTCTCCGCTTTAAGAGCCCAGGCTga                                                                                                                                                                                                                   <  2:301067/247‑2 (MQ=255)
          ataatGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGcc                                                                                                                                                                                                            <  1:300183/251‑1 (MQ=255)
                     gAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAAc                                                                                                                                                                                                  <  1:380177/250‑1 (MQ=255)
                       aTAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGtt                                                                                                                                                                                               >  1:92508/1‑251 (MQ=255)
                          aaaCGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTACATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTg                                                                                                                                                                                            >  2:33738/1‑251 (MQ=255)
                                       gTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTAtgt                                                                                                                                                                                 <  1:87049/249‑1 (MQ=255)
                                       gTTATAGCACCACTTTGTTGCAGGACGCTCCCCCTTCATTACGTCCTGCACGTGGATACCCCAGCAGCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTAtgtg                                                                                                                                                                                <  2:245223/250‑1 (MQ=255)
                                          ataGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGGCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCtt                                                                                                                                                                             <  1:249934/250‑1 (MQ=255)
                                                   cTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGcc                                                                                                                                                                   >  1:106708/1‑251 (MQ=255)
                                                               aCGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGcc                                                                                                                                                        >  1:338400/1‑250 (MQ=255)
                                                                         ttCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGCCTTGGCCTTCAAATAACTTTTTGACCCCTTATGGGCTTCCTCCGGCCCGACTTTTTCCCAGGAATCAAgg                                                                                                                                             >  1:73585/1‑251 (MQ=255)
                                                                                                    cAGCATCTCCATCAGCGGAGCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCGCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGcttct                                                                                                                  <  1:55836/251‑1 (MQ=255)
                                                                                                                atgtggATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTg                                                                                                      <  1:117470/247‑1 (MQ=255)
                                                                                                                    ggATCTTAGAATGTAATGGCGAGTGGCAGGTCGTTGGTTACCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTTGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTCAACCTGAAACCGCTTCTTCCCAGGGtgtg                                                                                                      >  2:117470/1‑244 (MQ=255)
                                                                                                                          tAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGAtt                                                                                                 <  1:140773/246‑1 (MQ=255)
                                                                                                                              aTGTAATGGCGCGTGGCAGGTCGTTGGCTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGCTAACGTTTTGCACGCTTATGTGCTTCCTCCggg                                                                                                                                                                     >  1:75962/1‑174 (MQ=255)
                                                                                                                              aTGTAATGGCGCGTGGCAGGTCGTTGGCTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATTACGTGTTGCACGCATATGGGTTTCCGCCggg                                                                                                                                                                     <  2:75962/174‑1 (MQ=255)
                                                                                                                                gTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGt                                                                                      >  1:81544/1‑251 (MQ=255)
                                                                                                                                          gTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTGATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTa                                                                            <  2:81544/251‑1 (MQ=255)
                                                                                                                                               aGGTCGTAGGTTCCCAGAATAATGTAAGAAAACATCGGATCTACTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTATGCCAGGACTCAAGGAAGCTGTCTAACCCTGCTACCGCTTCTTCCCAGGGTTTGAGATTGCTGTTGCTGACGCGACATTACGccc                                                                       >  2:11990/1‑249 (MQ=255)
                                                                                                                                                   cGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAat                                                                    <  1:329561/250‑1 (MQ=255)
                                                                                                                                                     ttGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATAtt                                                                 >  1:116266/1‑251 (MQ=255)
                                                                                                                                                           cccAGCATAATGTAAGAAAACAACGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAACCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGTACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCa                                                            <  1:23265/250‑1 (MQ=255)
                                                                                                                                                                           aaaaCATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCCCAATATTGATCACTGGCACTGTGTGCATc                                           >  1:254563/1‑251 (MQ=255)
                                                                                                                                                                                   ggATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCg                                   >  1:232109/1‑251 (MQ=255)
                                                                                                                                                                                         ccTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATc                              <  1:179267/250‑1 (MQ=255)
                                                                                                                                                                                                         gggAGTATAGATCATCAGCTCCAATCATTCTCCGCTTTATGAGCCCAGGCTGGCAATGCCGTCAGATAACGTTTTGCCCGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGATAATCCTGATACCGCTTCTTCCCAGAGTTTGAGGTTGCCGGTGCTGTCGCTGCATTTCGCCCCATATTGATCACTAGCACTGTGTGCATATGGCGCCTACAtgtgaatacagcaagcatt             >  2:102504/1‑233 (MQ=255)
                                                                                                                                                                                                               ataGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGa        <  1:33738/250‑1 (MQ=255)
                                                                                                                                                                                                                taGATAATCAGCTCAAATCACTCTCCGATTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTATGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAAc      >  1:35788/1‑251 (MQ=255)
                                                                                                                                                                                                                 aGATAATCAGCTCAAATCATTCCCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACt     <  1:263853/251‑1 (MQ=255)
                                                                                                                                                                                                                  gATAATAAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGCCAGAACTg    >  1:267639/1‑251 (MQ=255)
                                                                                                                                                                                                                    tAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGtt  >  1:252272/1‑251 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          
TATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTT  >  NC_000913/4531835‑4532297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑